Fig 1: Effects of RJW-58 in IL-10 expression via OLF-1. A, The cluster heat map reveals the results of microarray analysis. CTSS knockdown increases the mRNA levels of the olfactory receptor family. B, Western blot analysis of OR5B3, OR5I1, and OR5M3 proteins when SIM-A9 cells were treated RJW-58 (5 µM) for 24 h. C, OLF-1 disassociated with CBP by RJW-58 for 24 h treatment in SIM-A9 cells. D, The effect of RJW-58 for 24 h on OLF-1 binding to the IL-10 promoter region in SIM-A9 cells was analyzed using ChIP. E, RJW-58 treatment facilitated IL-10 promoter activity in SIM-A9. IL-10 promoters were identified using the promoter assay. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05. The statistical significance was determined using a two-tailed Student's t-test. Data are presented as the mean ± SEM.