Fig 2: Immunostaining of RECK in renal cell tissue. RECK expression was mainly seen in the tubuli and in the capillaries of the glomeruli shown here in the medulla of non-malignant kidney tissue (A) and in the non-malignant renal cortex (B). RECK expression increased from clear cell carcinoma (C) over papillary (D) and chromophobe (E) carcinoma to oncocytoma (F). Magnification: 200× (A, B), 400× (C-F).

Fig 3: RECK expression is tumor-specific. The average staining intensity for RECK decreased to 13% in tumor tissue compared to normal tissue, whereas EMMPRIN staining remained unchanged (A). Mean RECK staining intensities for the tumor subtypes ± standard error of the mean are shown in (B). In comparison to their normal counterparts, RECK values increased gradually from 6% in clear cell carcinomas (CC) over 38% in papillary carcinomas (Pap.) and 45% in chromophobe carcinomas (Chrom.) to 96% in oncocytomas (Oncocyt.). P values are given above the pairs of columns (A, B). RECK and EMMPRIN antibodies were checked in Western blots (C). RECK bands at 125 kDa and 90 kDa were detected in protein lysates of 8 pairs of tumor and adjacent normal renal tissue (control: beta-actin). These samples were also used to show the panel of EMMPRIN bands, in our blot migrating between 35 and 74 kDa (control: beta-actin).

Fig 4: Knockdown of Rab7 significantly alleviated the symptom of CKD in vivo. (A, B) Sirius red staining was used to detect the fibrosis in kidney tissues of mice. The level of CD34 in kidney tissues of mice was detected by IHC staining. (C) The protein levels of HIF-1α, Rab7, MMP-14 and RECK in kidney tissues of mice were detected by western blot. (D–G) β-actin was used for quantification. **P<0.01.

Fig 5: Effect of RECK and MTSS1 over-expression on prostate cancer cell function.At 24 hours after transfection of either pCMV6-empty, pCMV6-RECK or pCMV6-MTSS1 into prostate cancer cells (PC-3 and DU 145), RECK and MTSS1 expression levels were verified by real time RT-PCR (A) and Western analysis (B) C. Cell viability assay, D. Invasion assay, E. Wound healing assay, Error bars represent±S.D. (standard deviation).