Hydroxy-HIF-1alpha (Pro564) (D43B5) XP ® Rabbit mAb 3434S from Cell Signaling Technology

Product Specs
ItemHydroxy-HIF-1alpha (Pro564) (D43B5) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $431.00 Inquire
Catalog Number3434S
Quantity100 µl
ApplicationsW, IP, IF-IC
ReactivityH, Mk
TargetHIF1A (Pro564) hydroxylate
HostRabbit
NCBI Gene AliasesHIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
ClonalityMonoclonal
NCBI Full Gene Namehypoxia inducible factor 1 subunit alpha
Research AreaDevelopmental Biology
CloneD43B5
Molecular Weight120

Questions and Answers

How has the Hydroxy-HIF-1α (Pro564) antibody been utilized in research related to hypoxia-inducible factors?

The Hydroxy-HIF-1α (Pro564) antibody has been shown to work in applications such as immunoblotting to detect HIF-1α levels in various cellular contexts, including studies investigating the regulation of HIF-1α by prolyl hydroxylases. For instance, it has been used to validate the upregulation of HIF-1α in response to specific inhibitors of prolyl hydroxylases, which are critical in the hypoxic response pathway ().

Can this antibody be used to study the effects of metabolic conditions on HIF-1α stability?

Yes, the Hydroxy-HIF-1α (Pro564) antibody has been employed in studies examining how metabolic conditions, such as elevated glucose levels, affect HIF-1α stability. Research indicates that elevated glucose inhibits nucleotide excision repair and destabilizes HIF-1α through enhanced prolyl hydroxylase activity, which can be assessed using this antibody in immunoblotting assays ().

Is there evidence that this antibody can help in understanding the role of HIF-1α in cancer biology?

The Hydroxy-HIF-1α (Pro564) antibody has been referenced in studies exploring the role of HIF-1α in tumor biology, particularly in the context of inflammation-induced tumorigenesis. For example, it has been used to demonstrate how MYD88 signaling can lead to HIF-1α accumulation, contributing to tumor-initiating cell generation in p53-deficient cells ().

What types of cellular models have utilized this antibody for studying HIF-1α?

This antibody has been utilized in various cellular models, including human cell lines such as HeLa and HEK293 cells, to study the regulation and function of HIF-1α under different experimental conditions, including hypoxia and metabolic stress ().

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Website: Technical Support

(1) Hydrogen sulfide increases intracellular oxygen and inhibits the HIF responseJournal of Biological ChemistryMarch 1, 2026Joseph Brake, David A. Hanna, Roshan Kumar, Qianni Peng, Aaron P. Landry, Rashi Singhal, Eranthie Weerapana, Yatrik M. Shah, Ruma Banerjee1:3000 dilution), anti-HIF-2α (R&D Systems, AF2997, 1:1000 dilution), anti-HIF-1α-OH Pro-564 (CST, 3434, 1:3000 dilution), anti-MT-CO2 (Abcam, ab110258, 1:5000 dilution), anti-SQOR (Proteintech, 17256-1-AP,

(2) Integrated multi-omics analysis reveals GPD1L-mediated dysregulation of glycerophospholipid metabolism facilitates the progression of lung adenocarcinomaRespiratory ResearchFebruary 14, 2026Yifu Sun, Yueren Yan, Yudi Zhou, Yuting Chen, Chunnan Liu, Yufang Bao, Yang Zhang, Han Han, Haiquan ChenUSA), NRF2 (12,721, Cell Signaling Technology, USA), HIF1α (ab179483, abcam, USA), Hydroxy-HIF-1 alpha (3434 T, Cell Signaling Technology, USA), β-Actin (ab6276, abcam, USA), overnight at 4 ℃. And the following

(3) Seasonality affects key physiological pathways in Pontastacus leptodactylus during an Aphanomyces astaci epidemic outbreakScientific ReportsNovember 28, 2025Maria V. Alvanou, Konstantinos Feidantsis, Ioannis Georgoulis, Apostolos P. Apostolidis, Basile Michaelidis, Ioannis A. GiantsisCusabio), anti-Bcl2 (2872, Cell Signaling), anti-Bax (B-9) (2772, Cell Signaling) and anti-Hydroxy-Hif-1α (3434, Cell Signaling). Quality transfer and protein loading were assured by Ponceau stain and actin (anti-β

(4) Intestinal mucosal barrier repair and immune regulation with an AI-developed gut-restricted PHD inhibitorNature biotechnologyNovember 1, 2025Fu Y, Ding X, Zhang M, Feng C, Yan Z, Wang F, Xu J, Lin X, Ding X, Wang L, et al.protein A-conjugated acceptor beads; PerkinElmer) with anti-HIF1α hydroxy-P546 antibody (Cell Signaling, 3434) was then added to the reaction mixture for 30 min at room temperature. The reaction was quenched with

