MyBioSource.com's Orphan GPCR Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunoprecipitation, and Western Blot. The Orphan GPCR Antibody was generated using GPR3 as the antigen and it reacts with Human, Mouse, and Rat.
Description
Recently a novel human G-protein coupled receptor gene has been characterized and mapped to chromosome 2p16. This gene codes for a 540 amino acid protein in retinal pigment epithelium (RPE) and cells surrounding retinal arterioles. In contrast, the Northern blot data obtained from mouse sections suggest the expression of transcripts in photoreceptor inner segments and I outer plexiform layer. The transcripts of the GPCR-75 gene (7kb) are also found in abundance in brain sections. So far, no mutations in GPCR-75 protein were identified in patients suffering from Doyne's honeycomb retinal dystrophy (DHRD), an inherited retinal degeneration disease that maps to chromosome 2p16 (1). The GPCR-75 protein is approximately 78 kDa (540 amino acids) protein that is primarily expressed in human retinal pigment epithelium (RPEs). The GPCR-75 sequence analyses suggest the presence of 7 trans-membrane domains, a characteristic feature of GPCR. The protein has putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3 intra cellular loop which might be the site for interaction of G-proteins. The short carboxy terminal is intracellular and has putative post-translational modification lipid modification sites. The Anti-GPCR-75-selective antibodies were generated against conserved sequences near N- and C-termini of the protein that are unique to GPCR-75 protein. The polyclonal antibody strongly labels a 78 kDa protein in RPE cell extracts. Anti-GPCR-75-selective antibody is also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses. MyBioSource will also conjugate antibodies with fluorescent probes upon request at extra charge. MyBioSource will also provides antibodies against proteins that are involved in retinal degenerative diseases such as various Anti-PDE antibodies, Anti-MERTK, Anti-Phospho-MERTK, EGF-containing fibulin like intracellular protein (EFEMP1), Anti-Myocilin (TIGR), Anti-Bestrophin, Anti-ELVOL4 and a Usher syndrome specific Anti-USH2a antibodies etc. MyBioSource employs cyclic peptide methodology for generating antibodies, which results in higher titer and specificity (2). MyBioSource will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigens are also available. Please enquire for their availability before ordering