Peroxidase AffiniPure™ Goat Anti-Human IgG (H+L) 109-035-003 from Jackson ImmunoResearch Laboratories, Inc.

Product Specs
ItemPeroxidase AffiniPure™ Goat Anti-Human IgG (H+L)
CompanyJackson ImmunoResearch Laboratories, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Number109-035-003
Quantity2 ml
Conjugate/TagHorseradish Peroxidase
PurityThe antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads
ReactivityHuman
TargetIgG (H+L)
HostGoat
Antibody TypeSecondary
ClonalityPolyclonal
FormatAffiniPure™

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Jackson ImmunoResearch Laboratories, Inc.

872 West Baltimore Pike

West Grove, Pennsylvania 19390

United States

Phone: 1-800-FOR-JAXN (367-5296)
Phone: 610-869-4024
Fax: 610-869-0171

Email: cuserv@jacksonimmuno.com

Company Profile

Website: www.jacksonimmuno.com

(1) Affinity-controlled capture and release of engineered monoclonal antibodies by macroporous dextran hydrogels using coiled-coil interactionsmAbsDecember 31, 2023Seyed Farzad Baniahmad, Romane Oliverio, Ines Obregon-Gomez, Alma Robert, Anne E.G. Lenferink, Elena Pazos, Nick Virgilio, Xavier Banquy, Gregory De Crescenzo, Yves DurocherEnzyme-Linked Immunosorbent Assay (ELISA) (capture antibody: Jackson ImmunoResearch #109-005-008, detection antibody: #109-035-003). Similarly, the release media collected after 1, 3, 24, 48, 72 and 96 h were analyzed by ELISA. The

(2) Epitope Mapping of BmpA and BBK32 Borrelia burgdorferi Sensu Stricto Antigens for the Design of Chimeric Proteins with Potential Diagnostic ValueACS Infectious DiseasesNovember 10, 2023Weronika Grąźlewska, Lucyna Holec-Gąsior, Karolina Sołowińska, Tomasz Chmielewski, Beata Fiecek, Marinela Contreraswashing, peroxidase-labeled antibodies directed against human IgG or IgM (Jackson ImmunoResearch, #109-035-003 and #109-035-129 Ely, UK) were added, diluted 1:32,000 and 1:16,000, respectively. 3,3′,5,5′-tetramethylbenzidine

(3) Vaccination with a replication-defective cytomegalovirus vaccine elicits a glycoprotein B-specific monoclonal antibody repertoire distinct from natural infectionnpj VaccinesOctober 10, 2023Sarah M. Valencia, Eric Rochat, Melissa J. Harnois, Maria Dennis, Helen S. Webster, Bhavna Hora, Amit Kumar, Hsuan-Yuan (Sherry) Wang, Leike Li, Daniel Freed, et al.AD-2), plates were washed twice and goat anti-human HRP-conjugated IgG (Jackson ImmunoResearch Cat.No.109–035–003) secondary Ab was diluted 1:5000 and added for 2 h. Plates were developed during a 5–10 min incubation

(4) Catalytic antibodies in arrhythmogenic cardiomyopathy patients cleave desmoglein 2 and N-cadherin and impair cardiomyocyte cohesionCellular and molecular life sciences : CMLSJuly 14, 2023Yeruva S, Stangner K, Jungwirth A, Hiermaier M, Shoykhet M, Kugelmann D, Hertl M, Egami S, Ishii N, Koga H, et al.15 min and incubated in goat anti-human-HRP conjugated secondary antibody (Jackson Immuno Research; #109-035-003) at 1:20,000 dilution in TBST for 1 h at room temperature. After incubation, the membranes were washed

(5) Imprinted SARS-CoV-2 humoral immunity induces convergent Omicron RBD evolutionNatureDecember 19, 2022Yunlong Cao, Fanchong Jian, Jing Wang, Yuanling Yu, Weiliang Song, Ayijiang Yisimayi, Jing Wang, Ran An, Xiaosu Chen, Na Zhang, et al.room temperature for 20 min. Peroxidase-conjugated AffiniPure goat anti-human IgG (H+L) (0.25 μg ml−1; 109-035-003, JACKSON) was added to plates and incubated at room temperature for 15 min. Tetramethylbenzidine (54827-17-7,

