Flow Cytometry: Insulin Antibody (SPM139) [Alexa Fluor 488] [NBP2-34738AF488] - An intracellular stain was performed on Neuro2a cells with Insulin antibody (SPM 139) NBP2-34738AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Insulin Antibody (SPM139) [Alexa Fluor® 488]
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Insulin Antibody (SPM139) [Alexa Fluor® 488]
Available conjugates: Available conjugates: PerCP, Alexa Fluor 488, DyLight 680Specificity: Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.Sizes Available: 0.1 ml