Anti-RhoGDI (phospho S174) antibody ab74142 from Abcam

Product Specs
ItemAnti-RhoGDI (phospho S174) antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab74142
ApplicationsIHC-P, WB
HostRabbit
Conjugate/TagUnconjugated
Quantity100 µg
ClonalityPolyclonal
IsotypeIgG
FormatThe antibody was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site
Concentration1 mg/ml
ModificationsPhospho-S174
Gene NameARHGDIA
ReactivityHuman
NCBI Gene AliasesGDIA1, HEL-S-47e, NPHS8, RHOGDI, RHOGDI-1
Antibody TypePrimary Antibody
Research AreaImmuno-oncology
TargetARHGDIA phospho S174
NCBI Full Gene NameRho GDP dissociation inhibitor alpha

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(1) Poria cocos Ethanol Extract Restores MK-801-Induced Cytoskeleton Regulation in Neuro2A and IMR-32 Cells and Locomotor Hyperactivity in C57BL/6 Mice by Modulating the Rho Signaling PathwayCurrent Issues in Molecular BiologyApril 28, 2025Ya-Ying Chang, Cheng-Wei Lu, Tzu-Yu Lin, I-Shiang Tzeng, Yi-Chyan Chen, Mao-Liang Chenanti-RhoGDI1 (#2564, Cell Signaling Technology, Inc., Danvers, MA, USA); anti-RhoGDI (phospho S174) (ab74142, Abcam Limited, Cambridge, UK); anti-RhoA (67B9) (#2117, Cell Signaling Technology); anti-p-RhoA (Ser188)

(2) Gastrodin Mitigates Ketamine-Induced Inhibition of F-Actin Remodeling and Cell Migration by Regulating the Rho Signaling PathwayBiomedicinesMarch 6, 2025Ping-Cheng Shih, I-Shiang Tzeng, Yi-Chyan Chen, Mao-Liang ChenRhoGDI1 (#2564, Cell Signaling Technology, Danvers, MA, USA), phosphorylated RhoGDI1 (phospho S174, ab74142, Abcam, Cambridge, UK), RhoA (#2117, Cell Signaling Technology), anti-CDC42 (#2462, Cell Signaling Technology),

(3) NEK2 Phosphorylates RhoGDI1 to Promote Cell Proliferation, Migration and Invasion Through the Activation of RhoA and Rac1 in Colon Cancer CellsCellsDecember 16, 2024Jeewon Lim, Yo-Sep Hwang, Jong-Tae Kim, Hyang-Ran Yoon, Hyo-Min Park, Jahyeong Han, Taeho Kwon, Kyung-Ho Lee, Hee-Jun Cho, Hee-Gu Lee(sc-138), RhoA (sc-418), GFP (sc-9996), and β-Actin (sc-47778) of Santa Cruz Biotechnology, RhoGDI1 (S174) (ab74142) and 14-3-3 tau (ab10439) of Abcam (Cambridge, UK), NEK2 (610594), Cdc42 (610929), and Rac1 (610651)

(4) The phosphomimetic mutation of syndecan-4 binds and inhibits Tiam1 modulating Rac1 activity in PDZ interaction-dependent mannerPloS oneJanuary 1, 2017Keller-Pinter A, Ughy B, Domoki M, Pettko-Szandtner A, Letoha T, Tovari J, Timar J, Szilak L.Santa Cruz Biotechnology Inc.; A2484 and A2609, Sigma-Aldrich; polyclonal anti-phospho-S174-RhoGDI ab74142, Abcam Ltd; anti-RhoGDI1 (alpha): polyclonal rabbit A-20/sc-360 and monoclonal mouse B-10/sc-13120,

(5) Regulation of RhoA signaling by the cAMP-dependent phosphorylation of RhoGDIαThe Journal of biological chemistryNovember 9, 2012Oishi A, Makita N, Sato J, Iiri T.

Anti-RhoGDI (phospho S174) antibody from Abcam