Fig 1: MiR-1290 aggravated Sev-inhibited cellular malignant behaviors by depleting RORA. Experimental cells in this section included control, Sev, Sev + miR-1290, Sev + miR-NC, Sev + miR-1290 + pcDNA-RORA or Sev + miR-1290 + pcDNA. A The expression of miR-1290 in Sev-treated T98G and LN18 cells transfected with miR-1290 or miR-NC was detected by qPCR. B The expression of RORA in these experimental cells was detected by western blot. C and D Cell proliferation was assessed by CCK-8 assay and colony formation assay. E Cell apoptosis was monitored by flow cytometry assay. F and G Cell cycle progression monitored by flow cytometry assay. H and I Cell migration was assessed by wound healing assay. J and K Cell migration and cell invasion were assessed by transwell assay. L and M The protein levels of MMP2 and MMP9 were measured by western blot. *P < 0.05
Fig 2: CircRELN downregulation inhibited tumor growth in vivo. A and B Tumor volume and tumor weight were measured to observe tumor growth. C Tumor tissues were excised, and representative images of tumor size were shown. D and E The expression of circRELN and miR-1290 in the excised tissues was detected by qPCR. F The expression of RORA in the excised tissues was detected by western blot. *P < 0.05
Fig 3: RORA inhibited angiogenesis in trophoblasts. (A) Tube formation was determined by tube formation assay. (B) Concentrations of Ang-1 in the HTR-8/SVneo cells were assessed using the available ELISA kit. (C) RORA, VEGFA, and VEGFR2 proteins were determined by western blot. *p<0.01 compared to normoxia group; †p<0.01 compared to si-NC group. RORA, RAR related orphan receptor A.
Fig 4: RORA inhibited HTR-8/SVneo cells migration, invasion, and epithelial-mesenchymal transition. (A) Cells invasion and migration were detected by Transwell assay. (B) Cells migration was determined by wound healing assay. (C) E-cadherin, N-cadherin, and vimentin protein were determined by western blot. *p<0.01 signifies statistically significant difference compared to normoxia group; †p<0.01 compared to si-NC group. RORA, RAR related orphan receptor A.
Fig 5: RORA binds PGC-1α promoter and promotes PGC-1α transcription. A Jaspar analyzed the relationship between RORA and PGC-1α; wild and mutant sequences of the binding site in PGC-1α promoter were synthesized for dual-luciferase reporter assay. B qRT-PCR and Western blotting were used to detect PGC-1α mRNA and protein in HK-2 cells before and after LPS treatment. Dual-luciferase reporter assay (C) and chromatin immunoprecipitation assay (D) were performed to verify the binding of RORA to PGC-1α promoter. E qRT-PCR and Western blotting were used to analyze the effect of OE-RORA transfection on the expression of PGC-1α mRNA and protein in LPS-treated HK-2 cells. Data were expressed as mean ± standard deviation and each experiment was repeated 3 times. **P < 0.01, compared with control group. #P < 0.05 and ###P < 0.001, compared with LPS + OE-NC group. &&&P < 0.001, compared with IgG group. RORA, retinoic acid receptor-related orphan receptor alpha; PGC-1α, peroxisome proliferator-activated receptor gamma coactivator 1 alpha; qRT-PCR, quantitative real-time polymerase chain reaction; LPS, lipopolysaccharide; OE-RORA, RORA overexpression vector; OE-NC, negative control vector
Supplier Page from Abcam for Anti-ROR alpha/RORA antibody