CTCF (D31H2) XP ® Rabbit mAb 3418S from Cell Signaling Technology

Product Specs
ItemCTCF (D31H2) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number3418S
Quantity100 µl
ApplicationsW, IP, IHC-P, IF-F, IF-IC, ChIP, ChIP-seq, C&R, C&T
ReactivityH, M, R, Mk
TargetCTCF
HostRabbit
ClonalityMonoclonal
NCBI Full Gene NameCCCTC-binding factor
Research AreaChromatin Regulation / Nuclear Function
IsotypeIgG
CloneD31H2
NCBI Gene AliasesCFAP108, FAP108, MRD21
Molecular Weight140

Questions and Answers

Can this antibody be used for ChIP-seq experiments?

Yes, the CTCF (D31H2) XP® Rabbit mAb has been shown to be effective in ChIP-seq applications, as evidenced by its use in studies that investigate chromatin architecture and gene regulation, including the mapping of CTCF binding sites in various cellular contexts.

What species can I use this antibody with?

The CTCF antibody is reactive with several species, including human, mouse, rat, and non-human primates, making it versatile for studies involving these organisms.

Is there any evidence of this antibody being used in cancer research?

Yes, the CTCF antibody has been cited in research related to cancer, such as studies examining the role of CTCF in T cell acute lymphoblastic leukemia, where alterations in CTCF binding were linked to changes in oncogene expression and chromatin organization.

How does this antibody perform in immunofluorescence applications?

The CTCF antibody has been utilized in immunofluorescence applications, allowing researchers to visualize CTCF localization within cells, which is crucial for understanding its role in chromatin structure and gene regulation.

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) ADNP regulates chromatin architecture and lineage fidelity during neural differentiationPLoS GenetApril 1, 2026Phillip Wulfridge, Nathaniel Rell, John Doherty, Kuo-Chen Fang, Michelle Lee Lynskey, Kavitha Sarmastored in 70% ethanol at 4°C. For immunofluorescence experiments, ADNP or CTCF antibody (Cell Signaling 3418S) was diluted in blocking solution and each incubation was followed by three washes with PBST (0.1% Tween

(2) Mutant KRAS Heterogeneity Shapes Nuclear Architecture During Pancreatic Cancer InitiationEpigenomesMarch 10, 2026Gareth Pollin, Angela J. Mathison, Elise N. Leverence, Thiago Milech De Assuncao, Juan Iovanna, Johnny C. Hong, Michael T. Zimmermann, Raul Urrutia, Gwen Lomberkstaining, nuclei were co-stained with anti-CTCF (D31H2) XP (Cell Signaling Technology, Danvers, MA, USA, 3418S). Secondary antibodies included Abberior STAR RED goat anti-rabbit IgG (STRED-1002-500UG) and Abberior

(3) Image-based epigenetic profiling with deep learning and high-speed super-resolution microscopyEpigenetics & ChromatinFebruary 28, 2026Yicheng Wang, Nur Syatila Ab Ghani, Munmee Dutta, Shungo Adachi, Kaoru Katoh, Masakazu Namihira, Toutai Mitsuyama, Yutaka Saitomethod: primary antibodies were anti-H3K27ac IgG (308–34843, MAB Institute, Inc.) and anti-CTCF IgG (3418 S, Cell Signaling Technology); the secondary antibodies were CF488 donkey anti-rabbit IgG (H + L), highly

(4) CTCF/cohesin-binding sites are susceptible to replication-associated DNA damage and genomic instability in cancer cellsiScienceFebruary 20, 2026Elangoli Ebrahimkutty Faseela, Dimple Notani, Radhakrishnan Sabarinathan(Sigma, #T8787) in 1% BSA (Invitrogen, #AM2616) at RT for 15 minutes. Next, primary antibodies (CTCF: 3418 CST 1:600, MRE11: ab214 Abcam 1:600, PCNA: ab29 Abcam 1:500, RAD21: ab992 Abcam 1:500, STN1: sc-376450

(5) Notch interaction with RUNX factors regulates initiation of the T-lineage programJ Exp MedFebruary 2, 2026Yuichi Kama, Ken-Ichi Hirano, Kaori Masuhara, Yusuke Endo, Yuka Suzuki, Masanori Fujimoto, Tatsuma Matsuda, Takashi Yahata, Masahiko Kato, Katsuto Hozumi, et al.anti-FLAG (F3165; Sigma-Aldrich, RRID:AB_259529), anti-Cbfb (62184; CST, RRID:AB_2722525), anti-CTCF (3418; CST, RRID:AB_2086791), anti-Med12 (A300-774A; Bethyl, Boston, RRID:AB_669756), and anti-Notch1-IC (4147;

