Neomycin Phosphotransferase II (NPT II) MBS613846 from MyBioSource.com

Description

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Western Blot analysis of transfected cell lysates with anti-NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids

Product Specs
ItemNeomycin Phosphotransferase II (NPT II)
CompanyMyBioSource.com
Price $285.00Supplier Page View Company Product Page
Catalog NumberMBS613846
Quantity0.1 ml
ApplicationsELISA (EL/EIA), Western Blot (WB)
Conjugate/TagUnconjugated
FormatSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide
PurityPurified
Purified
IsotypeIgG
HostRabbit
ImmunogenRecognizes bacterial Neomycin phosphotransferase II (~32kD)
ClonalityPolyclonal
TargetNeomycin Phosphotransferase II

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Questions and Answers

How can I use the Neomycin Phosphotransferase II antibody to assess transfection efficiency in my experiments?

The Neomycin Phosphotransferase II antibody can be utilized in Western Blot analysis of transfected cell lysates. This allows for the evaluation of relative transfection efficiencies, particularly in experiments where multiple plasmids are transfected in parallel. By measuring the levels of NPT II, you can determine the effectiveness of your transfection protocols.

Is this antibody suitable for use in mammalian cell studies?

Yes, the Neomycin Phosphotransferase II antibody is relevant for mammalian cell studies as it confers resistance to G418 (Geneticin), an antibiotic commonly used in mammalian cell culture. This property makes it useful for selecting successfully transfected cells in experiments involving mammalian systems.

What type of samples can I analyze with this antibody?

This antibody is designed for use with cell lysates from transfected cells. It recognizes the bacterial Neomycin phosphotransferase II, which is approximately 32 kD in size. Therefore, it is suitable for analyzing samples from experiments where NPT II expression is expected, particularly in the context of transfection studies.

Can I use this antibody for ELISA applications?

Yes, the Neomycin Phosphotransferase II antibody is also applicable for ELISA. This versatility allows researchers to quantify NPT II levels in various experimental setups, providing additional insights into transfection efficiency and antibiotic resistance mechanisms in their studies.