(6) BA.2.12.1, BA.4 and BA.5 escape antibodies elicited by Omicron infectionNatureAugust 18, 2022Yunlong Cao et al.temperature for 20 min. Next, Peroxidase-conjugated AffiniPure Goat Anti-Human IgG (H+L) (JACKSON, 109-035-003) was applied and incubated at room temperature for 15 min. Tetramethylbenzidine (TMB) (Solarbio, 54827-17-7)

(7) mRNA vaccine boosting enhances antibody responses against SARS-CoV-2 Omicron variant in individuals with antibody deficiency syndromesCell reports. MedicineJune 21, 2022Zimmerman O, Altman Doss AM, Kaplonek P, Liang CY, VanBlargan LA, Chen RE, Monroy JM, Wedner HJ, Kulczycki A Jr, Mantia TL, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, (HRP)-conjugated goat anti-human IgG (H + L),Jackson ImmunoResearch,Cat #109-035-003 "FRNT staining mix (SARS2-02, SARS2-11, SARS2-16, SARS2-31, SARS2-38, SARS2-57, and SARS2-71)","VanBlargan

(8) Longitudinal kinetics of RBD+ antibodies in COVID-19 recovered patients over 14 monthsPLOS PathogensJune 3, 2022Tsuf Eyran, Anna Vaisman-Mentesh, David Taussig, Yael Dror, Ligal Aizik, Aya Kigel, Shai Rosenstein, Yael Bahar, Dor Ini, Ran Tur-Kaspa, et al.and 50 μL of horseradish peroxidase (HRP) conjugated goat anti-human IgG (Jackson ImmunoResearch, #109035003) / anti-human IgM (Jackson ImmunoResearch, #109035129) / anti-human IgA (Jackson ImmunoResearch, #109035011)

(9) Identification of four novel T cell autoantigens and personal autoreactive profiles in multiple sclerosisScience advancesApril 29, 2022Bronge M, Högelin KA, Thomas OG, Ruhrmann S, Carvalho-Queiroz C, Nilsson OB, Kaiser A, Zeitelhofer M, Holmgren E, Linnerbauer M, et al.five times before developing using horseradish peroxidase–conjugated goat anti-human IgG (catalog no. 109-035-003, Jackson ImmunoResearch) for 1 hour and secondary 3,3′,5,5′-tetramethylbenzidine (Sigma-Aldrich) for

(10) Natural antibodies and CRP drive anaphylatoxin production by urate crystalsScientific reportsMarch 16, 2022Wessig AK, Hoffmeister L, Klingberg A, Alberts A, Pich A, Brand K, Witte T, Neumann K.anti-ApoB (#sc-393636; 1:200; SantaCruz Biotechnology), and anti-IgG (H + L) (peroxidase-coupled; #109-035-003; Jackson ImmunoResearch, 1:10,000). For coomassie staining, gels were incubated in PageBlue (Thermo

(11) Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodiesNatureFebruary 1, 2022Cao Y, Wang J, Jian F, Xiao T, Song W, Yisimayi A, Huang W, Li Q, Wang P, An R, et al.temperature, plates were washed and incubated with 0.08 μg ml−1 goat anti-human IgG (H+L)/HRP (Jackson, 109-035-003) for 1 h incubation at room temperature. Tetramethylbenzidine (TMB) (Solarbio) was then added, and the

(12) The Potential of Developing Pan-Coronaviral Antibodies to Spike Peptides in Convalescent COVID-19 PatientsArchivum Immunologiae et Therapiae ExperimentalisDecember 1, 2021Andrii Rabets et al.for 1 h, after that the plates were washed again. Goat anti-human IgG (H + L)-horseradish peroxidase (109-035-003, Jackson ImmunoResearch) was diluted in washing buffer (1:25,000), added to the plates, and incubated

(13) BNT162b2 mRNA vaccine elicited antibody response in blood and milk of breastfeeding womenNature CommunicationsDecember 1, 2021Michal Rosenberg-Friedman et al.1 h at RT with horseradish peroxidase (HRP) conjugated anti-human IgG)Jackson Immunoresearch, #CAT 109035003)/anti-human IgA (Jackson Immunoresearch, #CAT 109035011) (25 μl, 1:5000 ratio in 3% w/v skim milk in

(14) Placental response to maternal SARS-CoV-2 infectionScientific ReportsDecember 1, 2021Mirella Mourad et al.incubated with Peroxidase AffiniPure goat anti-human IgG (H + L) and goat anti-human IgM antibodies (cat #109-035-003 and 109-035-043, Jackson ImmunoResearch, West Grove, PA, both at 1:10,000 dilution) for 1 h at 37 °C.

