Fig 2: Wnt and BMP signaling pathways are up-regulated in Eed-deficient OPCs.(A) Heatmaps of ±3 kb around SUZ12 and H3K27ac ChIP-seq peak centers from WT OPCs. (B) Venn diagram showing the overlap between SUZ12-bound and differentially expressed genes in Eed cKO OPCs. (C) Enrichment analyses of EED targets that up-regulated in Eed-deleted OPCs. (D) GSEA plots of WNT and BMP pathway signatures. (E and F) Heatmap (E) and qRT-PCR analyses (F, n = 3 experiments) of genes associated with WNT and BMP pathways up-regulated in Eed cKO OPCs. (G) Representative SUZ12 ChIP-seq in rat OPCs and ATAC-seq tracks of indicated genes in control and Eed cKO OPCs. (H) qRT-PCR analyses of Eed in Oli-neu cells at 48 hours after transfection with siControl or siEed (n = 3 experiments). (I) ChIP-qPCR analyses for H3K27me3 enrichment at the promoters of indicated genes in Oli-neu cells treated with siControl or siEed (n = 3 experiments). (J and K) qRT-PCR analyses of myelination-associated genes in Oli-neu cells treated with siControl, siEed, or siEed together with either Wnt C59 or LDN193189 for 48 hours (n = 3 experiments). (L) Immunostaining for OLIG2 and MBP (left) or quantification of MBP+ cells (right) in control and Eed cKO OPCs treated with Wnt C59 or LDN193189 for 3 days (n > 3 experiments). Scale bars, 100 μm. Data are means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Photo credit: Jiajia Wang, CCHMC.