Fig 2: LARP1 recruits mTORC1 to LARP1-interacting mRNPs in a manner dependent of mTORC1 activity.(A) Endogenous LARP1 co-IPs endogenous mTORC1 in HEK293T cells. LARP1 antibody 087A, but not 088A, co-IPs mTORC1. The LARP1 antibody, 087A or 088A recognizes amino acids 275–325 or 575–625 of LARP1, respectively. (B) The effect of RNAse A on the interaction of LARP1 with mTORC1 and other mRNA binding proteins (PABP1 and eIF4E). Endogenous LARP1 was IPed from the lysates treated with RNAse A. (C) The effect of RNase A on the interaction of LARP1 with the components of the initiation complex. Endogenous eIF4G1 was IPed from the lysates treated with RNAse A. (D) LARP1 interacts with mTORC1 in a manner dependent on mTORC1 activity. (E) LARP1 co-IPs mTORC1 in a growth factor/amino acid stimulation-dependent manner.DOI: http://dx.doi.org/10.7554/eLife.25237.007