(5) Cinobufagin Inhibits Invasion and Migration of Non-Small Cell Lung Cancer via Regulating Glucose Metabolism Reprogramming in Tumor-Associated MacrophagesDrug Des Devel TherAugust 2, 2025Ying Sun, Huitong Yang, Xue Mei, Jinchan Xia, Long Feng, Jianfeng Gao, Wei Jiang, Min Jiang, Xu Hao, Yilin Feng, et al.GTX129599-S; GeneTex), HIF-1α (1:5000; Cat#: 66730-1-Ig; Proteintech), Hydroxy-HIF-1α (1:1000; Cat#: 3434T; CST), and GAPDH (1:10000; Cat#: GTX100118; GeneTex) overnight. After washing with TBST, the membranes

(6) Nuclear SUMOylation and Proteotoxic Stress Responses to Metals with Different Ligand PreferencesChemical Research in ToxicologyMay 19, 2025Giorgiana Madalina Ursu, Casey Krawic, Anatoly ZhitkovichHSC70 (Santa Cruz, sc7298), HIF1α (BD Biosciences, 610958), Pro564-hydroxylated HIF1α (Cell Signaling, 3434), fibrillarin (Cell Signaling, 2639), L7A (Cell Signaling, 2415), γ-tubulin (Sigma, T6557). Horseradish

(7) Intestinal E. coli-produced yersiniabactin promotes profibrotic macrophages in Crohn's diseaseCell host & microbeJanuary 8, 2025Ahn JH, da Silva Pedrosa M, Lopez LR, Tibbs TN, Jeyachandran JN, Vignieri EE, Rothemich A, Cumming I, Irmscher AD, Haswell CJ, et al.Cruz,374015 Rabbit monoclonal anti-Hydroxy-HIF1α (Pro564) antibody (D43B5) (1:1000 for WB),Cell Signaling Technology,3434 Rabbit polyclonal anti-CD68 antibody (1:500 for IF),Abcam,Ab213363 Mouse monoclonal anti-CD68 antibody

(8) Research note: Increased lipid accumulation within broiler preadipocytes during differentiation in vitro at atmospheric oxygen tensionPoultry scienceJanuary 1, 2025Cruvinel JM, Greene ES, Read RW, Lee K, Dridi S, Chen PR.included rabbit anti-HIF1α (1:1000; LS-C287203; LSBio, Seattle, WA), rabbit anti-hydroxy-HIF1α (1:1000; 3434; Cell Signaling Technology, Danvers, MA), and rabbit anti-glyceraldehyde 3 phosphate dehydrogenase (GAPDH)

(9) Branched-chain α-ketoacids aerobically activate HIF1α signalling in vascular cellsNature MetabolismOctober 29, 2024Wusheng Xiao, Nishith Shrimali, Niv Vigder, William M. Oldham, Clary B. Clish, Huamei He, Samantha J. Wong, Bradley M. Wertheim, Elena Arons, Marcia C. Haigis, et al.MDH2 (cat # 11908), PHD2 (cat # 3293 and 4835), pVHL (cat # 68547), hydroxylated HIF1α-Pro564 (cat # 3434), VIM (cat # 5741), human and rat phospho-BCKDH-E1α (Ser293) (cat # 40368) antibodies were purchased

(10) Inhibition of the ubiquitin-proteasome system reduces the abundance of pyruvate dehydrogenase kinase 1 in cultured myotubesJournal of Muscle Research and Cell MotilitySeptember 1, 2024Blaž Kociper, Nives Škorja Milić, Ivana Ogrizek, Katarina Miš, Sergej Pirkmajer35",CST,9662,Rb,1:1000,O/N,O/N,Bio-Rad,1706515,"1:10,000",1 h,1 h EGFR,175,CST,2232,Rb,1:1000,O/N,O/N,Bio-Rad,1706515,"1:10,000",1 h,1 h HIF-1α,120,CST,3434,Rb,1:1000,O/N,O/N,Bio-Rad,1706515,"1:8,000",1 h,1 h p-PDHE1α (Ser293),43,Abcam,ab92696,Rb,1:1000,O/N,O/N,Bio-Rad,1706515,"1:50,000",1

(11) UCHL1-dependent control of hypoxia-inducible factor transcriptional activity during liver fibrosisBioscience ReportsJune 26, 2024Amy Collins, Rebecca Scott, Caroline L. Wilson, Giuseppe Abbate, Gabrielle B. Ecclestone, Adam G. Albanese, Demi Biddles, Steven White, Jeremy French, John Moir, et al.241809, R&D systems), rodent HIF1α (#14179, Cell Signaling Technologies), hydroxy-HIF-1α (Pro564) (#3434, Cell Signaling Technologies), VHL (#68547, Cell Signaling Technologies), ubiquitin (sc-8017, Santa Cruz

(12) Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathwaysWorld Journal of GastroenterologyJune 7, 2024Zhan-Xue Zhao, Shuai Li, Lin-Xun LiuTIANGEN); primary antibodies: Anti-HIF1α (20960-1-AP; PROTEINTECH; Chicago; United States), anti-OH-HIF1α (3434T; CST; Danvers; United States), anti-phosphatidyl inositol-4,5-bisphosphate-3-kinase-p85α (PI3K-p85α;

