CD163 (ED2) Antibody sc-58965 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemCD163 (ED2) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $333.00Supplier Page View Company Product Page
Catalog Numbersc-58965
Antibody TypePrimary Antibodies
CloneED2
Quantity100 µg/ml
ApplicationsWB, IP, IF, IHC(P), FCM
Reactivitymouse, rat
FormatPurified
Hostmouse
ClonalityMonoclonal
Conjugate/TagUnconjugated
IsotypeIgG1
TargetCD163

Questions and Answers

Can this antibody be used for immunofluorescence staining in macrophage studies?

Yes, the CD163 (ED2) antibody has been successfully used for immunofluorescence staining in studies involving macrophages. For instance, in a study examining the role of mesenchymal stem cells in cardiac inflammation, the antibody was utilized to identify M2 macrophages, indicating its effectiveness in this application ().

Is this antibody effective for detecting CD163 in different experimental models?

The CD163 antibody has demonstrated effectiveness across various experimental models. It has been used in immunohistochemistry to visualize CD163-positive cells in studies related to liver regeneration and macrophage polarization in diabetic nephropathy, showcasing its versatility in different biological contexts (, ).

What species does this antibody react with, and how has it been validated in research?

The CD163 antibody is reactive with mouse and rat species. It has been cited in numerous studies, including those investigating macrophage involvement in cancer metastasis and inflammatory responses, confirming its utility in these species (, ).

How has this antibody been utilized in the context of macrophage polarization?

The CD163 antibody has been employed to assess macrophage polarization, particularly in studies focusing on M2 macrophages. For example, it was used to visualize M2 macrophages in a study investigating the effects of drug treatments on macrophage populations in a murine model of medication-related osteonecrosis ().

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) LIF and Oncostatin M mitigate LPS-induced acute endometritis via distinct immunoregulatory mechanisms in peritoneal macrophages in vivoiScienceJune 19, 2026Marion Ravelojaona, Julie Girouard, Cathy Vaillancourt, Céline Van Themsche, Carlos Reyes-MorenoUSA)",#5359 Mouse monoclonal antibodies targeting CD163,"Santa Cruz Biotechnology (Santa Cruz, CA, USA)",ED2: sc-58965 Anti-mouse IgG Alexa Fluor 594,"Santa Cruz Biotechnology (Santa Cruz, CA, USA)",8890S Anti-rabbit

(2) Engineered haptoglobin β fusion protein targets myoglobin and ameliorates rhabdomyolysis-associated acute kidney injuryEMBO Molecular MedicineMay 25, 2026Ning Li, Yuru Wang, Lu Han, Ou Qiao, Xinyue Wang, Herui Hao, Xin Chen, Pengtao Wang, Sania Saeed, Jing Wang, et al.above treatment. IP with IgG control antibody (Solarbio, #SP031), anti-CD163 antibody (Santa Cruz, #sc-58965), anti-Mb antibody (Abcam, #ab77232), or anti-Hp antibody (HUABIO, #ET1703-24). WCL, whole cell lysate.

(3) Dual-targeted lipid nanoparticles for TET3 siRNA delivery: nanobiotechnology strategy to remodel tumor immune microenvironment in hepatocellular carcinomaJournal of NanobiotechnologyApril 1, 2026Wei Peng, Jin Zhou, Zhiliang Chen, Chengjia Tang, Jia Kuang, Wenke Li, Zhao Yang(1:100; ab153724, Abcam, UK), rabbit anti-IRF4 (1:100; ab315394, Abcam, UK), and mouse anti-CD163 (1:100; sc-58965, Santa Cruz, USA). After PBS washing, sections were incubated with Cy3-conjugated goat anti-rabbit (1:1000;

(4) Nephroprotective effects of visnagin through modulation of macrophage polarization, oxidative stress, inflammation and apoptosis in renal I/R injuryOpen Med (Wars)March 9, 2026Suleyman Sagir, Ugur Seker, Merve Pekince-Ozoner, Meral Yuksel, Gul Sahika Gokdemir, Seval Kaya, Mehmet DemirDallas, TX, USA; Catalog no: sc-28347) and CD163 (Santa Cruz Biotechnology, Dallas, TX, USA; Catalog no: sc-58965) [26]. The samples were deparaffinized in xylene, rehydrated through a graded series of alcohols, and

(5) Apatite-coated implant surfaces exhibit superior biological, immunological, and mechanical properties compared to sandblasted acid-etched surfacesScientific ReportsJanuary 18, 2026Hee‑seung Han, Jaehyuk Hwang, Seung Hye Lee, Yongjoon Kim, Sungtae Kim, Young-Dan Chothe following primary antibodies: CD11c (1:250, Cat# PA5-90208, Invitrogen, USA), CD163 (1:250, Cat# SC-58965, Santa Cruz, USA), and VEGF (1:100, Cat# SC-7269, Santa Cruz, USA). Subsequently, samples were incubated

