Fig 2: DEC1 expression increased the promotion effect of H. pylori on cell proliferation. A. The results of CCK-8 continuous detection. B,D. EdU test and quantitative analysis. CE. Colony-forming unit assays and quantitative analysis. F. Annexin V-(7-AAD) staining to assess the apoptosis rate. Each experiment was repeated for three times. EV, empty vector; OV, overexpression vector. t-test, *p < 0.05

Fig 3: Histological investigations of the heart tissues. The myocardial fibers in the sham group were arranged in an orderly fashion. In the Ad-G-Control I/R groups, the myocardial fibers were disorganized and ruptured alongside the presence of edema. Transfection of the DEC1-targeting RNA interference vector partially decreased the myocardial damage. Scale bar=100 µm; magnification, x200. DEC1, deleted in esophageal cancer 1; I/R, ischemia-reperfusion; Ad, adenovirus.

Fig 4: DEC1 expression is positively correlated with H. pylori infection status and the progression of GC. A. GEO RNA sequencing database analysis of the DEC1 expression in H. pylori-infected gastric cells. The data were derived from the GSE00262 and GSE70394. B. qRT-PCR results of cells co-cultured with H. pylori for 4h, 8h and 12h. C. DEC1 protein expression after co-cultured with H. pylori especially at 8h time point. D. DEC1 immunohistochemical stain of GC with or without H. Pylori. Magnification: 40×. E. Associations between DEC1 expression levels and clinicopathological characteristics of H. pylori-positive GC. F. Survival analysis of DEC1 expression in H. pylori-positive GC. All data are presented as mean±standard deviation of at least three independent experiments. Statistic process: t-test, chi-square or Fisher exact tests. *p < 0.05

Fig 5: Delivery of DEC1-targeting RNA interference decreases the increased expression levels of DEC1 induced by I/R. (A) Reverse transcription-quantitative PCR and (B) western blotting were used to analyze the mRNA or protein expression levels, respectively, of DEC1. Data are presented as the mean ± standard deviation (n=5). *P<0.05 vs. sham group; #P<0.05 vs. the I/R group. DEC1, deleted in esophageal cancer 1; I/R, ischemia-reperfusion; Ad, adenovirus.