Trap1 Antibody: RPE

Western Blot analysis of Human, Rat Human A431 and Rat Brain Membrane cell lysates showing detection of ~75 kDa Trap1 protein using Mouse Anti-Trap1 Monoclonal Antibody, Clone 3H4-2H6 (SMC-207). Lane 1: MW ladder. Lane 2: Human lysate, A431. Lane 3: Rat lysate, Rat Brain Membrane (RBM). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody (SMC-207) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Color Development: TMB solution for 15 min at RT. Predicted/Observed Size: ~75 kDa.