Mouse G-CSFR/CD114 APC-conjugated Antibody from R&D Systems, a Bio-Techne Brand

Yes, the G-CSFR/CD114 APC-conjugated Antibody has been shown to work in flow cytometry applications, as evidenced by its use in studies such as those cited in the literature, including the article by Soukup et al. where it was utilized to detect surface proteins in hematopoietic cells.

The G-CSFR/CD114 APC-conjugated Antibody has been employed in research demonstrating the role of granulocyte-colony stimulating factor (G-CSF) in brain repair mechanisms following radiation injury. In the study by Dietrich et al., it was used to assess the involvement of G-CSF-responsive cells in neurocognitive recovery and structural repair in the brain.

The G-CSFR/CD114 APC-conjugated Antibody is specific for mouse G-CSF receptor/CD114, showing less than 1% cross-reactivity with recombinant human GM-CSF receptor alpha and recombinant mouse GM-CSF receptor alpha in direct ELISAs. This specificity is crucial for accurate detection in experimental settings.

In the context of immunohistochemistry, the G-CSFR/CD114 APC-conjugated Antibody has been cited at a dilution of 1:250 in the study by Dietrich et al., which provides a guideline for researchers looking to utilize this antibody in similar applications.