Western Blot: eIF3X Antibody [NB100-93306] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibody: Affinity purified rabbit anti-eIF3X antibody NB100-93306 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eIF3X was also immunoprecipitated by rabbit anti-eIF3X antibody NB100-93305, which recognizes an upstream epitope. For blotting immunoprecipitated eIF3X, the ReliaBLOT (R) Reagents and Procedures (Cat. No. WB120) were used. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).