Histone H3 Antibody (ChIP Formulated) 2650S from Cell Signaling Technology

Product Specs
ItemHistone H3 Antibody (ChIP Formulated)
CompanyCell Signaling Technology
Price $306.00 Inquire
Catalog Number2650S
Quantity100 µl
ApplicationsChIP
ReactivityH, M
TargetH3
HostRabbit
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A
NCBI Full Gene NameH3 clustered histone 1
ClonalityPolyclonal
Research AreaChromatin Regulation / Nuclear Function

Questions and Answers

Can this histone H3 antibody be used for chromatin immunoprecipitation assays?

Yes, the Histone H3 Antibody (ChIP Formulated) has been shown to work effectively in chromatin immunoprecipitation assays, as evidenced by its use in various studies, including those that have validated its application in ChIP protocols (see and ).

What species does this antibody react with?

The Histone H3 Antibody is reactive with both human (Homo sapiens) and mouse (Mus musculus) species, making it suitable for experiments involving these organisms.

Has this antibody been cited in any significant research studies?

Yes, the Histone H3 Antibody has been cited in 57 scientific articles, indicating its widespread use and acceptance in the research community for studies related to chromatin regulation and nuclear function.

Is this antibody specific for histone H3, or does it cross-react with other histones?

The Histone H3 Antibody is specifically designed to detect endogenous levels of total histone H3 protein and does not cross-react with other histones, ensuring specificity in experimental applications.

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Website: Technical Support

(1) Nuclear Argonaute:miRNA complexes recognize target sequences within chromatin-associated RNA and silence gene expressionNucleic Acids ResAugust 27, 2025Cristina R Hofman, Jiaxin Hu, Rut Bryl, Victor Tse, David R Corey(Sigma–Aldrich, T5201), SP1 1:5000 (Cell Signaling, #5931), and Histone H3 1:20K (Cell Signaling, #2650). After primary antibody incubation, membranes were washed 3× for 10 min at room temperature with 1×

(2) Glutamate dehydrogenase 1-dependent α-ketoglutarate promotes hepatitis B virus transcription by modulating histone methylations on the covalently closed circular DNA minichromosomeClinical and Molecular HepatologyFebruary 5, 2025Sheng-Tao Cheng, Wei-Xian Chen, Hai-Jun Deng, Xin He, Hui Zhang, Ming Tan, Hai-Bo Yu, Zhen-Zhen Zhang, Ji-Hua Ren, Min-Li Yang, et al.(24311-1-AP) was purchased from Proteintech (Chicago, USA). Rabbit anti-Flag (#14793S), rabbit anti-Histone 3 (2650S) and rabbit anti-KDM7B (#37377) were purchased from Cell Signaling Technology (Boston, MA, USA). Mouse

(3) Targeting the transcription factor YY1 is synthetic lethal with loss of the histone demethylase KDM5CEMBO ReportsOctober 21, 2024Qian Zheng, Pengfei Li, Yulong Qiang, Jiachen Fan, Yuzhu Xing, Ying Zhang, Fan Yang, Feng Li, Jie Xiong(Proteintech, 60004-1-Ig), anti-β-actin (Proteintech, 66009-1-Ig), anti-H3 (Cell Signaling Technology, 2650) and anti-H3K4me3 (Cell Signaling Technology, 9751). Coimmunoprecipitation (Co-IP) For the immunoprecipitation assay

(4) RAR-Dependent and RAR-Independent RXR Signaling in Stem-like Glioma CellsInternational Journal of Molecular SciencesNovember 17, 2023Amanda Dabrock, Natalie Ernesti, Florian Will, Manaf Rana, Nadja Leinung, Phillip Ehrich, Volker Tronnier, Christina Zechelare listed in the File S2 Section S7. The antibodies used were αIgG (#2729, Cell Signaling), αH3 (#2650, Cell Signaling), αH3K9me2/3 (#5327, Cell Signaling), and αH3K9ac (#9674, Cell Signaling). qRT-PCR analysis:

(5) Extrachromosomal Telomeres Derived from Excessive Strand DisplacementsbioRxivOctober 27, 2023Junyeop Lee, Jina Lee, Eric J. Sohn, Angelo Taglialatela, Roderick J. O’Sullivan, Alberto Ciccia, Jaewon MinCoprecipitant, Invitrogen, AM9515), anti-Beta actin (abcam, ab125096), anti-Histone H3 (Cell signaling, 2650), anti-ATRX (Santa Cruz, sc-15408), anti-hMRE-11 (Calbiochem, PC388), anti-GAPDH (Cell signaling, 97166),

