Immunostaining of a hippocampus neuron with anti-GluR 2 (dilution 1 : 500, red) and anti-synaptophysin (cat. no. 101 011, dilution 1 : 500, green). Positive clusters (red) can be found on the postsynaptic neuron juxtaposed to presynaptic nerve terminals (green).

Indirect immunofluorescence labeling of a PFA fixed mouse hippocampus section (dilution 1 : 500; red). Nuclei have been visualized by DAPI staining (blue).

Immunofluorescence labeling in expanded mouse cerebellar molecular layer for GluA2 AMPA receptor subunit (green, 182 103, dilution 1 : 200) and the glutamatergic postsynaptic scaffold proteins pan-MAGUK (red, NeuroMab clone K28/86) following the magnified analysis of the proteome method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981). Scale bar is 5 µm in this tissue which was expanded ~4.6-fold in X, Y, and Z from the original size.

Courtesy: Kaylie Robinson and Ann Marie Craig, University of British Columbia, Canada.
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