(6) Acetylbinankadsurin A Decreases Macrophage Glycolysis and Pro-Inflammatory Phenotype Polarization via Inhibiting HIF-1α to Alleviate Hepatic Fibrosis in MiceMolecules (Basel, Switzerland)November 27, 2025Yao Q, Peng W, Lu H, Yang Y, Rao Z, Xie X, Huang D, Wang W, Yan J, Liu J.#sc-28347; Immunoway, San Jose, CA, USA, #YM8023), CD163 (Selleck, Houston, TX, USA, #F1548; Santa Cruz, #sc-58965; Abcam, Cambridge, UK, #ab182422), β-actin (Proteintech, Wuhan, China, #66009-1-IG), β-tubulin (Proteintech,

(7) Bioinstructive scaffolds enhance stem cell engraftment for functional tissue regenerationNature MaterialsSeptember 1, 2025Di Wu, Ioannis Eugenis, Caroline Hu, Soochi Kim, Abhijnya Kanugovi, Shouzheng Yue, Joshua R. Wheeler, Iman Fathali, Sonali Feeley, Joseph B. Shrager, et al.1:50), anti-CD206 (Thermo Fisher Scientific, 18704-1-AP; 1:100), anti-CD163 (Santa Cruz Biotechnology, sc-58965; 1:100), anti-CD31 (BD, 550274; 1:100), anti-CD31 (Abcam, ab28364; 1:100), anti-CD45 (BioLegend, 103112,

(8) Leukocyte-type 12/15-lipoxygenase is essential for timely inflammation-resolution and effective tissue regeneration following skeletal muscle injuryBIORXIVMay 17, 2025Binayok Sharma, Xinyue Lu, Hamood Rehman, Vandré C. Figueiredo, Carol Davis, Holly Van Remmen, Shihuan Kuang, Susan V. Brooks, Krishna Rao Maddipati, James F. MarkworthF1.652s, 1:20), Ly6G (GR1) (Bio-Rad, MCA2387, 1:50), CD68 (Bio-Rad, MCA1957, 1:200), CD163 (Santa Cruz, sc-58965, 1:200), and laminin (Abcam, ab7463, 1:200). Primary antibody staining was visualized with appropriate

(9) The M1/M2 Macrophage Polarization and Hepatoprotective Activity of Quercetin in Cyclophosphamide-Induced Experimental Liver ToxicityVet Med SciJanuary 10, 2025Ugur Seker, Emre Uyar, Gul Sahika Gokdemir, Deniz Evrim Kavak, Sevgi Irtegun-Kandemir1/50; catalog no: sc‐28347) and CD163 (Santa Cruz Biotechnology; dilution ratio: 1/50; catalog no: sc‐58965) were performed with routine immunohistochemistry protocols (Kurt et al. 2023). Briefly, the deparaffinized,

(10) Controlled release of hydrogel-encapsulated mesenchymal stem cells-conditioned medium promotes functional liver regeneration after hepatectomy in metabolic dysfunction-associated steatotic liver diseaseStem Cell Research & TherapyNovember 4, 2024Naoya Kasahara, Takumi Teratani, Junshi Doi, Shinichiro Yokota, Kentaro Shimodaira, Yuki Kaneko, Hideyuki Ohzawa, Yasunaru Sakuma, Hideki Sasanuma, Yasuhiro Fujimoto, et al.Cell Signaling Technology, Danvers, MA), anti-CD31 antibody (ab182981, Abcam, Cambridge, UK), anti-CD163 antibody (sc-58965, Santa Cruz Biotechnology), and anti-E-cadherin antibody (20,874-1-AP, Proteintech) at 4℃ overnight.

(11) Polyphenol-mediated redox-active hydrogel with H2S gaseous-bioelectric coupling for periodontal bone healing in diabetesNature CommunicationsOctober 21, 2024Xinyi Fang, Jun Wang, Chengxinyue Ye, Jiu Lin, Jinhui Ran, Zhanrong Jia, Jinglei Gong, Yiming Zhang, Jie Xiang, Xiong Lu, et al.rabbit anti-iNOS (1:100; Catalog number: ab15323; Abcam), mouse anti-CD163 (1:100; Catalog number: sc-58965; Santa Cruz), rat anti-CD90 (1:100; Catalog number: ab3105; Abcam), rabbit anti-CD31(1:500; Catalog

