Fig 2: Impact of IL-22 intervention on the immune-exhausted microenvironment in E.m infected mice. (A) Immunohistochemical representative images of CD155, TIGIT and CD8 in the liver tissues of mice at 1-month post-infection. Scale bar: 100μm. (B) Immunohistochemical representative images of CD155, TIGIT and CD8 in the liver tissues of mice at 3-month post-infection. Scale bar: 100μm. (C) Statistical results of the positive area of CD155 in the liver of mice at 1- and 3-month post-infection (n =5). (D) Statistical results of the positive area of TIGIT in the liver of mice at 1- and 3-month post-infection (n =5). (E) Statistical results of the positive area of CD8 in the liver of mice at 1- and 3-month post-infection (n =5). **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, no significance.

Fig 3: IL-22 promotes CD155 expression through the IL-22RA1-STAT3 signaling axis. (A) Representative western blotting images of IL-22RA1, STAT3 and p-STAT3 expression in hepatocytes across experimental groups. (B) Statistical results of IL-22RA1, STAT3 and p-STAT3 expression in hepatocytes across experimental groups (n=3). (C) Immunohistochemical representative images of IL-22, IL-22RA1 and p-STAT3 in the liver tissues of mice at 1-month post-infection. Scale bar: 100μm. (D) Immunohistochemical representative images of IL-22, IL-22RA1 and p-STAT3 in the liver tissues of mice at 3-month post-infection. Scale bar: 100μm. (E) Statistical results of the positive area of IL-22, IL-22RA1 and p-STAT3 in the liver of mice at 1-month post-infection (n =5). (F) Statistical results of the positive area of IL-22, IL-22RA1 and p-STAT3 in the liver of mice at 3-month post-infection (n =5). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, no significance.