Fig 1: ATR functions downstream of CHMP4C but upstream of CHMP4B and ANCHR in the regulation of abscission(A) Localization of CHM4PC to the midbody of late cytokinetic cells upon ATR inhibition (ETP-46464, 10 μM; 1 h).(B) Quantification of CHMP4C intensity at the midbody of late cytokinetic cells upon ATR inhibition (ETP-46464, 10 μM; 1 h),(ns, Student’s t-test).(C) Localization of ATR-T1989p to late cytokinetic midbodies upon depletion of CHMP4C.(D) Quantification of ATR-T1989p intensity at late cytokinetic midbodies upon depletion of CHMP4C. (∗p = 0.0195; Student’s t-test).(E) Percentage of cells in cytokinesis upon depletion of ATR and/or CHMP4C. At least, 400 cells were analyzed in each of 4 independent experiments (∗∗p = 0.0017; Student’s t-test).(F) Localization of ANCHR to late cytokinetic midbodies upon ATR inhibition (ETP-46464, 10 μM; 1 h).(G) Quantification of ANCHR intensity at late cytokinetic midbodies upon ATR inhibition (ETP-46464, 10 μM; 1 h). (∗p = 0.0153; Student’s t-test).(H) Localization of ATR-T1989p to late cytokinetic midbodies upon depletion of CHMP4B.(I) Quantification of ATR-T1989p intensity at late cytokinetic midbodies upon depletion of CHMP4B. (ns; Student’s t-test).(J) Localization of CHM4B to the midbody of late cytokinetic cells upon ATR inhibition (ETP-46464, 10 μM; 1 h).(K) Quantification of CHM4B intensity at the midbody of late cytokinetic cells upon ATR inhibition (ETP-46464, 10 μM; 1 h). (∗∗p = 0.0077; Student’s t-test). All dot plots represent data from three independent biological replicates, each replicate is represented by a different symbol. All data have been normalized to the control sample. Data are represented as mean ± SD of the individual replicates. All scale bars are 10 μm, all enlarged image scale bars are 2 μm. HeLa cells were used in A–K. See also Figure S5.