(6) Clostridioides difficile TcdB induces expression of its receptor (CSPG4) through a noncanonical Hippo signaling mechanismJournal of Biological ChemistryFebruary 1, 2026Jason L. Larabee, Elizabeth J. Donald, Anushka A. Sukhadia, Tyler M. Shadid, Sarah J. Miller, Jimmy D. Ballard(Cell Signaling Technology; product # 4970); a rabbit monoclonal antibody against CTCF (Cell Signaling Technology; product # 3418); a rabbit monoclonal against LATS1 (Cell Signaling Technology; product #3477) a rabbit monoclonal against

(7) ERM proteins support perinuclear actin rim formationFrontiers in Cell and Developmental BiologyJanuary 21, 2026Yuval Hadad, Andrea Fracchia, Dagmawit Babele, Amit Ben Shushan, Gabi Gerlitzin SDS-PAGE and analyzed by Western blot analysis using the following antibodies: rabbit anti-CTCF (3418, Cell Signaling Technology, Danvers, MA, United States) diluted 1:1,000, rabbit anti-Emerin (sc-15378,

(8) Af-CUT&Tag: a sensitive and antibody-free chromatin profiling method using genetically encoded tags and high-affinity binders fused to Tn5Nature CommunicationsJanuary 17, 2026Xindong Wang, Xusheng Deng, Lu Qiu, Junjia Liu, Hengxiang Shen, Haoran Du, Weijie Li, Linxuan Song, Wenhui Deng, Xiaoning Dong, et al.AC005), Phospho-Rpb1 CTD (Ser2) (Cell Signaling Technology, 13499), CTCF (Cell Signaling Technology, 3418), YAP1 (Cell Signaling Technology, 14074), TAZ (Cell Signaling Technology, 72804), HiBiT (Promega, N7200),

(9) Enhancer-mediated NR2F2 recruitment activates BGN to promote tumor growth and shape tumor microenvironment in papillary thyroid cancerTheranosticsJanuary 1, 2026Mei Tao, Xianhui Ruan, Jialong Yu, Qiman Dong, Wei Luo, Wei Zhang, Mengran Tian, Xiukun Hou, Linfei Hu, Jingzhu Zhao, et al.USA), BRD4 antibody (Cat#13440, CST, USA), Flag antibody (Cat#14793, CST, USA), and CTCF antibody (Cat#3418S, CST, USA) followed by immunoprecipitation with magnetic beads. The samples were washed with a series

(10) Stress-induced loss of CTCF reveals an alternative, promoter-based mode of cohesin loopingbioRxivDecember 24, 2025J P Flores, Andrea A. Perreault, Zack Drum, Chenxi Xu, Doris Cruz Cruz Alonso, Gelila Petros, Yijia Wu, Ivana Y. Quiroga-Barber, HyunAh Kim, Isha Sahasrabudhe, et al.1:50 and included anti-GFP to target eGFP-YAP1 (Abcam, #ab290), anti-CTCF (Cell Signaling Technology, #3418S), anti-RAD21 (Abcam, #ab217678), and anti-H3K27ac (Abcam, #ab4729). After washing, cells were incubated

(11) The 3D Genome Structure of a Blcap-Linked Silencer Loop Regulates Terminal Differentiation During Lens Fiber Cell DenucleationInvest Ophthalmol Vis SciDecember 8, 2025Shurui Ke, Zhong Liu, Jingyu Ma, Yingfeng Zheng, Lixia Luoused included anti-H3K27me3 (no. 9733S; Cell Signaling Technology, Danvers, MA, USA), anti-CTCF (no. 3418S; Cell Signaling Technology), anti-SMC3 (no. 703926; Invitrogen), and the secondary antibody Goat Anti-Rabbit IgG (H+L

(12) Ginkgolic Acid Inhibits VSMC Proliferation and Migration and Vascular Restenosis by Regulating Cell Cycle Progression and Cytoskeleton Rearrangement Through TCTN1CellsDecember 3, 2025Yuting Shao, Lingyan Yi, Qingyu Zhu, Yulin Zhou, Tingting Chen, Wenjuan YaoAiFang Biotechnology (Changsha, China). Antibodies against β-tubulin (AT0003) and CTCF (D31H2) XP® (#3418) were purchased from CMCTAG Inc. (San Diego, CA, USA) and Cell Signaling Technology (Beverly, MA, USA),

(13) Disentangling the architectural and non-architectural functions of CTCF and cohesin in gene regulationNature GeneticsNovember 18, 2025Takeo Narita, Sinan Kilic, Yoshiki Higashijima, Natalie M. Scherer, Georgios Pappas, Elina Maskey, Chunaram Choudharyfiltered). Then, cells were incubated with anti-CTCF (1:1,000, Cell Signaling Technology, cat. no. 3418) and anti-ALFA-tag (1:500, SYSY Antibodies, cat. no. N1583) antibodies in an antibody diluent for 2

