Fig 2: Solute carrier protein expression in human FXS NPCs and neurons(A) Western blot data for indicated proteins in FXS and isogenic iControl cells in NPCs and neurons at weeks 1 and 4 of maturation.(B–D) RT-PCR data indicate changes in RNA levels in FXS and isogenic iControl cells in NPCs and neurons at weeks 1 and 4 of maturation (N = 3) and quantification of protein expression from (A). Data are represented as the mean ± standard deviation (SD) of three replicates.(E) Quantification of data (in B) for VGLUT1, VGLUT2, EAAT1, and EAAT2 in week 4 neurons.(F) Table for comparisons of VGLUT and EAAT protein isoforms.(G) RT-qPCR data for SLC7A11 RNA and quantification of western blots for the encoded xCT protein in FXS and iControl NPCs and week 1 and 4 neurons. Data are represented as the mean ± SD of three replicates.(H) FMRP and Tubulin levels in FXS, iControl, and TC43-97 (FXS) NPCs. On the bottom are RT-qPCR data indicating changes in RNA levels in NPCs with varying levels of FMRP (n = 3). All data are analyzed using unpaired t tests with a Holm-Šídák multiple-comparisons test. Error bars represent the SD of three replicates. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.(I) Model depicting the changes in the Glu-Gln cycle between neurons and astrocytes based on the western blot data for the indicated protein levels (B) and (C) and metabolite levels (Figure 5C) in week 4 FXS vs. iControl neurons.