Rabbit anti soybean Trypsin inhibitor antibody was used to detect purified soybean Trypsin inhibitor. Samples of ~1 and 0.25 ug of purified soybean Trypsin inhibitor per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of protein contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with rabbit anti soybean Trypsin inhibitor antibody (1/5,000 overnight on 4°C). Primary antibody was detected with Dylight 488 conjugated streptavidin and Atto 425 conjugated goat anti rabbit secondary antibody. Predicted/observed size: 24 kDa, 17 kDa for Trypsin. Other band (s): none.