Fig 2: Role of the ELLBow in AFF4 interactions with ELL1/2 in nuclear extracts.(a,b) Nuclear extracts (NE) were prepared from HEK 293T cells transfected with the indicated plasmids and subjected to immunoprecipitation (IP) with anti-FLAG (a) or anti-HA (b) agarose beads. The NE inputs and IP eluates were examined by immunoblotting for the presence of the various proteins indicated on the left. A shorter exposure of the ELL2-HA panel is also shown at the bottom of b. Uncropped versions of the blots are shown in Supplementary Fig. 5.

Fig 3: MED26 and LEC are present at RDH and snRNA genes.a, b ChIP-sequence analysis was performed using duplicate ChIP samples. Examples of ChIP-seq genome browser tracks showing the presence of MED26 at RDH genes (a), and snRNA genes (b), are depicted. c Venn diagram showing the overlap of MED26-, ELL-, and ZC3H8-occupied genes. ELL and ZC3H8 ChIP-seq datasets are from are from GEO GSE4793831. d ELL and Pol II occupancy (ChIP/Input) at RDH genes is decreased by MED26 knockdown. Ct values of each ChIP are normalized to that of the input. Each value is the mean of three independent experiments and error bars show the standard deviation. The P values for the indicated comparisons were determined by Student’s t test (*P < 0.05; **P < 0.01). n = 3 biologically independent samples. n.s., Not significant. Source data are provided as a Source Data file.