OPN (LFMb-14) Antibody sc-73631 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemOPN (LFMb-14) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-73631
NCBI Gene AliasesBSPI, Apl-1, ETA-1, Bsp, Eta, Ric, Spp-1, Opnl, 2AR, BNSP, OPN, Opn, OP
Antibody TypePrimary Antibodies
Quantity200 µg/ml
CloneLFMb-14
Reactivitymouse, rat, human
ApplicationsWB, IP, IF, IHC(P), FCM
FormatPurified
Hostmouse
Conjugate/TagUnconjugated
NCBI Full Gene Namesecreted phosphoprotein 1
IsotypeIgG2b
TargetOPN
ClonalityMonoclonal

Questions and Answers

What species does this antibody react with, and in what applications has it been used?

The OPN (LFMb-14) antibody reacts with mouse, human, and rat species. It has been shown to work in applications such as Western blotting, immunofluorescence, immunohistochemistry, flow cytometry, and immunoprecipitation.

Has this antibody been utilized in any specific cancer research, and what were the findings?

Yes, the OPN antibody has been used in studies related to breast cancer, where it was found that OPN expression correlated with vasculogenic mimicry and could predict the response to neoadjuvant chemotherapy in patients. Specifically, OPN-positive expression was associated with a higher likelihood of achieving a pathological complete response to treatment ().

Can this antibody be used to study osteopontin's role in other diseases?

Yes, the OPN antibody has been utilized in various studies, including those investigating its role in kidney injury and bladder cancer. For instance, in a study on kidney injury, OPN levels were found to be altered in response to calcium oxalate crystal deposition, indicating its involvement in renal pathology (). In bladder cancer, higher OPN expression was associated with advanced tumor stages and poorer survival outcomes, suggesting its potential as a prognostic marker ().

What experimental techniques have been employed with this antibody in the literature?

The OPN antibody has been employed in multiple experimental techniques, including immunohistochemistry for tissue analysis, Western blotting for protein expression studies, and immunofluorescence for cellular localization studies. For example, it was used at a dilution of 1:400 for immunohistochemical staining in breast cancer samples ().

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Thiamet-G facilitates reparative dentin formation via modulating O-GlcNAcylation and inflammationFrontiers in PhysiologyJanuary 16, 2026Elina Pokharel, Tae-Young Kim, Bandana Rana, Je-Hee Jang, Jae-Hee Lee, Seo-Young An, Chang-Hyeon An, Hitoshi Yamamoto, Mee-Seon Kim, Wern-Joo Sohn, et al.2021). For immunohistochemistry, anti-NESTIN (AB11306, Abcam), anti-TGF-β (ab92486, Abcam), anti-OPN (sc-73631, Santa Cruz Biotechnology), anti-MPO (bs-4943R, Bioss), anti-TNF-α (ab9739, Abcam), anti-NF-κB (bs-50467R,

(2) Single-cell and spatial transcriptomics reveal correlation between RNA methylation-related miRNA risk model and immune infiltration in hepatocellular carcinomaFront OncolMay 9, 2025Rong Su, Yong Du, Pan Tian, Weifang Ma, Yongfeng Hui, Shaoqi Yangwere blocked with immunofluorescence sealing fluid (Beyotime) and incubated with anti-SPP1 (1:1000, sc-73631, Santa Cruz Biotechnology) at 4°C overnight. After washing with PBS, macrophages were incubated with

(3) Acetylcholine Sustains LNCaP Prostate Cancer Cell Migration, Invasion and Proliferation Through Glyoxalase 1/MG-H1 Axis with the Involvement of OsteopontinInternational Journal of Molecular SciencesApril 25, 2025Dominga Manfredelli, Tatiana Armeni, Lidia de Bari, Andrea Scirè, Vincenzo Nicola Talesa, Cinzia Antognelli, Marilena Parianosc-133144, DBA Italia S.r.l., Milan, Italy), mouse anti-OPN mAb (dilution 1:1000, Santa Cruz, cat. sc-73631, DBA Italia S.r.l., Milan, Italy) and mouse anti-β-actin mAb (dilution 1:1000, Santa Cruz, cat. sc-517582,

(4) Brown adipose tissue-derived extracellular vesicles regulate hepatocyte mitochondrial activity to alleviate high-fat diet-induced jawbone osteoporosis in miceFront Endocrinol (Lausanne)April 24, 2025Kai Zhang, Sha Zhang, Guorong Deng, Guangxiang He, Yuan Yuan, Yu Fu, Yihan Liu, Zhen Gong, Liang Kong, Chenxi Zhengimmunofluorescence. Primary antibodies were diluted and applied as follows: for osteopontin (OPN) (1:200, sc-73631, Santa Cruz, America), runt-related transcription factor 2 (RUNX2) (1:200, #12556, Cell Signaling, America),

