GRP78 Antibody: RPE

Western Blot analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 (SMC-210). Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (SMC-210) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.

ELISA analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 (SMC-210). Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (SMC-210). Courtesy of: Cristina Bonorino, Department of Basic Health Sciences - UFCSPA, School of Medicine - UCSD.