GFP (D5.1) Rabbit mAb 2956S from Cell Signaling Technology

Product Specs
ItemGFP (D5.1) Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number2956S
Quantity100 µl
ApplicationsW, W-S, IHC-P
ReactivityAll
HostRabbit
ClonalityMonoclonal
Research AreaRelated Products
TargetGFP jellyfish
IsotypeIgG
CloneD5.1
Molecular Weight27

Questions and Answers

Can this antibody be used to detect GFP in various species?

Yes, the GFP (D5.1) Rabbit mAb has been shown to detect GFP, YFP, and CFP-tagged proteins exogenously expressed in a wide range of species, including human, mouse, zebrafish, and various other mammals and non-mammalian organisms.

How has this antibody been utilized in scientific research?

The GFP (D5.1) Rabbit mAb has been cited in multiple studies, including its use in Western blotting to detect GFP expression in various experimental setups, such as in the study of Notch signaling and its regulation by cyclin-dependent kinases (), as well as in the analysis of dengue virus protease activity ().

What is the recommended dilution for using this antibody in Western blotting?

The antibody is typically used at a dilution of 1:1,000 for Western blotting, as indicated in various studies that have successfully employed it to visualize GFP-tagged proteins (, ).

Does this antibody have any limitations regarding the types of fluorescent proteins it can detect?

Yes, the GFP (D5.1) Rabbit mAb specifically detects GFP, YFP, and CFP-tagged proteins but does not detect RFP-tagged proteins, which is an important consideration when selecting an antibody for experiments involving different fluorescent tags.

Cell Signaling Technology

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Phone: 978-867-2388
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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Interaction of Plasmodium yoelii tryptophan-rich antigen 7 with CD71 on macrophage membrane regulates host inflammatory responseiScienceApril 17, 2026Yifan Sun, Zhe Chen, Chenyan Du, Yao Lei, Jian Li, Hangye Zhang, Xuan Huang, Bo Wang, Shenghuan Zuo, Zhiyue Lv, et al.antibody,Abclonal,Cat#ab18184; RRID: AB_2819175 Rabbit anti-GFP antibody,Cell Signaling Technology,Cat#2956T; RRID: AB_1196615 Rabbit anti-Flag antibody,Proteintech,Cat#20543-1-AP; RRID: AB_2572314 Rabbit anti-p65

(2) Mn2+-Mediated Antiviral Activity Through Both the cGAS-STING-IFN and ROS-Apoptosis Pathways in Porcine Alveolar Macrophage CellsVet SciApril 17, 2026Wanglong Zheng, Yajing Chang, Anjing Liu, Chenyang Zhang, Weilin Hao, Tianna Chen, Qing Lu, Zhiyu Wang, Wei Wang, Nanhua Chen, et al.antibodies against phosphorylated-TBK1 mAb (p-TBK1, 5483S), TBK1 mAb (3504S), IRF3 mAb (11904S) and GFP mAb (2956) were acquired from Cell Signaling Technology (Boston, MA, USA). The rabbit p-IRF3 (Ser385) was purchased

(3) Mutant and Wild-Type RAS Cross-talk and Stoichiometric Deficiencies Are Determinants of Sensitivity to Targeted Therapies in KRASG12R Pancreatic Ductal AdenocarcinomaCancer ResearchApril 15, 2026Mandana Kamgar, G. Aaron Hobbes, Raven Davidson, Daniel Dorbin, Juliannie Herrera, John F. Langenheim, Rachel A. Burge, Mohammed Aldakkak, Ryan D. Conrardy, Aniko Szabo, et al.cat. #WH0003845M1, RRID: AB_1842235), or rabbit anti-GFP antibody (Cell Signaling Technology, cat. #2956, RRID: AB_1196615). Bioluminescence resonance energy transfer assay Human embryonic kidney HEK293T

(4) Protocol to identify covalent inhibitors targeting RhoA Cys16STAR ProtocApril 14, 2026Tin-Yan Koo, Chloe See-Hang Ho, Hillary Yui-Yan Yip, Clive Yik-Sham Chung2117S; RRID:AB_10693922 Anti-GFP (D5.1) XP®(Dilution 1:1000 for WB),Cell Signaling Technology,Cat# 2956S; RRID:AB_1196615 Rac1/2/3 Antibody (Dilution 1:1000 for WB),Cell Signaling Technology,Cat# 2465S; RRID:AB_2176152 Cdc42

