Pyruvate Dehydrogenase (C54G1) Rabbit mAb 3205S from Cell Signaling Technology

Product Specs
ItemPyruvate Dehydrogenase (C54G1) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number3205S
Quantity100 µl
ApplicationsW, IHC-P
ReactivityH, M, R, Mk
TargetPDHA1
HostRabbit
NCBI Gene AliasesE1alpha, PDHA, PDHAD, PDHCE1A, PHE1A
ClonalityMonoclonal
NCBI Full Gene Namepyruvate dehydrogenase E1 subunit alpha 1
Research AreaMetabolism
CloneC54G1
IsotypeIgG
Molecular Weight43

Questions and Answers

Can this antibody be used to study pyruvate dehydrogenase in cancer research?

Yes, the Pyruvate Dehydrogenase (C54G1) Rabbit mAb has been utilized in studies investigating its role in cancer metabolism. For instance, a study demonstrated that USP39 regulates pyruvate handling in non-small cell lung cancer by targeting the pyruvate dehydrogenase complex, indicating the relevance of this antibody in cancer research contexts ().

What applications has this antibody been shown to work in?

The Pyruvate Dehydrogenase (C54G1) Rabbit mAb has been shown to work in Western blotting and immunohistochemistry, as evidenced by its use in various studies to analyze protein expression levels and localization in different biological contexts, including cancer and metabolic studies (, ).

Is this antibody effective for use in human samples?

Yes, the antibody has been cited in studies involving human samples, such as its application in Western blotting to assess pyruvate dehydrogenase levels in human glioma cells and other human tissues, confirming its effectiveness for use in human research (, ).

What species can this antibody detect?

The Pyruvate Dehydrogenase (C54G1) Rabbit mAb is reactive with multiple species, including human, mouse, rat, and non-human primates, making it versatile for comparative studies across different model organisms ().

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Reversible dissociation of mitochondrial Complex V balances anabolic and energy-generating needs in canceriScienceMarch 20, 2026Huabo Wang, Jie Lu, Colin Henchy, Steven J. Mullett, Adam C. Richert, Eric S. Goetzman, Ahmet Toksoz, Leonardo Hale, Brandon B. Wang, Nelli Mnatsakanyan, et al.epitope,ThermoFisher,cat#46-07-05 Anti-ATPIF1/IF1,"Cell Signaling Technology, Inc",ca#8528 Anti-PDHA1,"Cell Signaling Technology, Inc",cat#3205;RRID:AB_2162926 Anti-GAPDH,Sigma,cat#G8795;RRID:AB_1078991 Anti-β-ACTIN,"Cell Signaling Technology,

(2) Branched-chain α-keto acids impair glucose-stimulated insulin secretion in pancreatic β-cells under diabetes by reactivating the LDHA-lactate axisNature CommunicationsMarch 2, 2026Huige Lin, Melody Yuen Man Ho, Baomin Wang, Shama Mansoori, Yumei Yang, Wen Wang, Pui Kin So, Aimin Xu, Shilun Yang, Junlei Chang, et al.(Proteintech, Cat# 16588-1-AP), and PDHα1 (total and phosphor-S293; Cell Signaling Technology, Cat# 3205 and Cat# 37115). HSP90 or \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts}

(3) Citrate clearance is a major function of aconitase 2 in the canonical TCA cycleCellFebruary 27, 2026Abigail Xie, Julia S. Brunner, Sangita Chakraborty, Angela M. Montero, Anna E. Bridgeman, Katrina I. Paras, Ruobing Cui, Maider Fagoaga-Eugui, Monika Komza, Paige K. Arnold, et al.used (at 1:1000 dilution unless otherwise indicated) were: anti-PDK1 (32702; Invitrogen), anti-PDH (3205, Cell Signaling Technology), anti-PDH-E1α (pSer293; AP1062; Sigma), anti-PDH-E1α (pSer232; AP1063; Sigma),

