Fig 1: Effect of QiangGuYin (QGY) treatment on the mRNA expression of OPG, RANK, and RANKL in the femur tissue. (a–c) The mRNA expression of OPG was decreased, whereas that of RANK and RANKL was increased, after secretin knockdown. The mRNA expression of OPG was increased, whereas that of RANK and RANKL was significantly decreased, in the Sec-kno + Q group, compared to levels in the Sec-kno group. Data are expressed as the mean ± SD (n = 6). ∗P < 0.05 vs. the Con group; #P < 0.05 vs. the Sec-kno group. Groups were as follows: control group (Con), secretin overexpression group (Sec-over), secretin overexpression control group (Sec-over-Con), secretin overexpression + QGY group (Sec-over + Q), secretin knockdown group (Sec-kno), secretin knockdown control group (Sec-kno-Con), and secretin knockdown + QGY group (Sec-kno + Q).
Fig 2: Detection of expressions of relevant proteins in osteoblasts via immunofluorescence and Western blotting. LP45 promotes osteoblast differentiation by regulating histone proteases in macrophages and secreting exosomes to osteoblasts. (A) Detection of RANKL expression in osteoblasts via immunofluorescence. (B) RANKL expression in osteoblasts of each group. (C) Detection of expressions of relevant proteins in osteoblasts via Western blotting. (D) Expression levels of relevant proteins in osteoblasts. (E) CCK8 experimental results. **p < 0.05, Abbreviation: ns: no statistically significant difference.
Fig 3: Effect of QiangGuYin (QGY) treatment and secretin expression on the expression levels of RANKL, RANK, and OPG in mouse serum. (a) OPG; (b) RANKL; (c) RANK. Data are expressed as the mean ± SD (n = 6). ∗P < 0.05 vs. the Con group; #P < 0.05 vs. the Sec-kno group. Groups were as follows: control group (Con), secretin overexpression group (Sec-over), secretin overexpression control group (Sec-over-Con), secretin overexpression + QGY group (Sec-over + Q), secretin knockdown group (Sec-kno), secretin knockdown control group (Sec-kno-Con), and secretin knockdown + QGY group (Sec-kno + Q).
Fig 4: Effect of QiangGuYin (QGY) treatment on the protein expression of secretin, OPG, RANK, and RANKL in the femur tissue. (a–e) The protein expression of secretin and OPG was decreased, whereas that of RANK and RANKL was increased, after secretin knockdown. The expression of secretin and OPG was increased, whereas that of RANK and RANKL was significantly decreased in the Sec-kno + Q group, as compared to that in the Sec-kno group. Data are expressed as the mean ± SD (n = 3). ∗P < 0.05 vs. the Con group; #P < 0.05 vs. the Sec-kno group. Groups were as follows: control group (Con), secretin overexpression group (Sec-over), secretin overexpression control group (Sec-over-Con), secretin overexpression + QGY group (Sec-over + Q), secretin knockdown group (Sec-kno), secretin knockdown control group (Sec-kno-Con), and secretin knockdown + QGY group (Sec-kno + Q).
Supplier Page from Abcam for Anti-RANKL antibody