(5) Constitutive AMPK activation prevents hepatocellular carcinoma development through inhibition of HNF4α activitySci AdvApril 10, 2026Zhen Sun, Bernard Linares, Cassidy Urdiales, Fiyad Alsarmi, Boyuan Sang, Nagireddy Putluri, Jeanine L. Van Nostrand,Cell Signaling Technology,13901 β-ACTIN,Sigma-Aldrich,A5441 BrdU,Abcam,ab6326 HNF4α (for ChIP-seq analysis),Abcam,ab41898 GFP,Cell Signaling Technology,2956 Phospho-HNF4A (Ser313) Antibody (aka Ser304),Abcam,ab78356 P2-HNF4A Antibody (H6939),R&D Systems,PP-H6939-00 Diet,Source,Catalog High-fat

(6) Efficient genetic perturbation of murine sensory neurons in vivo using CRISPR/Cas9The Journal of PainApril 1, 2026Guadalupe García, Jacob B. Shapiro, Zachary T. Campbellincubated overnight at 4°C with chicken anti-GFP (1:1,000, GFP-1010, Aves Labs), rabbit anti-GFP (1:200, 2956s, Cell Signaling Technology), mouse anti-Nav1.8 (1:1,000, 75–166, NeuroMab), chicken anti-peripherin

(7) Ginsenoside Rh1 Suppresses Vesicular Stomatitis Virus Replication by Inhibiting Autophagy to Promote Immune ResponsesMicroorganismsMarch 27, 2026Hongmei Chen, Qinglu Zhao, Dingcheng Wei, Zhanying Hu, Xueliang Zhu, Rui Zhanganti–Phospho-NF-κB p65 (Ser536) rabbit mAb (3033), anti-NF-κB p65 rabbit mAb (8242), anti-GFP rabbit mAb (2956), anti-ATG16rabbit mAb (8089), and HRP-linked secondary antibodies included anti-rabbit IgG (7074) and

(8) In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and TestingbioRxivMarch 25, 2026Enrique O. Ruiz, Kayla Neyra, Diana M. Lopez, Ruo-Wen Chen, Deepta Paramasamy, Quincy Bizjak, Patrick D. Halley, Yin Wei, Marcos Sotomayor, Michael G. Poirier, et al.#927-60001) for 45 min at room temperature. The Rabbit anti-GFP monoclonal antibody (Cell Signaling, cat. #2956S; 1:1000) was added to the membrane and was placed on an orbital shaker at 4°C overnight. After five

(9) Hh and EGFR-Ras signaling promote distinct steps of tumor progression in the Drosophila follicle epitheliumNat CommunMarch 24, 2026Sari Anschütz, Hannah Müller, Andrea Schubert, Jobelle M. Peralta, Todd G. Nystul, Katja Rustrat anti-ECad (DCAD2, 1:100), mouse anti-Dlg1 (DLG1, 1:100). rabbit anti-GFP D5.1 (Cell Signaling #2956, 1:1000), rabbit anti-dpERK (Cell Signaling #4370, 1:100), rat anti-Dpn (abcam ab195173, 1:100), rabbit

(10) PHGDH is a targetable driver of PDAC progressionbioRxivMarch 17, 2026Yumi Kim, Le Jin Sun, Meredith Long, Samantha Caldwell, H. Carlo Maurer, Kenneth P. Olive, Florian Karreth, Gina M. DeNicola1:1000), Ki67 (Cell Signaling, #12202, 1:1000), PHGDH (Sigma, HPA021241, 1:1000), GFP (Cell Signaling, #2956, 1:1000), phospho-S6 [S240/244] (Cell Signaling #5364, 1:1000), and ASCT2 (Thermo 20350–1-AP, 1:100).

(11) O-GlcNAcylation regulates PPAR-driven metabolic programming in intestinal stem cellsbioRxivMarch 16, 2026Thomas Hartley Hartley McDermott, Dominic R Saiz, Yesenia Barrera Barrera Millan, Ngoc Bao Phuong Phuong Ho, Matthew Torel, Eric Uher, Caleb Aboagye, Fiona Farnsworth, Gourab Lahiri, Venkataramana Thiriveedi, et al.abcam 128568), Goat polyclonal anti-GFP (1:500, abcam 6673), Rabbit monoclonal anti-GFP (1:500, CST 2956), Rabbit monoclonal anti-OLFM4 (1:1000, CST 39141), Rabbit monoclonal anti-Cleaved Caspase 3 (1:1000,

(12) Diverse roles of SERPINE1 in regulating cellular proliferation and invasionInt J OncolMarch 13, 2026Wei Wang, Pengfei Zhao, Tian Wang, Weijing Wu, Jing Li, Qiang Huang, Jing Momoincubated with primary antibodies overnight at 4°C. Primary antibodies used were: Anti-GFP (cat. no. 2956; Cell Signaling Technology, Inc.) at 1:100 dilution, anti-p-MCM3 (Ser112; cat. no. TA2362; Abmart Pharmaceutical Technology