(6) Nuclear IL-33 Plays an Important Role in EGFR-Mediated Keratinocyte Migration by Regulating the Activation of Signal Transducer and Activator of Transcription 3 and NF-κBJID InnovJuly 1, 2023Dai X, Shiraishi K, Muto J, Mori H, Murakami M, Sayama K.(Ser726) Antibody,3037,"Cell Signaling Technology (Danvers, MA)","Western blotting (1/1,000)" Histone H3,2650,"Cell Signaling Technology (Danvers, MA)",ChIP assay (1/400) Lamin A,613501,"BioLegend (San Diego,

(7) Non-canonical functions of SNAIL drive context-specific cancer progressionNature communicationsMarch 7, 2023Paul MC, Schneeweis C, Falcomatà C, Shan C, Rossmeisl D, Koutsouli S, Klement C, Zukowska M, Widholz SA, Jesinghaus M, et al.Technology) and rabbit IgG (1:50, #2729, C15D3, Cell Signaling Technology) as negative control and H3 (1:50, #2650, Cell Signaling Technology) as positive control. Binding of SNAIL to the DNA regions of interest was

(8) Histone macroH2A1 is a stronger regulator of hippocampal transcription and memory than macroH2A2 in miceCommunications BiologyDecember 1, 2022Gurdeep Singh et al.mH2A1(1:500, Abcam Cat#37264), mH2A2 (1:500, Active Motif Cat#39873) and H3 (1:10,000, Cell Signaling Cat#2650) overnight at 4 °C. After washing, membranes were incubated with the appropriate secondary antibody

(9) A role for the mitotic proteins Bub3 and BuGZ in transcriptional regulation of catalase-3 expressionPLOS GeneticsJune 6, 2022Yike Zhou, Shuangjie Shen, Chengcheng Du, Ying Wang, Yi Liu, Qun Heassay was carried out with 10 μL of antibody to BuGZ (self-prepared), 3 μL of antibody to H3 (CST, #2650), 2 μL of antibody to H2B (Abcam, #ab1790), 5 μL of antibody to CPC-1 (self-prepared), 8 μL of antibody

(10) Cannabinoid CB2 receptors are upregulated via bivalent histone modifications and control primary afferent input to the spinal cord in neuropathic painJournal of Biological ChemistryJune 1, 2022Krishna Ghosh, Guang-Fen Zhang, Hong Chen, Shao-Rui Chen, Hui-Lin Panimmunoprecipitation using the protein G magnetic beads and 5 μg of the following antibodies: rabbit anti-histone H3 (#2650S, Cell Signaling Technology), rabbit anti-H3K4me3 (#9751S, Cell Signaling Technology), rabbit anti-H3K9ac

(11) H3K56 deacetylation and H2A.Z deposition are required for aberrant heterochromatin spreadingNucleic Acids ResearchApril 22, 2022Chengcheng Zhang, Yuan Tian, Shuang Song, Lu Zhang, Yunkun Dang, Qun He10 μl antibody to H2A.Z, 3 μl antibody to acetylated H3K56 (ab76307; Abcam) or 3 μl antibody to H3 (2650; CST). Immunoprecipitated DNA was enriched with the GammaBind G Sepharose beads (17-0885-02; GE Healthcare)

(12) ATP-binding cassette protein ABCF1 couples transcription and genome surveillance in embryonic stem cells through low-complexity domainScience advancesOctober 29, 2021Choi EB, Vodnala M, Zerbato M, Wang J, Ho JJ, Inouye C, Ding L, Fong YW.(AB5603) from EMD Millipore; V5 ChIP grade (ab15828) from Abcam; and histone H3 (ChIP formulated, no. 2650) from Cell Signaling Technology (CST). Monoclonal antibodies against ACTB (66009-1) were purchased from

(13) CDC20 promotes bone formation via APC/C dependent ubiquitination and degradation of p65EMBO reportsSeptember 6, 2021Du Y, Zhang M, Liu X, Li Z, Hu M, Tian Y, Lv L, Zhang X, Liu Y, Zhang P, et al.HA‐Tag (Proteintech, 66006‐1‐Ig), His‐Tag (Proteintech, 66005‐1‐Ig), H3 (Cell Signaling Technology, 2650S), CDC20 (Abcam, ab183479), APC2 (Cell Signaling Technology, 12301S), APC11 (Cell Signaling Technology,