(12) M1 macrophages as promising agents for cell therapy of endometriosisHeliyonAugust 30, 2024Daria Artemova, Polina Vishnyakova, Andrey Elchaninov, Elena Gantsova, Gennady Sukhikh, Timur Fatkhudinov(sc-18351, Santa Cruz, USA) (1/700), mouse anti-CD86 (ab213044, Abcam, UK) (1/2000), mouse anti-CD1 63 (sc-58965, Santa Cruz, USA) (1/2000), mouse anti-SOCS3 (ab236519, Abcam, UK) (1/2000), rabbit anti-CD206 (ab64693,

(13) Determination of M1/M2 Macrophage Polarization in Ipsilateral and Contralateral Rat Testis Tissue Following Unilateral Torsion/DetorsionReproductive SciencesJuly 1, 2024Merve Kavak Balgetir, Nalan Kaya Tektemur, Ahmet Tektemur, Gaffari Türk, İbrahim Halil Güngör, Aslıhan Cakir Cihangiroglu, Ramazan Fazıl Akkoç, Tuncay Kuloglu, Durrin Ozlem DabakAntibody, sc-20060, Santa Cruz Biotechnology, Inc., California, USA; ED2 Mouse Monoclonal Antibody, sc-58965, Santa Cruz Biotechnology, Inc., California, USA, respectively) in the testis tissue and the determination

(14) Bisphenol A disruption promotes mammary tumor microenvironment via phenotypic cell polarization and inflammatory responseCell biology internationalJune 1, 2023Ruiz TFR, Colleta SJ, Dos Santos DD, Castro NFC, Cabral ÁS, Calmon MF, Rahal P, Gil CD, Girol AP, Vilamaior PSL, et al.

(15) ER stress modulates the immune regulatory ability in gut M2 cells of patients with ulcerative colitisiScienceApril 21, 2023Feng B, Xu L, Song S, Liu H, Li Y, Hu S, Shu Q, Liu J, Liu Z, Yu H, et al.

(16) Acerola fruit by-product alleviates lipid, glucose, and inflammatory changes in the enterohepatic axis of rats fed a high-fat dietFood chemistryMarch 1, 2023Batista KS, Soares NL, Dorand VAM, Alves AF, Dos Santos Lima M, de Alencar Pereira R, Leite de Souza E, Magnani M, Persuhn DC, de Souza Aquino J.

(17) Zoledronate/Anti-VEGF Neutralizing Antibody Combination Administration Increases Osteal Macrophages in a Murine Model of MRONJ Stage 0-like LesionsJournal of clinical medicineFebruary 28, 2023Kaneko H, Kuroshima S, Kozutsumi R, Al-Omari FA, Hayano H, Nakajima K, Sawase T.anti-mouse monoclonal antibody to F4/80 at 1:25 (ab16911, Abcam) and mouse anti-rat CD163 antibody at 1:50 (sc-58965, Santa Cruz Biotechnology), followed by the cocktail of the Alexa Fluor 546 goat anti-rat IgG and goat anti-mouse IgG (H&L) (FITC

(18) Lipid nanoparticles for antisense oligonucleotide gene interference into brain border-associated macrophagesFront Mol BiosciNovember 3, 2022Macarena Calero, Lara H. Moleiro, Aline Sayd, Yeray Dorca, Lluis Miquel-Rio, VerÃ³nica Paz, Javier Robledo-MontaÃ±a, Eduardo Enciso, Fernando AcciÃ³n, Diego HerrÃ¡ez-Aguilar, et al.solution, the sections were incubated with antisera for a mouse monoclonal anti-CD163 (ED-2) antibody (sc58965, 1:200; Santa Cruz Biotechnology®) during 2 h at room temperature. Subsequently, the sections were washed

(19) Oceanimonas sp. BPMS22-derived protein protease inhibitor induces anti-leishmanial immune responses through macrophage M2 to M1 repolarizationInternational immunopharmacologyNovember 1, 2022Jayaraman A, Srinivasan S, Kar A, Harish BS, Charan Raja MR, Uppuluri KB, Kar Mahapatra S.

(20) Anti-Metastatic Function of Extracellular Vesicles Derived from Nanog-Overexpressing MelanomaCurrent oncology (Toronto, Ont.)February 11, 2022Hatakenaka T, Matsuki N, Minagawa S, Khoo CSM, Saito M.washed twice with PBS containing 3% BSA. Then, the cells were reacted with anti-CD163 antibody (1:10, sc-58965, Santa Cruz) at room temperature for 1 h and subsequently with goat anti-mouse IgG (H + L) Alexa Fluor

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CD163 (ED2) Antibody

CD163 (ED2) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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CD163 (ED2) Antibody from Santa Cruz Biotechnology, Inc.

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