Biotin anti-mouse CD3 100243 from BioLegend

Product Specs
ItemBiotin anti-mouse CD3
CompanyBioLegend
Price $47.00Supplier Page View Company Product Page
Catalog Number100243
Quantity50 µg
ApplicationsFC - Quality tested, IHC-F - Verified
Concentration0.5mg/ml
Conjugate/TagBIOTIN
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ReactivityMouse
IsotypeRat IgG2b, kappa
Clone17A2
HostRat
Antibody TypePrimary
ImmunogenγδTCR-positive T-T hybridoma D1
NCBI Full Gene NameCD3 antigen, gamma polypeptide
NCBI Gene AliasesCtg-3, Ctg3, T3g
ClonalityMonoclonal
TargetCD3

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Epigenetic profiling of hematopoietic stem cells from male mice identifies KDR and PU.1 as regulators of aging transcriptome and caloric restriction responseNature CommunicationsFebruary 20, 2026Le Zong, Bongsoo Park, Ferda Tekin-Turhan, Wakako Kuribayashi, Mayuri Tanaka-Yano, Keefer Li, Isabel Beerman(Cat# 116204, 1:100 dilution), Biotin anti-B220 (Cat# 103204, 1:100 dilution), Biotin anti-CD3 (Cat# 100244, 1:100 dilution), Biotin anti-Mac-1 (Cat# 101204, 1:100 dilution), Biotin anti-Gr-1 (Cat# 108404, 1:100

(2) Microglial epigenetic memory is associated with accelerated resolution of inflammatory pain induced by prophylactic macrophage-derived small extracellular vesiclesbioRxivJanuary 22, 2026Xuan Luo, Jason R. Wickman, Jason T. DaCunza, Yuzhen Tian, Ahmet Sacan, Seena K. AjitLy6C (1:50; #128043, BioLegend), PerCP/Cyanine5.5 Ly6G (3:50; #127615, BioLegend), biotin CD3 (3:50; #100243, BioLegend), biotin CD19 (1:25; #115504, BioLegend), and biotin NK1.1 (1:50; #108703, BioLegend). Microglia

(3) Liver sinusoidal endothelial cells constitute a major route for hemoglobin clearanceEMBO ReportsJanuary 6, 2026Gabriela Zurawska, Zuzanna Sas, Aneta Jończy, Raghunandan Mahadeva, Patryk Slusarczyk, Marta Chwałek, Daniel Seehofer, Georg Damm, Rafał Mazgaj, Marcin Skórzyński, et al.Biolegend, #123116), anti-Ly-6G/Ly-6C (Gr-1) (Biotin, Biolegend, # 108403), anti-CD3 (Biotin, Biolegend, #100243), and anti-B220 (Biotin, Biolegend, #103204), in the dilution 1:100 for 30 min at 4 °C in the dark.

(4) Homeostatic control of energy metabolism by monocyte-derived macrophagesThe EMBO JournalNovember 17, 2025Rui Martins, Birte Blankehaus, Faouzi Braza, Miguel Mesquita, Pedro Ventura, Sumnima Singh, Sebastian Weis, Maria Pires, Sara Pagnotta, Qian Wu, et al.(Biolegend, cat. #123114), CD45-APC-e780 (Invitrogen, cat. #47045182), CD3-biotin (BioLegend, cat. #100243), CD11b-BV785 (BioLegend, cat. #101243), CD11c-BV605 (BioLegend, cat. #11733), CD19-biotin (BD, cat.

(5) S. aureus exposure during cutaneous antigen sensitization causes basophil- and interleukin-4-dependent exaggerated food anaphylaxisImmunityNovember 11, 2025Mrinmoy Das, Mohammed Alasharee, Brian Woods, Saikat Mukherjee, Shira Kim, Megan Elkins, Jacqueline Ngo, Logan Magin, Maheshwor Timilshina, Juan Manuel Leyva-Castillo, et al.108403; RRID: AB_313368 mouse NKp46,Biolegend,Cat# 137615; RRID: AB_11219387 mouse CD3,Biolegend,Cat# 100243; RRID: AB_2563946 mouse IgE,Biolegend,Cat# 406907; RRID: AB_493291 Streptavidin,Biolegend,Cat# 405229 CD45,Biolegend,Cat#

(6) OGFOD1 enables AML chemo- and nutrient stress resistance by regulating protein synthesisCell MetabolismOctober 7, 2025Christina Mayerhofer, Dan Li, Trine Kristiansen, Ernst Mayerhofer, Azeem Sharda, Giulia Schiroli, Karin Gustafsson, Lingli He, Michael Mazzola, Sam Keyes, et al.101204 Biotin-Gr-1 (Ly6G),BioLegend,Cat#: 108404 Biotin-B220,BioLegend,Cat#: 103204 Biotin-CD3,BioLegend,Cat#: 100244 Mouse Fc Block,BD Biosciences,Cat#: 553142 7-aminoactinomycin D (7AAD),BD Biosciences,Cat#: 559925 "4’,6-diamidino-2-phenylindole

