Hexokinase I (C35C4) Rabbit mAb 2024S from Cell Signaling Technology

Product Specs
ItemHexokinase I (C35C4) Rabbit mAb
CompanyCell Signaling Technology
Price $336.00 Inquire
Catalog Number2024S
Quantity100 µl
ApplicationsW, IP, IHC-P, IF-F, IF-IC, FC-FP
ReactivityH, M
TargetHexokinase 1
IsotypeIgG
HostRabbit
NCBI Gene AliasesHK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, NEDVIBA, NMSR, RP79, hexokinase
NCBI Full Gene Namehexokinase 1
CloneC35C4
Molecular Weight102
ClonalityMonoclonal
Research AreaMetabolism

Questions and Answers

Can this antibody be used to study hexokinase 1 in mouse models?

Yes, the Hexokinase I (C35C4) Rabbit mAb has been shown to detect endogenous levels of total hexokinase I protein in both human and mouse tissues, making it suitable for studies involving mouse models.

What applications has this antibody been successfully utilized in according to the literature?

The Hexokinase I antibody has been successfully utilized in various applications including Western blotting, immunocytochemistry, immunofluorescence, and immunohistochemistry, as evidenced by its use in studies such as those by Bowman et al. (2017) and De Jesus et al. (2022).

Is there any evidence of this antibody being used in metabolic studies?

Yes, the Hexokinase I antibody has been referenced in studies focusing on glucose metabolism and metabolic reprogramming, such as in the work by De Jesus et al. (2022), which discusses the role of hexokinase 1 in regulating glucose metabolism and its implications in inflammatory responses.

How does the antibody perform in detecting hexokinase 1 protein levels in different experimental conditions?

The antibody has been used in various experimental conditions, including those involving hormonal treatments, as shown in the study by Bowman et al. (2017), where it was employed to analyze hexokinase 1 levels in uterine tissues following estradiol and progesterone treatments.

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(1) Red blood cells serve as a primary glucose sink to improve glucose tolerance at altitudeCell MetabFebruary 19, 2026Yolanda Martí-Mateos, Zohreh Safari, Shaun Bevers, Ayush D. Midha, Will R. Flanigan, Tej Joshi, Helen Huynh, Brandon R. Desousa, Skyler Y. Blume, Alan H. Baik, et al.21829–1-AP); GLUT4 (Invitrogen PA5–23052); GAPDH (Cell Signaling 2118S); Hexokinase I (Cell Signaling 2024S)) at 1:100 in 3% BSA in PBS on a rotating mixer overnight at 4C. RBCs were then washed 3X with PBS and

(2) Irisin targets the HK1–glycolysis–NLRP3 pyroptosis axis to prevent chronic kidney disease-associated vascular calcificationRenal FailureJanuary 8, 2026Weijia Xu, Peiwen Wang, Xiaowen Zhu, Zongli Zhang, Xingtong Dong, Ran An, Qi Pang, Aihua Zhangwith the following primary antibodies: anti-HK1 (1:1000; Cell Signaling Technology, Danvers, MA, USA, 2024S), PKM (1:1000; Cell Signaling Technology, Danvers, MA, USA, 4053), anti-NLRP3 (1:500; Abcam, Waltham,

(3) Irisin targets the HK1–glycolysis–NLRP3 pyroptosis axis to prevent chronic kidney disease-associated vascular calcificationRen FailJanuary 7, 2026Weijia Xu, Peiwen Wang, Xiaowen Zhu, Zongli Zhang, Xingtong Dong, Ran An, Qi Pang, Aihua Zhangwith the following primary antibodies: anti-HK1 (1:1000; Cell Signaling Technology, Danvers, MA, USA, 2024S), PKM (1:1000; Cell Signaling Technology, Danvers, MA, USA, 4053), anti-NLRP3 (1:500; Abcam, Waltham,

(4) Metabolic dysfunction promoted by mitochondrial DNA mutation burden drives retinal degenerationbioRxivAugust 1, 2025Johnathon Johnathon, Jiang Jiang, Stephanie A Stephanie A., Moorthy Moorthy, Vera L. Bonilhaantibodies: Total OXPHOS antibody cocktail (Abcam, Mouse, ab110413), HK I (Cell Signaling, Rabbit, 2024S), HK II (Cell Signaling, Rabbit, 2867S), PKM2 (Cell Signaling, Rabbit, 4053S), LDHA (Cell Signaling,

(5) Fragile X messenger ribonucleoprotein 1 (FMRP) regulates glycolytic gene expression under chronic hypoxia in HCT116 cellsSci RepApril 17, 2025Yoko Ogura, Xiaoning Sun, Zaijun Zhang, Kentaro Kawata, Jinyu Wu, Ryuma Matsubara, Atsuko Nakanishi Ozeki, Kenzui Taniue, Rena Onoguchi-Mizutani, Shungo Adachi, et al."Cell signaling technology, Cat#14179s" Rabbit monoclonal anti-HK1,IgG,1:1000,"Cell signaling technology, Cat#2024s" Rabbit monoclonal anti-PFKL,IgG,1:1000,"Abcam, Cat#ab181064" Mouse monoclonal anti-PKM1/2,IgG,1:5000,"Cell signaling technology