(4) Genistein–Butein Co-Treatment Suppresses Glycolytic Metabolism and Induces Apoptotic Signaling in PC-3 Prostate Cancer CellsCurrent Issues in Molecular BiologyFebruary 27, 2026Moon-Kyun Cho, Yeji Lee, Sang-Han Lee, Hae-Seon Nam, Yoon-Jin Leepoly(ADP-ribose) polymerase (PARP; #5625), hexokinase II (HK2 #2867), and pyruvate dehydrogenase (PDH; #3205) for 2 h at room temperature. All primary antibodies from Cell Signaling Technology were used at a dilution

(5) IDH1 mutation creates a dependency on fatty acid metabolism that underlies sensitivity to cuproptosis in acute myeloid leukemia cellsInternational Journal of Medical SciencesFebruary 26, 2026Xuening Zhang, Dayuan Zheng, Tong Chu, Dongfan Yang, Kuanyun Zhang, Shaokui Liang, Lu Yang, Wenzhe Mafollowing sources: DLAT (4A4-B6-C10) Mouse mAb (#12362), Pyruvate Dehydrogenase (C54G1) Rabbit mAb (PDHA1; #3205) were from Cell signaling technology (MA, USA). Anti-Lipoic Acid antibody (ab58724), Anti-PDHA1 (phospho S293

(6) Cross-species hepatic transcriptomics identify conserved immune-metabolic reprogramming in acute-on-chronic liver failure progressionFrontiers in ImmunologyFebruary 24, 2026Panyu Chen, Yun Song, Xiao Lin, Zhaokai Zeng, Wenxi Su, Yunyun Ren, Chen Wang, Kang He, Min Shi, Yugang WangCPT1A (Abcam, #ab128568), GLUD1 (Cell Signaling Technology, #12793), PDHA1 (Cell Signaling Technology, #3205), TREM2 (Cell Signaling Technology, #55739), and CCR2 (Affinity Biosciences, #DF2711). ATP measurement Hepatic

(7) Mild Short-Term Caloric Restriction Induces Coordinated Changes That Promote Lipid Deposition While Maintaining Thermogenesis Capacity in Interscapular Brown Adipose Tissue of Male RatsFASEB JFebruary 15, 2026Giuliana Panico, Vincenzo Migliaccio, Gabriella Pinto, Anna Iliano, Gianluca Fasciolo, Martina Coletta, Paola Venditti, Maria Moreno, Angela Amoresano, Pieter de Lange, et al.anti‐vinculin (PA5‐80216 Invitrogen, Thermofisher Scientific, Italy). Anti pyruvate dehydrogenase (PDH 3205 Cell Signaling Technology, Danvers Massachusetts, USA), anti hexokinase II (Hexokinase II 2024 Cell Signaling Technology

(8) STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondriaThe EMBO JournalFebruary 11, 2026Yolanda Orantos-Aguilera, Irene Sanchez-Lopez, Carlos Pascual-Caro, Patricia Gómez-Suaga, Estela Area-Gomez, Eulalia Pozo-Guisado, Jorge Montesinos, Francisco Javier Martin-RomeroIF)",Proteintech,20641-1-AP "Rabbit anti-pyruvate dehydrogenase α1 (1:1000 in TBS-T 0.1% + 5% BSA, for IB)",Cell Signaling Technology,3205 "Donkey anti-rabbit IgG Alexa FluorTM 594 (1:1000 in blocking buffer, for IF)",ThermoFisher Scientific,A-21207 "Goat anti-rabbit IgG HRP

(9) Targeting PFKFB3 to enhance CDK4/6 inhibitor response in ER+ breast cancerResearch SquareFebruary 10, 2026Sucheta Telang, Brian F Clem, Ariamna A Herrera Miret, Leanne Price, Susan M Dougherty, Anna Schmitz, Xinmin Yin, Xipeng Ma, Xiang Zhang, Jason Chesney, et al.(2867; Cell Signaling Technology), PFK1 (8164; Cell Signaling Technology), pyruvate dehydrogenase (PDH; 3205; Cell Signaling Technology), lactate dehydrogenase A (LDHA; 3582; Cell Signaling Technology), and β-actin