(14) Argonaute binding within human nuclear RNA and its impact on alternative splicingRNASeptember 1, 2021Yongjun Chu, Shinnichi Yokota, Jing Liu, Audrius Kilikevicius, Krystal C. Johnson, David R. Coreyanti-LaminA/C, 1:1500 (ab8984, Abcam), anti-βTubulin, 1:5000 (T5201, Sigma-Aldrich), anti-Histone H3, 1:20000 (2650S, Cell Signaling) 22 Downloaded from rnajournal.cshlp.org on November 2, 2021 - Published by Cold

(15) Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal agingCell stem cellJune 3, 2021Deng P, Yuan Q, Cheng Y, Li J, Liu Z, Liu Y, Li Y, Su T, Wang J, Salvo ME, et al.rabbit anti-H3K9me3 (1:500, Abcam, Cat#ab8898), rabbit anti-H3 (1:200, Cell Signaling Technology, Cat#2650), anti-mouse IgG (1:500 Abcam, Cat#ab197767), and anti-rabbit IgG (1:200, Cell Signaling Technology,

(16) Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic OsteosarcomaCancersMay 30, 2021Nasarre P, Garcia DI, Siegel JB, Bonilla IV, Mukherjee R, Hilliard E, Chakraborty P, Nasarre C, Yustein JT, Lang M, et al.1% Tween, 5% milk): rabbit anti-CD38 (1:1000; #14637s), rabbit anti-histone H3 antibodies (1:5000; #2650s), and β-catenin (1:1000; #9582S), all from Cell Signaling (Danvers, MA, USA); rabbit anti-FZD5 (1:1000;

(17) Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion During AgingCellular and molecular gastroenterology and hepatologyJanuary 1, 2021Hayashi Y, Asuzu DT, Bardsley MR, Gajdos GB, Kvasha SM, Linden DR, Nagy RA, Saravanaperumal SA, Syed SA, Toyomasu Y, et al.pAb,5605,IgG,,1:2000 ERK1/2,CST,Mouse mAb,3A7,IgG1,,1:4000 ETV1,Abcam,Rabbit pAb,Ab81086,GR12174-15,,0.5 μg/mL H3,CST,Rabbit pAb,2650,TF268338,,1:2000 LC3B,CST,Rabbit pAb,2775,5,,1:2000 KLF4,RDS,Goat pAb,AF3158,WPR0208121,,0.2 μg/mL KIT,Dakoe,Rabbit

(18) Deficiency of the X-inactivation escaping gene KDM5C in clear cell renal cell carcinoma promotes tumorigenicity by reprogramming glycogen metabolism and inhibiting ferroptosisTheranosticsJanuary 1, 2021Zheng Q, Li P, Zhou X, Qiang Y, Fan J, Lin Y, Chen Y, Guo J, Wang F, Xue H, et al.antibodies used are KDM5C (Bethyl, A301-034A), VHL (ABclonal, A0377), H3K4me3 (ABclonal, A2357), H3 (CST, 2650), α-Tubulin (Proteintech, 66031). RNA-seq Total RNA was extracted in triplicate from RCC4-PHAGE (EV)

(19) Loss of myocyte enhancer factor 2 expression in osteoclasts leads to opposing skeletal phenotypesBoneSeptember 1, 2020Blixt N, Norton A, Zhang A, Aparicio C, Prasad H, Gopalakrishnan R, Jensen ED, Mansky KC.Number c-Fos,Rabbit,CST,2250S,9 c-Jun,Rabbit,CST,9165S,11 C/EBPα,Rabbit,CST,8178S,3 CREB,Rabbit,CST,9197S,16 H3,Rabbit,CST,2650S,3 MEF2A,Rabbit,Abcam,227120,GR3210298–1 MEF2D,Rabbit,Abcam,32845,GR3176036–1 MITF,Rabbit,Sigma,HPA003259,B57783 NFATc1,Mouse,Santa

(20) The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cellsCommunications biologyMay 25, 2020Fatima A, Irmak D, Noormohammadi A, Rinschen MM, Das A, Leidecker O, Schindler C, Sánchez-Gaya V, Wagle P, Pokrzywa W, et al.beads (Pierce), the samples were incubated overnight with 2 μg histone H3 antibody (Cell Signaling, #2650) at 4 °C. As a negative control, the same amount of protein was incubated with 2 μg anti-FLAG antibody

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Histone H3 Antibody (ChIP Formulated)

Histone H3 Antibody (ChIP Formulated) from Cell Signaling Technology

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Cell Signaling Technology

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Histone H3 Antibody (ChIP Formulated) from Cell Signaling Technology

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