Fig 1: NLRP3 activation in platelets.NLRP3 activation was evaluated by visualizing the co-localization of NLRP3 and apoptosis-associated speck-like protein (ASC) using immunocytochemistry (A). Caspase 1 activity was assessed in all groups (n = 6/group, B). Platelets were isolated from Sham, CLP, and CLP+MCC950 animals and cultured in media overnight and IL-1ß secretion was measured via ELISA (C). Platelets were stimulated in vitro with LPS and cultured with or without MCC950 at varying doses (1, 5, or 10 µM). Media was collected and IL-1ß was measured in media via ELISA (D). P < 0.05 versus Sham *p<0.05 vs Sham or media only, #p<0.05 vs CLP or LPS-stimulated platelets.