(10) Distinct immunometabolic signatures of type 1 versus type 2 diabetes in a murine model of myocardial infarctionCardiovascular DiabetologyFebruary 8, 2026Katherine R. O’Quinn, Catharyne B. Wright, Mursalin Khan, Rob W. Spitz, Nadiyeh Rouhi, Jemylle G. Morato, Patricio A. Vidal, Ana C. M. Omoto, Alex A. da Silva, Jussara M. do Carmo, et al.Blocking Buffer, Thermo Scientific), and incubated with primary antibodies against PDH (1:1000; CST #3205S), phospho-PDH (Serine 293; 1:1000; CST #31866S), phospho-Akt (serine 473; 1:1000; Cell Signaling Technologies

(11) Rapid optogenetic manipulation of autophagy reveals that the nuclear pore complex is a robust autophagy substratebioRxivFebruary 3, 2026Payel Mondal, Amanda Cyril, Louis Parham, Dana Mamriev, Igor Wierzbicki, Celina Shen, David J. Gonzalez, Maximiliano A. D'Angelo, Christina G. TowersCat# 44060), NIX (1:1000, CST, Cat# 12396), FUNDC1 (1:1000, CST, Cat# 49240), PDH (1:1000, CST, Cat# 3205), TIM50 (1:1000, Abcam, Cat# AB23938), Tax1BP1 (1:1000, CST, Cat# 5105), OPTN (1:1000, CST, Cat# 70928),

(12) Elevated adipose inflammation, but reduced hepatic triacylglycerol storage in diet-induced obese Plin4−/− miceJournal of Biological ChemistryFebruary 1, 2026Atanaska Ivanova Doncheva, Ryoko Higa, Prabhat Khanal, Martine Villemo Øksenvåg Ingebrigtsen, Yuchuan Li, Shrikant Kolan, Pratibha Kolan, Ales Kvasnicka, Ingunn Jermstad, Shaista Khan, et al.Cell Signaling Technology), rabbit anti-Phospho-eIF2α (#3398, Cell Signaling Technology), rabbit anti-Pdha1 (#3205, Cell Signaling Technology), rabbit anti-Phospho Pdha1 (#ABS-194, Milipore), rabbit anti-PERK (#3179,

(13) Metabolic Crosstalk in Triple-Negative Breast Cancer Lung Metastasis: Differential Effects of Vitamin D and E in a Co-Culture SystemCancers (Basel)January 18, 2026Balquees Kanwal, Saranya Pounraj, Rumeza Hanif, Zaklina Kovacevic(Cat.#:4567T), MPC2 (Cat.#:46141S), GLUT1 (Cat.#:73015S), HKII (Cat.#:2867S), PKM2 (Cat.#:4053T), PDH (Cat.#:3205S), LDHA (Cat.#:2012S), MCT1 (Cat.#:76508S), HIF-1α (Cat.#:36169S), and c-Myc (Cat.#:5605S) were from

(14) Schwann cell Lrp1 deletion drives trigeminal neuron sensitization and orofacial pain by modulating mitochondrial function and TRPV1/TRPA1 activityJ Headache PainDecember 22, 2025Zhiting Gong, Morgan Zhang, Naijiang Liu, Kesava Asam, Stefano Martellucci, Bradley Aouizerat, Wendy Campana, Yi Ye(Cell Signaling Technology, #31866, Rabbit, 1:1000); Total PDH (tPDH) (Cell Signaling Technology, #3205, Rabbit, 1:1000); Total OXPHOS Rodent WB Antibody Cocktail (Abcam, ab110413, Mouse, 1:1000); TRPV1 (Alomone

(15) The fate of pyruvate dictates cell growth by modulating cellular redox potentialeLifeDecember 16, 2025Ashish G Toshniwal, Geanette Lam, Alex J Bott, Ahmad A Cluntun, Rachel Skabelund, Hyuck-Jin Nam, Dona R Wisidagama, Carl S Thummel, Jared Rutterfactor1:1000 for western blot Antibody,anti-PDH (host: rabbit monoclonal),Cell Signaling Technologies,Cat# 3205,Dilution factor1:1000 for western blot Antibody,anti- p-PDH (host: rabbit monoclonal),Cell Signaling Technologies