(7) TIM-3 ameliorates host responses to Salmonella infection by controlling iron driven CD4+ T cell differentiation and interleukin-10 formationeBioMedicineOctober 1, 2025Christa Pfeifhofer-Obermair, Natascha Brigo, Chiara Volani, Piotr Tymoszuk, Egon Demetz, Sabine Engl, Günter Weissfor T cells (anti-CD45-FITC BioLegend Cat# 147710, RRID:AB_2563542, anti-CD3-Biotin BioLegend Cat# 100243, RRID:AB_2563946 + Streptavidin-PeCy7, anti-CD4-FITC BioLegend Cat# 100405, RRID:AB_312690, anti-TIM-3-APC

(8) Transcriptional regulation of Ligase IV by an intronic regulatory element directs thymocyte developmentGenes & ImmunitySeptember 5, 2025Matthew D. Estrada, Christopher J. Gebhardt, Mariam A. Salem, Christina N. Rau, Kruthika Sharma, Rebecca A. Glynn, Craig H. Bassing, Eugene M. Oltz, Patrick L. Collins(Biolegend: 138504), c-Kit-BUV395 (BD: 584001), CD11b-BV650 (Biolegend: 101259), CD3-Biotin (Biolegend: 100244), Ly6C-Biotin (Biolegend: 108404), Ter119-Biotin (Biolegend: 116204), StrepA-V450 (BD: 560797), Ghost-viability-V510

(9) Lymphopenia drives T cell exhaustion in immunodeficient STING gain-of-function miceEMBO Molecular MedicineAugust 13, 2025Damien Freytag, Stéphane Giorgiutti, Grégoire Hopsomer, Nadège Wadier, Sabine Depauw, Philippe Mertz, Fabrice Augé, Raphaël Carapito, Isabelle Couillin, Anne-Sophie Korganow, et al.used for LT-HSC sorting,Antibodies used for LT-HSC sorting "Biotin anti-mouse CD3, 1:50",BioLegend,100243 "Biotin anti-mouse CD4, 1:1000",BioLegend,100403 "Biotin anti-mouse CD8, 1:100",eBioscience,13-0081-82 "Biotin

(10) DOCK8 in T cells promotes Th17 and Treg cell functionality to restrain mucosal mast cells and limit susceptibility to oral anaphylaxisImmunityJuly 8, 2025Janssen E, Das M, Butts J, Alasharee M, Mukherjee S, Lozano GL, Rao C, Livingston AF, Woods B, Smith E, et al.108403; RRID: AB_313368 mouse NKp46,Biolegend,Cat# 137615; RRID: AB_11219387 mouse CD3,Biolegend,Cat# 100243; RRID: AB_2563946 mouse IgE,Biolegend,Cat# 406907; RRID: AB_493291 CD45,Biolegend,Cat# 103116; RRID:

(11) RAG suppresses group 2 innate lymphoid cellsElifeMay 6, 2025Aaron M Ver Heul, Madison Mack, Lydia Zamidar, Masato Tamari, Ting-Lin Yang, Anna M Trier, Do-Hyun Kim, Hannah Janzen-Meza, Steven J Van Dyken, Chyi-Song Hsieh, et al.(1:300) Antibody,anti-mouse CD28,BioLegend,Cat#: 102115; RRID:AB_11150408,FC (1:300) Antibody,anti-mouse CD3,BioLegend,Cat#: 100243; RRID:AB_2563946,FC (1:300) Antibody,anti-mouse CD19,BioLegend,Cat#: 115503; RRID:AB_313638,FC (1:300) Antibody,anti-mouse

(12) DNA methylation drives hematopoietic stem cell aging phenotypes after proliferative stressGeroScienceApril 1, 2025Yanai H, McNeely T, Ayyar S, Leone M, Zong L, Park B, Beerman I.116204, 1/100), B220 (BioLegend, 103204, 1/100), CD11b (BioLegend, 101204, 1/100), CD3 (BioLegend, 100244, 1/100), and Gr-1 (BioLegend, 108404, 1/100). Propidium Iodide (PI) was employed to exclude dead cells

(13) The endogenous antigen-specific CD8+ T cell repertoire is composed of unbiased and biased clonotypes with differential fate commitmentsImmunityMarch 11, 2025Leena Abdullah, Francesco E. Emiliani, Chinmay M. Vaidya, Hannah Stuart, Shawn C. Musial, Fred W. Kolling, Joshua J. Obar, Pamela C. Rosato, Margaret E. Ackerman, Li Song, et al.removed by negative selection using biotinylated lineage marker antibodies (0.5ug of anti-mouse CD3 (Cat# 100244, BioLegend), anti-mouse CD4 (Cat#100404, BioLegend), anti-mouse CD8 (Cat#100704, BioLegend), anti-mouse

