Fig 1: Functional specialization of the fibroblast subtypes.A UMAP plot showing fibroblast subtypes. B Representative micrograph of myCAFs in the stroma of MIBC samples immunostained for COL1A1 (red), POSTN (green) and MMP11 (yellow). Nuclei were stained with DAPI (blue). Scale bar = 20 μm, merged scale bar = 10 μm. C Average cell numbers and proportions of fibroblast subtypes in tissues. D Volcano plot showing the upregulated and downregulated genes of fibroblasts in MIBC compared with NMIBC. Red: upregulated genes; blue: downregulated genes; gray: genes whose expression did not significantly change. E Dot plot showing the expression levels of collagen genes. F Violin plot showing the expression levels of hyaluronic acid synthase. Dot plot showing the expression levels of MMP (G)- and angiogenesis-associated (H)-related genes in fibroblast subtypes. I Heatmap indicating the scaled expression levels of chemokine genes in fibroblast subtypes. J Dot plot indicating the expression levels of growth factor-related genes in fibroblast subtypes. K RNA velocity analysis showing the transitions of fibroblast subtypes.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Collagen I alpha 1 Antibody [Alexa Fluor® 647]
Available conjugates: Available conjugates: Alexa Fluor 647, mFluor Violet 500 SE, Alexa Fluor 532, mFluor Violet 610 SE, HRP, DyLight 594, DyLight 488, Alexa Fluor 488Sizes Available: 0.1 ml