(6) Pro-inflammatory macrophages produce mitochondria-derived superoxide by reverse electron transport at complex I that regulates IL-1β release during NLRP3 inflammasome activationNature MetabolismFebruary 19, 2025Alva M. Casey, Dylan G. Ryan, Hiran A. Prag, Suvagata Roy Chowdhury, Eloïse Marques, Keira Turner, Anja V. Gruszczyk, Ming Yang, Dane M. Wolf, Jan Lj. Miljkovic, et al.Cleaved IL-1β (Asp117) (63124), IL-1β (12507), GAPDH (5174), gasdermin D (E9S1X) (39754), hexokinase I (2024T), NLRP3 (15101), PKM1/2 (3190), PKM2 (4053T), pyruvate dehydrogenase (3205) and VDAC (4661) antibodies

(7) Novel inhibition of central carbon metabolism pathways by Rac and Cdc42 inhibitor MBQ-167 and paclitaxelMol Cancer TherNovember 4, 2024Ailed M. Cruz-Collazo, Olga Katsara, Nilmary Grafals-Ruiz, Jessica Colon Gonzalez, Stephanie Dorta-Estremera, Victor P. Carlo, Nataliya Chorna, Robert J Schneider, Suranganie Dharmawardhanesamples with equal total protein were immunoblotted for Rac1, PAK and phosphoT423 PAK, Hexokinase I (2024S), Hexokinase II (2867S), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (2188S), total (5831S) and

(8) Novel inhibition of central carbon metabolism pathways by Rac and Cdc42 inhibitor MBQ-167 and paclitaxelMolecular Cancer TherapeuticsNovember 4, 2024Ailed M. Cruz-Collazo, Olga Katsara, Nilmary Grafals-Ruiz, Jessica Colon Colon Gonzalez, Stephanie Dorta-Estremera, Victor P. Carlo, Nataliya Chorna, Robert J. Schneider, Suranganie Dharmawardhanesamples with equal total protein were immunoblotted for Rac1, PAK and phosphoT423 PAK, Hexokinase I (2024S), Hexokinase II (2867S), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (2188S), total (5831S) and

(9) PI3K-dependent reprogramming of hexokinase isoforms controls glucose metabolism and functional responses of B lymphocytesiScienceOctober 18, 2024Paradoski BT, Hou S, Mejia EM, Olayinka-Adefemi F, Fowke D, Hatch GM, Saleem A, Banerji V, Hay N, Zeng H, et al.(cat.#: MIT1000). Primary antibodies used include the following: HK1 (Cell Signaling Technology; cat.# 2024S), HK2 (Cell Signaling Technology; cat.#: 2867S), HK3 (ThermoFisher Scientific, cat.#: PA5-84306), β-actin

(10) Targeted degradation of hexokinase 2 for anti‑inflammatory treatment in acute lung injuryMol Med RepMarch 19, 2024Jiayan Yang, Liangliang Dong, Yifan Wang, Lifen Gong, Hongwei Gao, Yicheng XieAnti-β-actin (1:1,000; cat. no. 3700; Cell Signaling Technology, Inc.), anti-HK1 (1:1,000; cat. no. 2024S; Cell Signaling Technology, Inc.) and anti-HK2 (1:1,000; cat. no. ab209874; Abcam). Subsequently, the

(11) STAT5 promotes PD-L1 expression by facilitating histone lactylation to drive immunosuppression in acute myeloid leukemiaSignal Transduction and Targeted TherapySeptember 30, 2023Ze-Wei Huang, Xue-Ning Zhang, Ling Zhang, Ling-Ling Liu, Jing-Wen Zhang, Yu-Xiang Sun, Jue-Qiong Xu, Quentin Liu, Zi-Jie Longantibodies [STAT5 (Santa Cruz, sc-1081), p-STAT5 (Cell Signaling Technology, CST, 9314 s), HK1 (CST, 2024t), AKT (CST, 4685 s), p-AKT (CST, 4058 s), PFKP (CST, 8164t), PDHA (CST, 3205t), β-actin (Santa Cruz,

(12) Integrated landscape of cardiac metabolism in end-stage human nonischemic dilated cardiomyopathyNature Cardiovascular ResearchFebruary 13, 2023Emily Flam, Cholsoon Jang, Danielle Murashige, Yifan Yang, Michael P. Morley, Sunhee Jung, Daniel S. Kantner, Hannah Pepper, Kenneth C. Bedi, Jeff Brandimarto, et al.catalog no. ab97445), ME3 (Abcam, catalog no. ab172972), HK1 (Cell Signaling Technology, catalog no. 2024S), HK2 (Cell Signaling Technology, catalog no. 2867S), MPC1 (Cell Signaling Technology, catalog no. 14462S),