(16) Metformin alleviates diastolic dysfunction in mice with experimental diabetic cardiomyopathyJournal of Molecular and Cellular Cardiology PlusDecember 1, 2025Jordan S.F. Chan, Seyed Amirhossein Tabatabaei Dakhili, Nadeen R. Wu, Magnus J. Stenlund, Alexa N. King, Linyue Dong, Indiresh A. Mangra-Bala, Tanin Shafaati, Sally R. Ferrari, Amanda A. Greenwell, et al.phospho-PDH serine 232 (AP1063; Millipore Sigma) and phospho-PDH serine 300 (AP1064; Millipore Sigma), PDH (3205S; Cell Signaling) primary antibodies were prepared in 1:1000 dilution in 3 % bovine serum albumin (BSA)

(17) Molecular Insights into Central Core Disease: Proteomic Signatures and Potential Therapeutic Biomarkers in RYR1 I4895T MiceInternational Journal of Molecular SciencesNovember 26, 2025Lorenza Vantaggiato, Enxhi Shaba, Federica Fiore, Daniela Rossi, Vincenzo Sorrentino, Luca Bini, Claudia Landithis study were as follows: Anti-pyruvate dehydrogenase (PDH) (Cell Signaling, Danvers, MA, USA, Cat. #3205, WB 1:1000); Anti-LDHA (Cell Signaling, Cat. #3582, WB 1:1000); Anti-LDHB (Cell Signaling, Cat. #56298,

(18) Senescent Fibroblasts Drive Melanoma Progression Through GCP ‐2 Induced CREB Phosphorylation Enhancing GlycolysisAging CellNovember 19, 2025Abhijit Basu, Vida Farsam, Karmveer Singh, Diana Crisan, Nicolai Treiber, Lars Alexander Schneider, Margit Huber, Jennifer I. Engelmeyer, Björn Schumacher, Pallab Maity, et al.10E9 were purchased from Millipore and (HexI #2024; Hex II #2867; pPFKFB2 #13064; G3PDH #2118; PDH #3205; IDH1 #8137, IDH2 #12652; OGDH #13407; GDH1/2 #12793; SDH #11998; FH #4567; Cell Signalling Technologies)

(19) PFKFB2 Is Pivotal for Metabolic Flexibility and Differential Glucose UtilizationJournal of the American Heart AssociationNovember 18, 2025Kylene M. Harold, Satoshi Matsuzaki, Atul Pranay, Jie Zhu, Anna Faakye, Kenneth M. HumphriesNB300‐524,1:500 (WB) 1:1000 (immunoprecipitation),1:2000 (WB) 1:3000 (immunoprecipitation) PDH,PDH,CST: 3205S,1:1000,1:3000 Phospho‐PDH (serine 293),Phospho‐PDH (serine 293),CST:31866S,1:1000,1:3000 PDK1,PDK1,Enzo Life Sciences

(20) PLK1-mediated PDHA1 phosphorylation drives metabolic reprogramming in lung cancerOncogeneNovember 11, 2025Jia Peng, Qiongsi Zhang, Xiongjian Rao, Derek B. Allison, Yifan Kong, Ruixin Wang, Jinghui Liu, Yanquan Zhang, Wendy Katz, Zhiguo Li, et al.Markey Cancer Center. IHC staining for PLK1 (MILLIPORE, 05–844) and PDHA1 (Cell Signaling Technology, 3205) was performed by the Biospecimen Procurement and Translational Pathology Shared Resource Facility at

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Pyruvate Dehydrogenase (C54G1) Rabbit mAb

Pyruvate Dehydrogenase (C54G1) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Pyruvate Dehydrogenase (C54G1) Rabbit mAb from Cell Signaling Technology

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