Rabbit anti-RPC2 Antibody, Affinity Purified A301-855A from Bethyl Laboratories, Inc.

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Nature communications
May 30 2022

Fig 1: Concomitant loss of POLR3G and Pol III activity modulates cellular and exosomal small RNA repertoires during THP-1 differentiation.a RT-qPCR analysis of POLR3G and POLR3GL mRNA, and snaR-A ncRNA levels following THP-1 differentiation (n = 3 biologically independent experiments). P-value = 0.00088 (POLR3G), 0.099 (POLR3GL), 0.00014 (snaR-A seq1), 0.0013 (snaR-A seq2). Significance calculated using Student two-sided t-test. b Immunoblots for Pol III subunits mapped in THP-1 cells, ±72-h PMA-induced differentiation. *Lamin B2 corresponds to POLR3A and POLR3GL. Observations representative of 2 or more independent experiments. c, d Volcano plot visualization of dynamic POLR3G (c) and POLR3GL (d) genomic occupancy ± 72-h PMA-induced differentiation. Gene class indicated by color legend. Significance calculated using edgeR two-sided exactTest function, Benjamini–Hochberg corrected p-value. e Heatmap visualization of the log2(fold change) of Pol III subunit occupancy for POLR3B, POLR1D, POLR3G, POLR3GL, and POLR3D in THP-1 cells ± 72-h PMA treatment. Heatmap is ordered by median fold change across all subunits. Inset highlights top 35 (10%) of ranked genes. f ChIP-seq track visualization for POLR3B, POLR1D, POLR3G, POLR3GL, and POLR3D in THP-1 cells before (left) and after 72-h PMA treatment (right) across a subset of SNAR-A genes encoding snaR-A ncRNA. g Heatmap visualization of dynamic small RNA profiles in THP-1 monocytes, including nascent, steady-state, nuclear, cytoplasmic, and exosomal small RNA fractions. All heatmaps are ordered by the level of nascent RNA abundance in THP-1 monocytes. Corresponding genes for all heatmaps indicated by color legend on right. h RT-qPCR analysis of POLR3G and POLR3GL mRNA, and snaR-A ncRNA levels following THP-1 differentiation and lentiviral transduction with control or POLR3G-specific CRISPR activation particles (sc-437282 and sc-417072-LAC-2) (n = 6 biologically independent experiments). P-value = 0.00012 (POLR3G), 0.00046 (POLR3GL), 5.79e-7 (snaR-A seq1), 4.77e-5 (snaR-A seq2). Significance calculated using Student two-sided t-test. Data are presented as mean ± standard error mean (SEM) from the indicated number of independent samples (a, h). Molecular weights are indicated in kDa. *P = 0.05; **P = 0.01; ***P = 0.001; ns, nonsignificant. Source Data are provided as a Source Data file.
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Nature communications
May 30 2022

Fig 2: Study workflow and combinatorial atlas of human RNA Polymerase III subunits across canonical Pol III-transcribed genes.a Illustration of experimental approach tracing the effect of developmental loss of subunit POLR3G, subunit-specific disruption of POLR3G, and cancer-associated re-establishment of POLR3G and accompanying chromatin features, which collectively identify POLR3G-driven modulation of Pol III transcription potential in proliferating cells. b Pol III subunit and transcription factor legend corresponding to genome-wide maps in panels c–k, including POLR3A/RPC1 (3A); POLR3B/RPC2 (3B); POLR1D/RPAC2 (1D); POLR3C/RPC3 (3C); POLR3G/RPC7a (3G); POLR3GL/RPC7ß (3GL); POLR3D/RPC4 (3D); POLR3E/RPC5 (3E); BRF1/TFIIIB90; TF3C1/TFIIIC220. Corresponding subcomplex indicated. c–k Example ChIP-seq read signals for each subunit/factor are shown in THP-1 monocytes across canonical Pol III-transcribed genes of varying promoter architecture, including ribosomal 5 S rRNA genes (type 1 promoter; panel c), tRNA, 7SL, vault, and snaR-A ncRNA genes (type 2 promoter, panels d–g, respectively), and Y, U6, 7SK, and RMRP ncRNA genes (type 3 promoter, panels h–k, respectively). Gene labels include Unique RNA Sequence (URS) identifiers assigned by (and connected to) the RNAcentral database. Bottom panel illustrates corresponding promoter architecture classification. l Cartoon schematic of the Pol III protein complex with emphasis on subunits mapped in this study (labeled) and the corresponding color code for genomic signal plots. Illustration serves as general reference guide inspired by previous structural reconstructions (Hoffmann et al., Nature 2015)46. m Visualization of the correlation between log2(normalized read densities) for paralogous Pol III subunits, POLR3G and POLR3GL, at Pol III complex–occupied genes in THP-1 monocytes. Source Data are provided as a Source Data file.

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Rabbit anti-RPC2 Antibody, Affinity Purified

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Bethyl Laboratories, Inc.'s Rabbit anti-RPC2 Antibody, Affinity Purified is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Precipitation, Immunohistochemistry, Immunoprecipitation, and Western Blot. The Rabbit anti-RPC2 Antibody, Affinity Purified was generated using POLR3B as the antigen and it reacts with Homo Sapiens, and Human.

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The epitope recognized by A301-855A maps to a region between residue 1083 and 1133 of human RNA polymerase III subunit C2 using the numbering given in entry NP_060552.3 (GeneID 55703)

Nature communications

Nature communications

Rabbit anti-RPC2 Antibody, Affinity Purified from Bethyl Laboratories, Inc.

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Bethyl Laboratories, Inc.

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Rabbit anti-RPC2 Antibody, Affinity Purified from Bethyl Laboratories, Inc.

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