Immunoprecipitation: NUMBL Antibody [NB100-88137] - Detection of Human NUMBL1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUMBL1 antibody NB100-88137 used for IP at 3 mcg/mg lysate. NUMBL1 was also immunoprecipitated by rabbit anti-NUMBL1 antibody NB100-88136, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 3 seconds.