Fig 1: Canine adipose-derived mesenchymal stem cells (cAD-MSCs) surface markers expression pattern during in vitro cultivation. cAD-MSCs. The expression pattern was analysed by calculation of fold change of median fluorescence intensity (MFI). The results are based on eight biological replicates, young female canines referred to as elective surgery procedures with no obvious signs of disease. Flow cytometry assessment of positive and negative cAD-MSCs markers showed no statistically significant changes in the expression of the following positive markers: CD90, CD44, CD29, CD105 and CD271. A statistically significant change in expression was observed for CD73 between early (P1–P3) and late (P4–P6) passages. The X-axis represents the passage number (from P1 to P6), whereas the Y-axis displays the average fold change of MFI. MFI fold change was calculated by dividing the median of the appropriate antibody with the median of isotype control. The Whisker box plot was created to present the dynamics of changes between passages. Centerlines denote the median, plus the mean, box limits indicate the twenty-fifth and seventy-fifth percentiles; whiskers represent the maximum and minimum of the acquired values. Individual values are shown in colours to reveal changes for each donor included in the study. Donor 4/20 is presented in light pink, 5/20 in red, 6/20 in green, 7/20 in light blue, 8/20 in purple, 9/20 in orange, 11/20 in grey and 13/20 in dark pink.