(5) An injectable multifunctional nanocomposite hydrogel promotes vascularized bone regeneration by regulating macrophagesJ NanobiotechnologyApril 7, 2025Huaiyuan Zhang, Yu Wang, Wenyu Qiao, Xueneng Hu, Huifen Qiang, Kuo Xia, Longhai Du, Luling Yang, Yi Bao, Jie Gao, et al.Western blotting employed 12% SDS-PAGE with anti-RUNX2 (1:1000, Abcam ab236639) and anti-OPN (1:500, Santa Cruz sc-73631) antibodies. Establishment and treatment of the femoral defect model in rats (1) Animal Model and

(6) Macrophage-derived SPP1 exacerbate myocardial injury by interacting with fibroblasts in viral myocarditisBiol DirectMarch 14, 2025Xiuyun Duan, Li Zhang, Keyu Liu, Kaiyin Guo, Yingnan You, Hailin Jia, Shan Zhou, Bo Hannonfit milk in TBST for 1 h at room temperature and incubated overnight at 4℃ with anti-SPP1 (1:1000, sc-73631, Santa Cruz, USA). The second day, membranes were further incubated with HRP-conjugated Affinipure Goat

(7) Placental Tissue Calcification and Its Molecular Pathways in Female Patients with Late-Onset PreeclampsiaBiomoleculesSeptember 30, 2024Miguel A. Ortega, Tatiana Pekarek, Diego De Leon-Oliva, Diego Liviu Boaru, Oscar Fraile-Martinez, Cielo García-Montero, Julia Bujan, Leonel Pekarek, Silvestra Barrena-Blázquez, Raquel Gragera, et al.USA","0.1% Triton in PBS, 10 min before incubation with blocking solution." OSP,Mouse,1:50,"Santa Cruz(sc-73631) Dallas, TX, USA","0.1% Triton in PBS, 10 min before incubation with blocking solution." PEDF,Mouse,1:500,"Abcam(ab115489)

(8) Immune cell infiltration and drug response in glioblastoma multiforme: insights from oxidative stress-related genesCancer Cell InternationalApril 2, 2024Kan Wang, Yifei Xiao, Ruipeng Zheng, Yu Cheng,Santa cruz,89 kDa,sc-377180,1:1000,1:200 BMP2,Santa cruz,45 kDa,sc-137087,1:1000,1:100 SPP1,Santa cruz,55 kDa,sc-73631,1:500,1:100 HADHA,Santa cruz,83 kDa,sc-374497,1:1000,1:150 SLC25A20,ABclonal,32 kDa,A23763,1:1000,1:100 TXNIP,Santa cruz

(9) A Facile Crystallization Strategy to Turn Calcium Bisphosphonates into Novel Osteogenesis-Inducing BiomaterialsAdvanced healthcare materialsSeptember 1, 2023Zhuang C, Zhu G, Wang Y, Wang L, Shi X, Mao C.antibodies for osteocalcin (OCN) ((ABOC‐5021): sc‐73464, Santa Cruz) and osteopontin (OPN) ((LFMb‐14): sc‐73631, Santa Cruz) at 1:250 dilution with PBS overnight at 4 °C. For qRT–PCR assay, after 7 and 14 days of

(10) Antiandrogen treatment induces stromal cell reprogramming to promote castration resistance in prostate cancerCancer cellJuly 10, 2023Wang H, Li N, Liu Q, Guo J, Pan Q, Cheng B, Xu J, Dong B, Yang G, Yang B, et al.

(11) Inhibition of p90RSK Ameliorates PDGF-BB-Mediated Phenotypic Change of Vascular Smooth Muscle Cell and Subsequent Hyperplasia of NeointimaInternational journal of molecular sciencesApril 30, 2023Hwang AR, Lee HJ, Kim S, Park SH, Woo CH.in this study were as follows: mouse anti-CDK4 (DCS-35, cat. no. sc-23896), mouse anti-OPN (cat. no. sc-73631), mouse anti-Ki-67 (cat. no. SC-23900), mouse anti-PCNA (cat. no. SC-56), and rabbit anti-p27 (C-19,

