Fig 1: CoREST1 and CoREST2, but not CoREST3 decrease during cortical neurons maturation.(A) Rat embryonic cortical neurons were immediately processed (day 0) or mantained in vitro during 2, 4 and 6 days to detect CoREST1, CoREST3, HDAC1, HDAC2 and LSD1. Values correspond to the mean ± SEM. **p<0.01, *p<0.05 (DIV 0 v/s DIV 6). Statistical analysis performed by Kruskal-Wallis test followed by Dunn’s multiple comparison test. (B) Rat embryonic cortical neurons were mantained in vitro as described above. Total RNA was extracted and semiquantitative RT-PCR was performed for rat rcor2 and rcor3 transcripts; rpl19 was used as reference gene. Values correspond to the mean ± SEM of at least 3 independent experiments. *p<0.05 (DIV 0 v/s DIV 6). Statistical analysis performed by Kruskal-Wallis test followed by Dunn’s multiple comparison test.
Fig 2: rcor2 but not rcor3 is down-regulated during brain development.Total RNA of E.14.5 and E.18.5 embryonic rat brain, and the cortex of adult male rats were subjected to semiquantitative RT-PCR to determine rcor2 and rcor3 mRNA expression. rpl19 was used as reference gene. Values correspond to the mean ± SEM of at least 3 independent experiments. ***p< 0.001, **p<0.01, according to two-way ANOVA and Bonferroni’s posthoc test. # P<0.05, according to one-way ANOVA and Bonferroni’s posthoc test.
Fig 3: CoREST1 and CoREST2, but not CoREST3 are down-regulated during NGF-dependent neuronal differentiation of PC12 cells.(A) Left: representative immunoblots of CoREST1, HDAC1 and LSD1 in equivalent protein amounts of cytosolic and nuclei fractions of control (-NGF) and NGF treated (50 mg/ml during 7 days) PC12 cells. Histone H3 was used as loading control for nuclear fraction and GAPDH for cytoplasmic fraction. Right: graph of relative protein levels of CoREST1, HDAC1 and LSD1 respect to histone H3 levels. Values correspond to the mean ± SEM of at least 3 independent experiments. **p<0.01. Statistical analysis performed by Mann-Whitney test. (B) Semiquantitative RT-PCR was performed for rat rcor2 and rcor3 genes, rpl19 gene was used as reference gene. Left: representative PCR reaction. Right: Quantification of rcor2 and rcor3 mRNA expression levels in control (-NGF) and NGF treated PC12 cells. Values correspond to the mean ± SEM. **p< 0.01. Statistical analysis performed by Mann-Whitney test.
Fig 4: rcor1-3 transcripts expression profile across rat tissues.The panel shows the expression levels (measured in FPKM) of rcor1-3 genes (A) and rcor3 isoforms (B) in the different rat tissues analyzed.
Fig 5: Annotation of rat rcor1-3 transcripts.UCSC Genome Browser images show transcript annotation data at rcor1 (A), rcor2 (B) and rcor3 (C) loci. Gene model tracks of RefSeq and Ensembl are shown in blue and red, respectively. Assembled transcripts by Cufflinks are shown in black. Predicted ELM2 and SANT1/SANT2 domains are highlighted in yellow and red, respectively. Together, the alignments of RefSeq transcripts annotation from human and mouse with the PhyloP genomic conservation scores give the evolutionary information to support the existence of the new identified exons.
Supplier Page from Abcam for Anti-CoREST3 / RCOR3 antibody