Fig 1: In vitro and in situ interactions of Usp19, DNAAF2, NADSYN1, p23/PTGES3, Hsp105 and Bcl‐G with dcTPR proteins. (A) Coomassie blue R staining of overexpressed and purified proteins used in GST pull‐down assay. (B) Western blot analysis of GST–AIP and GST–FKBP51 pull‐down of Usp19, DNAAF2, NADSYN1, p23/PTGES3, Hsp105, Bcl‐G and their truncated versions lacking five utmost C‐terminal residues. (C) PLA of Usp19, DNAAF2, NADSYN1, p23/PTGES3, Hsp105 and Bcl‐G interacting with AIP in HEK293 cells. The asterisk indicates position of truncated p23 on the gel.