(12) Hydrogel armed with Bmp2 mRNA-enriched exosomes enhances bone regenerationJournal of nanobiotechnologyApril 5, 2023Yang Z, Li X, Gan X, Wei M, Wang C, Yang G, Zhao Y, Zhu Z, Wang Z.(11,308–1-AP, ProteinTech), TSG101 (ab83, Abcam), CD81 (ab286173, Abcam), Hsp70 (4872T, Cell-Signaling), OPN (sc-73631, Santa Cruz Biotechnology), COL-1 (ab260043, Abcam) and anti-GAPDH (D110016-0100, BBI Life Sciences)

(13) Spatial and single-cell transcriptomics decipher the cellular environment containing HLA-G+ cancer cells and SPP1+ macrophages in colorectal cancerCell reportsJanuary 31, 2023Ozato Y, Kojima Y, Kobayashi Y, Hisamatsu Y, Toshima T, Yonemura Y, Masuda T, Kagawa K, Goto Y, Utou M, et al.

(14) Expression of Osteopontin and Integrin αvβ3 Receptor in Retina of Diabetic Guinea Pigs with High MyopiaOphthalmic researchJanuary 1, 2023Dong J, Hu L, Li C, Zhang R, Zheng M, Wu X, Wu C.

(15) Ginsenoside Rh1 Inhibits Angiotensin II-Induced Vascular Smooth Muscle Cell Migration and Proliferation through Suppression of the ROS-Mediated ERK1/2/p90RSK/KLF4 Signaling PathwayAntioxidants (Basel, Switzerland)March 27, 2022Huynh DTN, Jin Y, Van Nguyen D, Myung CS, Heo KS.were purchased from Merck Millipore (Burlington, MA, USA). Mouse anti-PCNA (#sc-56), mouse anti-OPN (#sc-73631), and mouse anti-KLF4 (#sc-393462) antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas,

(16) Graphene-Oxide Porous Biopolymer Hybrids Enhance In Vitro Osteogenic Differentiation and Promote Ectopic Osteogenesis In VivoInternational journal of molecular sciencesJanuary 1, 2022Șelaru A, Herman H, Vlăsceanu GM, Dinescu S, Gharbia S, Baltă C, Roșu M, Mihali CV, Ioniță M, Serafim A, et al.anti-mouse RUNX2 (diluted at 1:100; sc-390715, Santa Cruz Biotechnology, CA, USA) and OPN (diluted at 1:100; sc-73631, Santa Cruz Biotechnology, CA, USA) primary antibodies. For visualization, Novocastra Peroxidase/DAB

(17) Bushen huoxue decoction inhibits RANKL-stimulated osteoclastogenesis and glucocorticoid-induced bone loss by modulating the NF-κB, ERK, and JNK signaling pathwaysFrontiers in pharmacologyJanuary 1, 2022Liu Y, Fu B, Li X, Chen C, Li X, Xu L, Wang B.Cell Signaling Technology (Boston, MA, United States). Antibodies against NFATc1 (sc-7294), p65 (sc-8008),OPN(sc-73631),ALP (sc-365765) and Ctsk (sc-376803)were purchased from Santa Cruz Biotechnology (Dallas, CA, United

(18) Black Phosphorus Nanoparticles Promote Osteogenic Differentiation of EMSCs Through Upregulated TG2 ExpressionNanoscale research lettersOctober 12, 2021Lu N, Wang X, Shi W, Bian L, Zhang X, Yang G, Tang X, Wang J, Zou Y, Weng Y.Biological Technology, Ltd.), anti-OCN(1:200; sc-390877, Santa Cruz Biotechnology, Inc.), anti-OPN (1:200; sc-73631, Santa Cruz Biotechnology, Inc.), anti-COL I (1:500; cat. no. BA0325; Wuhan Boster Biological Technology,

(19) 1,2,3-Triazole tethered 1,2,4‑trioxane trimer induces apoptosis in metastatic cancer cells and inhibits their proliferation, migration and invasionBioorganic chemistryJuly 1, 2021Khongsti K, Pasupuleti BG, Das B, Bez G.

(20) Decompression effects on bone healing in rat mandible osteomyelitisScientific reportsJune 3, 2021Sodnom-Ish B, Eo MY, Oh JH, Seo MH, Yang HJ, Lee JH, Kim SM.(TGF-β1) (sc-130348; Santa Cruz Biotechnology, Dallas, TX, US) at 1:100 dilution, Osteopontin (OPN) (sc-73631; Santa Cruz Biotechnology, Dallas, TX, US) at 1:100 dilution, Alkaline Phosphatase (ALP) (sc-271431;

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OPN (LFMb-14) Antibody

OPN (LFMb-14) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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OPN (LFMb-14) Antibody from Santa Cruz Biotechnology, Inc.

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