(14) Microbiota translocation following intestinal barrier disruption promotes Mincle-mediated training of myeloid progenitors in the bone marrowImmunityFebruary 11, 2025Iñaki Robles-Vera, Aitor Jarit-Cabanillas, Paola Brandi, María Martínez-López, Sarai Martínez-Cano, Manuel Rodrigo-Tapias, Marcos Femenía-Muiña, Ana Redondo-Urzainqui, Vanesa Nuñez, Cristina González-Correa, et al.2.4G2",Tonbo Biosciences,Cat# 70-0161; RRID: AB_2621487 Biotin anti-mouse CD3 Antibody,Biolegend,Cat# 100244; AB_2563946 Biotin Rat Anti-Mouse CD45R/B220,BD pharmigen,Cat# 553086; AB_394615 Biotin Rat Anti-Mouse CD11b

(15) Macrophage scavenger receptor A1 promotes skeletal muscle regeneration after hindlimb ischemiaThe Journal of Biomedical ResearchJanuary 1, 2025Siying Wang, Saiya Wang, Wenhan Cai, Jie Wang, Jianan Huang, Qing Yang, Hui Bai, Bin Jiang, Jingjing Ben, Hanwen Zhang, et al.anti-SR-A1 (1∶200; Cat. #NBP1-00092, Novus Biologicals, Littleton, CO, USA), anti-CD3 (1∶100; Cat. #100244, BioLegend, San Diego, CA, USA), anti-lymphocyte antigen 6 family member G (Ly6G; 1∶100, Cat. #127602,

(16) RAG suppresses group 2 innate lymphoid cellsbioRxivAugust 20, 2024Aaron M. Ver Heul, Madison Mack, Lydia Zamidar, Masato Tamari, Ting-Lin Yang, Anna M. Trier, Do-Hyun Kim, Hannah Janzen-Meza, Steven J. Van Dyken, Chyi-Song Hsieh, et al.AB_11150783 anti-mouse CD28,BioLegend,Cat#: 102115; RRID: AB_11150408 anti-mouse CD3,BioLegend,Cat#: 100243; RRID: AB_2563946 anti-mouse CD19,BioLegend,Cat#: 115503; RRID: AB_313638 anti-mouse CD11b,BioLegend,Cat#:

(17) An attenuated lymphocytic choriomeningitis virus vector enhances tumor control in mice partly via IFN-IThe Journal of clinical investigationJune 11, 2024Chung YR, Awakoaiye B, Dangi T, Irani N, Fourati S, Penaloza-MacMaster P.anti–mouse CD3 (clone 17A2, Pacific blue, BioLegend, catalog 100214; clone 17A2, biotin, BioLegend, catalog 100244), anti–mouse Ly-6G (clone RB6-8c5, biotin, eBioscience, catalog 13-5931-85), anti–mouse NK1.1 (clone

(18) Chronic stress increases metastasis via neutrophil-mediated changes to the microenvironmentCancer cellMarch 11, 2024He XY, Gao Y, Ng D, Michalopoulou E, George S, Adrover JM, Sun L, Albrengues J, Daßler-Plenker J, Han X, et al.mice). CD3+ T cells were enriched from the spleen by incubating with CD3-Biotin antibody (BioLegend, #100244) followed by anti-Biotin Microbead (#130–094-973, Miltenyi Biotec) binding and magnetic separation.

(19) Stromal Cell Isolation from Hematopoietic OrgansJournal of Visualized ExperimentsFebruary 12, 2024Trine Kristiansen, Christina Mayerhofer, Karin Gustafsson, David T. Scadden,BD Biosciences,612802, Rat anti-mouse CD31-BV421,Biolegend,102424, Rat anti-mouse CD3-Biotin,Biolegend,100244, Rat anti-mouse CD45.2-Biotin,Biolegend,109804, Rat anti-mouse CD45-PE/Cy7,Biolegend,103114, Rat

(20) Synergistic roles of tristetraprolin family members in myeloid cells in the control of inflammationLife Science AllianceJanuary 1, 2024Brittany L Snyder, Rui Huang, Adam B Burkholder, Danielle R Donahue, Beth W Mahler, Carl D Bortner, Wi S Lai, Perry J Blackshearfollowing antibodies: CD117 (c-Kit) APC (105808; BioLegend), Sca-1 APC-Cy7 (108126; BioLegend), CD3 Biotin (100244; BioLegend), Ly-6G/Ly-6C (Gr-1) Biotin (108404; BioLegend), B220 Biotin (103204; BioLegend), TER-119

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Biotin anti-mouse CD3 from BioLegend

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Biotin anti-mouse CD3 from BioLegend

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