Fig 1: KCTD15 silencing causes the G2 m alteration of the SKBR3 cell cycle. (A) Flow cytometry analysis of the cell cycle distribution in SKBR3ctrl (left) and SKBR3KCTD15− (right) cells after 72 h of active growth. (B) Histogram representation of cell cycle phase percentage, derived from three independent experiments. * = p < 0.05 Mann–Whitney t-test. Error bars indicate standard deviation. (C) Western blot of p21, p27, KCTD15 and Tubulin in SKBR3ctrl (left) and SKBR3KCTD15− (right) cell extracts after 72 h of active growth. Numbers represent protein marker expressed in kDa. Western blot was repeated twice with similar results.