Fig 1: cDC1 deficient TE4 mice exhibit reduced reactive gliosis.(a) Representative immunofluorescent images of reactive microglia from four strains of the male mice at 9.5-month-old. The hippocampal sections were stained with IBA1 (Red), MHC-II (Green), and P2RY12 (Magenta). (b) Percent of the area analysis covered by IBA1 (Left), MHC-II (Middle), and P2RY12 (Right). (c) Representative immunofluorescent images of reactive astrocytes from four strains of the male mice at 9.5-month-old. The hippocampal sections were stained with Vimentin (Red), GFAP (Green), and DAPI (Blue). (d) Percent area covered by GFAP (Left) and Vimentin (Middle) staining. (e) Representative images showing the co-localization of Vimentin with GFAP from hippocampal sections from TE4WT or TE4Δ+32 mice. (f) Percentage of Vimentin staining overlapping with GFAP staining from the sections from the indicated mice. n=7 for E4WT, n=9 for E4Δ+32, n=19 for TE4WT, and n=21 for TE4 Δ+32 mice. Data are presented as mean values ± SEMs. Statistical significance was defined using two-way ANOVA with Šídák’s multiple comparisons test. P value (actual value) was indicated in the figure. Scale bar, 25μm. ****P<0.0001; NS, no significance.