(13) 1,2-propanediol ameliorated radiation-induced intestinal injury in miceScientific ReportsMarch 11, 2026Jiwei Zhao, Chunan Zhao, Xing Shen, Ying Jiang, Xun Wang, Aoqiang Ji, Xuewen Zhang, Shuang Xing, Gang Sun, He Xiao, et al.secondary antibody for 1 h at 37℃. The antibodies used were as follows: Olfm4 (CST,39,141), EGFP (CST,2956S), γH2AX (Millipore, Cat.05–636-1), Caspase-3 (CST,9661), BrdU (ZSGB-BIO, ZM-0013), DyLight 488 (Abbkine,

(14) Selective Isolation of TOP3B•mRNA Covalent Intermediates Using Denaturing Oligo-dT PulldownBIO-PROTOCOLMarch 5, 2026Julia E. Warrick, Michael G. KearseF3165-1mg) 16. Rabbit anti-GFP (D5.1) primary antibody (Cell Signaling Technologies, catalog number: 2956) 17. HRP-conjugated goat anti-mouse IgG (H+L) (Invitrogen, catalog number: 31430) 18. HRP-conjugated

(15) Interaction of coronavirus E protein with BRD2 plays important regulatory roles in viral replication and induction of pro-inflammatory responseJ VirolMarch 3, 2026Shumin Li, Ding Xiang LiuPhospho-NF-κB p65 (#3033), IκBα (#4814), Phospho-IκBα (#5209), IL-6 (#12912), FLAG-Tag (#8146), GFP-Tag (#2956), myc-Tag (#2276), H3 (#4499), α-Tubulin (#3873), and β-actin (#4967) were from Cell Signaling Technology.

(16) Ageing promotes microglial accumulation of slow-degrading synaptic proteinsNatureFebruary 26, 2026Ian H. Guldner, Viktoria P. Wagner, Patricia Moran-Losada, Sophia M. Shi, Sophia W. Golub, Johannes F. Hevler, Kelly Chen, Barbara T. Meese, Ali Ghoochani, Ernst Pulido, et al.Primary antibodies were used at the following dilutions: rabbit anti-GFP (Cell Signaling Technology, 2956S) at 1:400; rabbit anti-IBA1 (Fujifilm Wako, 019-19741) at 1:2,000; guinea pig anti-NeuN (Synaptic Systems,

(17) Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particlesPLOS PathogensFebruary 23, 2026Makoto Kuroda, Peter J. HalfmannBeta actin (1:1000, ab8224, Abcam), mCherry (1:1000, 43590, Cell Signaling Technology), and GFP (1:1000, 2956, Cell Signaling Technology). Quantitative Reverse Transcription PCR (qRT-PCR) Total RNA was extracted

(18) Novel RAD50 variants lead to Nijmegen Breakage Syndrome–like disorder and unplanned recombinant human growth hormone treatment responseFront Endocrinol (Lausanne)February 19, 2026Yan Gong, MingYu Jiang, ShengNan Wu, Sheng Guo, YongFen Lyuhour at room temperature. The primary antibodies were Flag-tag (1:1000, 14793S, CST), EGFP (1:1000, 2956S, CST), and GADPH (1:1000, 5174S, ABclonal). HRP-linked goat anti-rabbit IgG (1:10000, 7074S, CST) was

(19) Spatiotemporal interplay between epithelial and mesenchymal cells drives human dentinogenesisNature CommunicationsFebruary 14, 2026Wei Wei, Chuan Wu, Jing Sun, Mengjie Han, Xiuge Gu, Hanzhang Zhou, Xiaoshan Wu, Zongshan Shen, Chunmei Zhang, Jinsong Wang, et al.19929-1-AP), LEF1 (Abcam, ab137872, Clone EPR2029Y), BMP2 (Abcam,ab214821), BMP4 (Abcam, ab39973), GFP(CST, 2956, Clone D5.1) and rabbit IgG isotype control (CST, 3900, Clone DA1E) were added. Sections were subsequently

(20) Ligand-dependent Wnt signaling promotes gastric cancer metastasis through hyaluronan expression in microenvironmentNature CommunicationsFebruary 14, 2026Yuichiro Furutani, Hiroko Oshima, Chang Pyo Hong, SeonJu Choi, Ryosuke Machi, Mizuho Nakayama, Kazuhiro Murakami, Shintaro Yagi, Yukinobu Ito, Daichi Maeda, et al.SIGMA, St. Louis, MO; 1:800), anti-E-cadherin (#AF748, R&D Systems, Minneapolis, MN; 1:100), anti-GFP (#2956, Cell Signaling, Danvers, MA; 1:500), anti-CD206 (#24595, Cell signaling, Danvers, MA; 1:200), anti-F4/80

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GFP (D5.1) Rabbit mAb

GFP (D5.1) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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GFP (D5.1) Rabbit mAb from Cell Signaling Technology

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