Fig 1: Plakoglobin knockdown changes the levels of SATB1 target genes. A. Total cellular RNA was isolated from MCF-7 and MCF-7-shPG cells, reverse transcribed and processed for PCR using primers specific to SATB1 target genes c-Abl, MMP3, ErbB2, Snail, BRMS1, Kiss1 and Claudin-1. B. Equal amounts of total cellular proteins from these cells were resolved by SDS-PAGE and processed for immunoblotting with antibodies to c-Abl, MMP3, ErbB2, Snail, BRMS1, Kiss1 and Claudin-1. PG, plakoglobin.