Fig 1: Western blot and quantitative RT-PCR analysis of SIBLING and MMP expression in normal and prostate cancer cell linesA. Western blot (WB) and B. quantitative (fold change) histogram show significantly higher BSP and DSPP levels in cancer (PC3, LNCap, Du145) than in normal derived prostate cells (HuPepiC, RWPE1, and WPE1-NB26). C. mRNAs of BSP, OPN, and MEPE are detectable in normal and cancer derived prostate cells with significantly high levels of OPN mRNA in HuPepiC and PC3 cell, BSP mRNA in PC3, and MEPE mRNA in LNCaP. DMP1 mRNA was undetectable in DU145 cells, and DSPP mRNA was undetectable in normal-derived prostate cell, HuPepiC and RWPE-1, and cancer-derived DU145. D. WB and quantitative histogram E. show markedly higher levels of MMP9 in PC3 and HuPepiC than in other cells examined. MMP3 levels are much higher in HuPepiC and LNCap than in other cells. There is no significant difference in MMP2 levels in both normal and cancer derived cells. F. qRT-PCR histogram shows marked levels of all three MMPs without any significant difference between normal and cancer derived cells. For WB, ß-actin was used as the normalization control, and for qRT-PCR, GAPDH was used as the endogenous control. Values are mean ± SE, n=3. Data are representative of three independent experiments.