Fig 1: An LND epithelial cell type expands in active Crohn’s disease.A UMAP of 13 epithelial cell types in TI. B UMAP labeled with expression of LCN2, NOS2, and DUOX2 in TI. C UMAP of 12 epithelial cell types in AC. D UMAP labeled with expression of LCN2, NOS2, and DUOX2 in AC. E Proportional changes of each epithelial cell type from controls to inactive and active CD patients in TI (two-sided Wilcoxon test with FDR adjustment). F Proportional changes of each epithelial cell from controls to inactive and active CD patients in AC (two-sided Wilcoxon test with FDR adjustment). G Proportional changes of LND cells with disease status in six independent cohorts (two-sided Wilcoxon test with FDR adjustment). H Heatmap of high expression of immune-related genes in the LND in both TI (top) and AC (bottom). I Representative HCR-FISH Co-staining of LCN2 (green), NOS2 (pink), and DUOX2 (red) on non-IBD controls, CD with low LND and CD with high LND in TI and AC tissues (N = 2–5 per group). The scale bar represents 100 μm. Data are represented as the mean ± SD (E–G).
Fig 2: Spatial organization between LND and immune cells.A H&E images for the four patient samples overlaid and colored by the expression of DUOX2. B Dotplot of colocalization of LND markes (LCN2, NOS2, and DUOX2) and the general epithelial genes (KRT8 and KRT18) with immune signatures. Only significant colocalization (FDR < 0.01) is included. Dot size denotes the z-value and color denotes the colocalization significance compared to random distribution. C Dotplot of colocalization between epiethial and immune cells. Only significant colocalization (FDR < 0.01) is included. Dot size denotes the z-value and color denotes the colocalization significance compared to random distribution.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for DUOX2 Antibody [Alexa Fluor® 647]
Available conjugates: Available conjugates: Alexa Fluor 647Sizes Available: 0.1 ml