(13) Identification of metabolic pathways underlying FGF1 and CHIR99021-mediated cardioprotectioniScienceJune 17, 2022Xu B, Li F, Zhang W, Su Y, Tang L, Li P, Joshi J, Yang A, Li D, Wang Z, et al.Dehydrogenase,Cell signaling technology,"3205T, RRID: AB_2162926" Hexokinase I (C35C4),Cell signaling technology,"2024T, RRID: AB_2116996" Hexokinase II (C64G5),Cell signaling technology,"2867T, RRID: AB_2232946" PKM1

(14) Hexokinase 1 cellular localization regulates the metabolic fate of glucoseMolecular cellApril 7, 2022De Jesus A, Keyhani-Nejad F, Pusec CM, Goodman L, Geier JA, Stoolman JS, Stanczyk PJ, Nguyen T, Xu K, Suresh KV, et al.Antibody,Proteintech,55259-1-AP; RRID:AB_10837225 Hexokinase I (C35C4) Rabbit mAb,Cell Signaling Technologies,2024S; RRID:AB_2116996 Hexokinase II (C64G5) Rabbit mAb,Cell Signaling Technologies,2867; RRID:AB_2232946 IL-1β

(15) Oxidative stress induced by hydrogen peroxide promotes glycolysis by activating CaMKK/LKB1/AMPK pathway in broiler breast musclePoultry ScienceMarch 1, 2022Zuodong Chen, Tong Xing, Jiaolong Li, Lin Zhang, Yun Jiang, Feng Gao(PVDF) membrane, incubated with 5% bovine serum albumin and photographed. The antibodies for HK1 (#2024S), PK (#3106S), p-AMPKαThr172 (#2535) and horseradish-peroxidase-conjugated (#7074) were purchased from

(16) Melatonin Protects Chronic Kidney Disease Mesenchymal Stem/Stromal Cells against Accumulation of Methylglyoxal via Modulation of Hexokinase-2 ExpressionBiomolecules & therapeuticsJanuary 1, 2022Go G, Yoon YM, Yoon S, Lee G, Lim JH, Han SY, Lee SH.milk for 1 h at room temperature and incubated with the appropriate primary antibodies against HK1 (#2024S, Cell Signaling Technology, Beverly, MA, USA), HK2 (#2867S, Cell Signaling Technology), VDAC1 (NB100-695,

(17) Temporal proteomics during neurogenesis reveals large-scale proteome and organelle remodeling via selective autophagyMolecular cellDecember 16, 2021Ordureau A, Kraus F, Zhang J, An H, Park S, Ahfeldt T, Paulo JA, Harper JW.RRID:AB_2858191 Anti-HSP90,Santa-Cruz,Cat#sc-69703;RRID:AB_2121191 Anti-HK1,Cell Signaling Technology,Cat#2024S;RRID:AB_2116996 Anti-PKM1,Cell Signaling Technology,Cat#7067T;RRID:AB_2715534 Anti-PKM2,Cell Signaling Technology

(18) Cardiac-specific deletion of voltage dependent anion channel 2 leads to dilated cardiomyopathy by altering calcium homeostasisNature communicationsJuly 28, 2021Shankar TS, Ramadurai DKA, Steinhorst K, Sommakia S, Badolia R, Thodou Krokidi A, Calder D, Navankasattusas S, Sander P, Kwon OS, et al.,Cell Signaling Technology Lactate dehydrogenase (LDH),1:1000,3582S,Cell Signaling Technology Hexokinase 1 (HK1),1:500,2024S,Cell Signaling Technology Calsequestrin 2 (CASQ2),1:500,ab3516,Abcam Citrate synthase (CS),1:1000,14309S,Cell Signaling Technology

(19) CDK7 Inhibitor THZ1 Induces the Cell Apoptosis of B-Cell Acute Lymphocytic Leukemia by Perturbing Cellular MetabolismFrontiers in OncologyApril 6, 2021Tuersunayi Abudureheman et al.(1:1,000; Biotime); caspase 3 (ab13847, 1:1,000; Abcam); cleaved caspase 3 (#9661, 1:1,000; CST); HK1 (#2024T, 1:1,000; CST); LDHA (#3582, 1:1,000; CST); c-MYC (#5605, 1:1,000; CST, Danvers, MA, USA); Flag (0912-1,

(20) N ‐acetylaspartate availability is essential for juvenile survival on fat‐free diet and determines metabolic healthThe FASEB JournalDecember 1, 2019Dina C. Hofer et al.(1:1000 in 5% BSA/PBST, 44995S; Cell Signaling Technology), anti-hexokinase 1 (1:1000 in 5% BSA/PBST, 2024S; Cell Signaling Technology), anti-acetylated lysine (1:1000 in 5% BSA/PBST, 9681S; Cell Signaling Technology),

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Hexokinase I (C35C4) Rabbit mAb

Hexokinase I (C35C4) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Hexokinase I (C35C4) Rabbit mAb from Cell Signaling Technology

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