(14) Jpx RNA controls Xist induction through spatial reorganization of the mouse X-inactivation centerDevelopmental CellNovember 3, 2025Hyun Jung Oh, Priyojit Das, Roy Blum, Andrea J. Kriz, Hun-Goo Lee, Yong-Woo Lee, Jeannie T. Leenormal rabbit IgG (Cell Signaling Technology, 2729S). For western blot analysis, CTCF (Cell Signaling Technology, 3418S) and Lamin B1 (Abcam, ab16048) were used. ChIP Cells were cross-linked with 1% formaldehyde in medium

(15) MYOD represses gene expression from non-E-box motifsGenes & DevelopmentNovember 3, 2025Chiara Nicoletti, Jimmy Massenet, Andreas P. Pintado-Urbanc, Leah J. Connor, Monica Nicolau, Swetha Sundar, Mingzhi Xu, Anthony Schmitt, Wenxin Zhang, Zesen Fang, et al.h at 4°C with Protein A/G Dynabeads. In parallel, 10 µL of CTCF antibody (Cell Signaling Technology 3418S) or 1 µg of rabbit IgG (Santa Cruz Biotechnology sc-2027) was prebound to Protein A/G Dynabeads in 500

(16) Mapping of chromatin architecture and enhancer-promoter interactions in the cochleaFront Mol BiosciOctober 15, 2025Tuba Ege, Celia R. Bloom, Mi Zhou, Huizhan Liu, Litao Taousing CUT&RUN (Skene and Henikoff, 2017) with antibodies against CTCF (Cell Signaling Technology Cat# 3418, RRID:AB_2086791) to map architectural protein binding sites, H3K27ac (Active Motif Cat# 39133, RRID:AB_2561016)

(17) MUC1-C auto-regulatory complex with EBNA1 is responsible for latent Epstein-Barr virus-associated gastric cancer progressionOncogeneOctober 7, 2025Hiroki Ozawa, Yin Wang, Henry G. Withers, Naoki Haratake, Ayako Nakashoji, Atrayee Bhattacharya, Atsushi Fushimi, Chie Kikutake, Kazuhiro Yamanoi, Shaowen White, et al.dilution; Proteintech, Rosemont, IL, USA), anti-E-Cadherin (3195, 1:1000 dilution; CST), anti-CTCF (3418, 1:1000 dilution; CST), anti-RAD21 (ab992, 1:1000 dilution; Abcam), anti-PARP1 (9532, 1:1000; CST),

(18) TP63 mediates the generation of tumour-specific chromatin loops that underlie MYC activation in radiation-induced tumorigenesisNature CommunicationsSeptember 30, 2025Qiaowei Liu, Shuai Jiang, Xiang Xu, Kang Xu, Yawen Luo, Zongyuan Yu, Meida Xiang, Zhouyang Xu, Lijie Wang, Sujie Zhang, et al.(#67825S, 1:50, Cell Signaling Technology), STAG2 (#5882S, 1:50, Cell Signaling Technology), CTCF (#3418S, 1:50, Cell Signaling Technology), Goat Anti-Rabbit IgG H&L (#Ab207, 1:100, Vazyme). Hi-C Approximately

(19) RNA-binding proteins mediate the maturation of chromatin topology during differentiationNature Cell BiologySeptember 8, 2025Bondita Dehingia, Małgorzata Milewska-Puchała, Marcin Janowski, Mahmoud-Reza Rafiee, Misbah Abbas, Aleksandra Piotrowska, Jan Senge, Piotr Blaut, Dietrich Walsh, Jacqueline Severino, et al.immunoprecipitation with the CTCF antibody (10 μg per chromatin extract, anti-CTCF (D31H2) XP Rabbit mAb #3418, Cell Signaling Technology), chromatin fragments were captured on Protein-A beads, and DNA was biotinylated

(20) DNA methylation insulates genic regions from CTCF loops near nuclear speckleseLifeSeptember 3, 2025Shelby A Roseman, Allison P Siegenfeld, Ceejay Lee, Nicholas Z Lue, Amanda L Waterbury, Brian B Liausample overnight at 4°C with rotation with 5 µL of antibody (Cell Signaling Technology D31H2 anti-CTCF, 3418S). 50 µL of Protein G Dynabeads (Thermo Fisher Scientific, 10004D) were added to each sample and incubated

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CTCF (D31H2) XP ® Rabbit mAb

CTCF (D31H2) XP ® Rabbit mAb from Cell Signaling Technology

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Questions and Answers

Cell Signaling Technology

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CTCF (D31H2) XP ® Rabbit mAb from Cell Signaling Technology

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