Fig 1: Minoxidil inhibited the expression of LHs, collagens, and α-SMA. The protein expression was analyzed by Western blotting. (A) The LH1, LH2, and LH3 protein expression in the lungs of mice on day 21 following BLM administration; (B) The COL I, COL IV and α-SMA protein expression in the lungs of mice on day 21 following BLM administration. The relative expression levels in lung tissue samples were normalized to the level of β-actin (Actin). Values are given as the means ±SD, n=6. * P<0.05 vs. the NS group, ** P<0.01 vs. the NS group, *** P<0.001 vs. the NS group; # P<0.05 vs. the BLM group, ## P<0.01 vs. the BLM group.
Fig 2: Characterization of selected circRNAs qRT–PCR analysis of four circRNA expression in 11 colon cancer cell lines and 2 colon epithelial cells (CCD112 and CCD841). Data are presented as mean ± SD (n = 3).RT–PCR products with divergent and convergent primers showing circularization of has_circ_0122319. cDNA, complementary DNA; gDNA, genomic DNA.qRT–PCR evaluated the knockdown efficiency of has_circ_0122319 and has_circ_0097391 and the host gene (PLOD2 and AGTPBP1) expression in SW620 and HCT116 cells transfected with two unique shRNAs (#1, #2). **P < 0.01, Student's t‐test, mean ± SD (n = 3). Exact P‐values are specified in Appendix Table S5.qRT–PCR evaluated the host gene (PLOD2, AGTPBP1, and ISPD) mRNA expression, and immunoblotting evaluated the PLOD2 protein expression in indicated cells. β‐Actin antibody was used as a loading control. Data are presented as mean ± SD (n = 3).Correlation analysis of transcriptome between two independent shRNA groups in SW620 and HCT116 cells. In each scatter plot, the log10‐transformed Transcripts Per Million (TPM) of reads of each gene were utilized for calculating the spearman's coefficient. R represents Spearman's correlation coefficients, and P‐values were calculated by Spearman's correlation test.
Fig 3: Immunofluorescence staining for COL1 and the LH proteins. The expression and localization of the LH2 (A; green), LH3 (B, green), and COL1 (C; red) proteins was confirmed by immunofluorescence staining. Representative images are shown. All images are 100× magnification.
Fig 4: The PLOD expression in human subjects. The expression levels of PLOD1, PLOD2, and PLOD3 mRNA in human serum were determined by qRT-PCR. Data are presented as the means ±SD, n=15, ** P<0.01 vs. the Control group, *** P<0.001 vs. the Control group.
Fig 5: Immunohistochemical staining for COL1 and the LH proteins. The expression of the COLI (A), LH1 (B), LH2 (C), and LH3 (D) proteins. Representative images are shown. All images are 200× magnification.
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