(15) Competitive ELISA for a serologic test to detect dengue serotype-specific anti-NS1 IgGs using high-affinity UB-DNA aptamersScientific ReportsDecember 1, 2021Ken-ichiro Matsunaga, Michiko Kimoto, Vanessa Weixun Lim, Tun-Linn Thein, Shawn Vasoo, Yee-Sin Leo, William Sun, Ichiro HiraoStreptavidin-HRP conjugate (1 mg/mL) and Peroxidase-conjugated AffiniPure Goat Anti-Human IgG (H + L) (#109-035-003, 1 mg/mL) were obtained from Jackson ImmunoResearch. Streptavidin, Tween 20, BSA, and anti-rabbit IgG

(16) Functional differences among the spike glycoproteins of multiple emerging severe acute respiratory syndrome coronavirus 2 variants of concerniScienceNovember 19, 2021Wang Q, Nair MS, Anang S, Zhang S, Nguyen H, Huang Y, Liu L, Ho DD, Sodroski JG.RRID:AB_228370 Peroxidase AffiniPure goat anti-human IgG (H+L) antibody,Jackson ImmunoResearch,Cat# 109-035-003; RRID:AB_2337577 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, VSV-G

(17) Non-neutralizing SARS CoV-2 directed polyclonal antibodies demonstrate cross-reactivity with the HA glycans of influenza virusInternational ImmunopharmacologyOctober 1, 2021Praveenkumar Murugavelu et al.MPBS. HRP conjugated secondary antibodies (anti-Mouse IgG Fc, Code: 115–035-003, Anti-Human HRP, Code: 109–035-003, Jackson Immunoresearch) were used in 1:2000 dilutions for 1 h at RT. Following the primary and secondary

(18) Neutralizing antibodies against Epstein-Barr virus infection of B cells can protect from oral viral challenge in the rhesus macaque animal modelCell Reports MedicineJuly 20, 2021Janine Mühe, Pyone Pyone Aye, Carol Quink, Jing Ying Eng, Kathleen Engelman, Keith A. Reimann, Fred Wang,Jackson ImmunoResearch,Cat# 109-096-008; RRID: AB_2337663 HRP-conjugated anti-human IgG,Jackson ImmunoResearch,Cat# 109-035-003; RRID: AB_2337577 anti-flag antibody,Sigma,Cat# F1804 HRP-conjugated anti-dig-antibody,Jackson ImmunoResearch,Cat#

(19) Identification of a Human Anti-Alpha-Toxin Monoclonal Antibody Against Staphylococcus aureus InfectionFrontiers in MicrobiologyJuly 15, 2021Fangjie Liu, Zhangchun Guan, Yu Liu, Jingjing Li, Chenghua Liu, Yaping Gao, Yuanfang Ma, Jiannan Feng, Beifen Shen, Guang Yangincubated at 37°C for 1 h. The horseradish peroxidase/Goat anti-Human antibody (1:40,000; Jackson, 109-035-003) was used to detect the bound antibody. The YG1 binding kinetics were analyzed using a Biacore X100

(20) Rapid Generation of Coronaviral Immunity Using Recombinant Peptide Modified NanodiamondsPathogensJuly 8, 2021Rostyslav Bilyy et al.1:16,000, 1:32,000, 1:64,000, and 1:128,000. Goat antihuman IgG (H+L)-horseradish peroxidase (HRP) (109-035-003, Jackson ImmunoResearch) was diluted in washing buffer (1:25,000), added to the plates, and incubated

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(1)

12/9/2015Good IgG Antibody for ELISA Shikha SainiI checked circulating anti-AKAP4 antibodies in human serum by standard ELISA. This secondary antibody works pretty well without non-specififc background.

Peroxidase AffiniPure™ Goat Anti-Human IgG (H+L) from Jackson ImmunoResearch Laboratories, Inc.

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Jackson ImmunoResearch Laboratories, Inc.

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Peroxidase AffiniPure™ Goat Anti-Human IgG (H+L) from Jackson ImmunoResearch Laboratories, Inc.

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