(13) Cholesterol accumulation impairs HIF-1α-dependent immunometabolic reprogramming of LPS-stimulated macrophages by upregulating the NRF2 pathwayScientific reportsMay 15, 2024Ting KKY, Yu P, Dow R, Ibrahim H, Karim S, Polenz CK, Winer DA, Woo M, Jongstra-Bilen J, Cybulsky MI.(CST#5174), anti-NRF2 (CST#12721), anti-iNOS (Transduction Laboratories, #N32030), anti-hydroxy-HIF-1α (CST#3434), anti-KEAP1 (ThermoFisher, #PA5-99434), anti-p-PKM2 (Y105) (CST#3827S), PKM2 (CST#4053), anti-mCherry

(14) Scutellarin activates IDH1 to exert antitumor effects in hepatocellular carcinoma progressionCell Death & DiseaseApril 15, 2024Zhao Cui, Caifeng Li, Wei Liu, Mo Sun, Shiwen Deng, Junxian Cao, Hongjun Yang, Peng Chenagainst IDH1 (1:2000, 12332-1-AP, Proteintech), HIF1a (1:1000, A22041, ABclonal), OH-HIF1a (1:2000, 3434T, Cell Signaling Technology), PFKL (1:1000, A7708, ABclonal), GLUT1 (1:10,000, 81463-1-RR, Proteintech),

(15) Cancer-associated fibroblast-secreted FGF7 as an ovarian cancer progression promoterJournal of translational medicineMarch 15, 2024Feng S, Ding B, Dai Z, Yin H, Ding Y, Liu S, Zhang K, Lin H, Xiao Z, Shen Y.anti-HIF1α (20960-1-AP, Proteintech, China), anti-HIF1α-OH402 (ab72775, Abcam, USA), anti-HIF1α-OH564 (#3434, CST, USA), anti-PHD1 (12984-1-AP, Proteintech, China), anti-PHD2 (19886-1AP, Proteintech, China), and

(16) Cigarette smoke causes a bioenergetic crisis in RPE cells involving the downregulation of HIF-1α under normoxiaCell death discoveryOctober 25, 2023Henning Y, Willbrand K, Larafa S, Weißenberg G, Matschke V, Theiss C, Görtz GE, Matschke J.temperature. Primary antibodies against HIF-1α (610958, BD Biosciences, Franklin Lakes, NJ), hydroxy-HIF-1α (3434, Cell Signaling Technology, Danvers, MA), HIF-2α (NB100-122, Novus Biologicals, Littleton, CO), TOM20

(17) LINC02774 inhibits glycolysis in glioma to destabilize HIF-1α dependent on transcription factor RP58MedComm (2020)October 1, 2023Chen Y, Liu Y, Xiong J, Ouyang L, Tang M, Mao C, Li L, Xiao D, Liu S, Yang Z, et al.control. The antibodies for Western Blot: HIF‐1α (CST, #36169S, 1:1000), Hydroxylation HIF‐1α (CST, #3434T, 1:1000), PHD3 (Novus, #NB100‐139, 1:1000), GLUT1 (CST, #12939S, 1:1000), RP58 (Proteintech, #12714‐1‐AP,

(18) H(2)O(2) transit through the mitochondrial intermembrane space promotes tumor cell growth in vitro and in vivoThe Journal of biological chemistryMay 1, 2023Sabharwal SS, Dudley VJ, Landwerlin C, Schumacker PT.dilution), HIF-1α (BD Biosciences, #610958, 1:1000 dilution), Hydroxy-HIF-1α (Pro564) (Cell Signaling, #3434, 1:1000 dilution), anti-HA tag (Abcam #ab9110, 1:1000 dilution), HIF-2α (Novus Biological, #NB-100-122,

(19) Limiting Mrs2-dependent mitochondrial Mg(2+) uptake induces metabolic programming in prolonged dietary stressCell reportsMarch 28, 2023Madaris TR, Venkatesan M, Maity S, Stein MC, Vishnu N, Venkateswaran MK, Davis JG, Ramachandran K, Uthayabalan S, Allen C, et al.234111 CPT-2,Abcam,Cat# 181114 HIF-1α,"Cell Signaling, MA, USA",Cat# 14179S Hydroxy HIF-1α,Cell Signaling,Cat# 3434S MCU,Cell Signaling,Cat #D2Z3B MICU1,Cell Signaling,Cat# D4P8Q β-Actin,Santa Cruz Biotechnology,Cat#

(20) CHD1 deletion stabilizes HIF1α to promote angiogenesis and glycolysis in prostate cancerAsian journal of andrologyMarch 1, 2023Wang YZ, Qian YC, Yang WJ, Ye LH, Guo GD, Lv W, Huan MX, Feng XY, Wang K, Yang Z, et al.CHD1 (#4351), HIF1α (#14179), von Hippel–Lindau tumor suppressor (VHL; #68547), and hydroxy-HIF1α (#3434) were purchased from Cell Signaling Technology (Danvers, MA, USA). An antibody against eukaryotic translation

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Hydroxy-HIF-1alpha (Pro564) (D43B5) XP ® Rabbit mAb

Hydroxy-HIF-1alpha (Pro564) (D43B5) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Hydroxy-HIF-1alpha (Pro564) (D43B5) XP ® Rabbit mAb from Cell Signaling Technology

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