Alexa Fluor(R) 488 Goat anti-mouse IgG (minimal x-reactivity) 405319 from BioLegend

Product Specs
ItemAlexa Fluor(R) 488 Goat anti-mouse IgG (minimal x-reactivity)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number405319
Quantity100 µg
ApplicationsFlow Cytometry (FC), Immunohistochemistry (IHC)-F
Concentration0.50 mg/mL
Conjugate/TagAlexa Fluor® 488
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ReactivityMouse
IsotypeIgG
ClonePoly4053
HostGoat
Antibody TypeSecondary
NCBI Full Gene Nameimmunoglobulin heavy chain (V7183 family)
NCBI Gene AliasesB9-scFv, IgG, IgH, IgVH1(VSG), VH7183, VI24H
ClonalityPolyclonal
TargetIgG

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Website: biolegend.com

(1) Transcriptional inhibition after irradiation occurs preferentially at highly expressed genes in a manner dependent on cell cycle progressionbioRxivNovember 23, 2023Zulong Chen, Xin Wang, Xinlei Gao, Nina Arslanovic, Kaifu Chen, Jessica Tylerincubated with secondary antibody diluted in 3% BSA PBS (Alexa Fluor 488 Goat anti-mouse IgG, BioLegend, 405319) in the dark for 1 hour at room temperature and washed 3x with PBST. Click-iT reaction cocktail was

(2) Neoplastic synovial lining cells that coexpress podoplanin and CD90 overproduce CSF‐1, driving tenosynovial giant cell tumorJournal of Orthopaedic ResearchAugust 1, 2022Andrew C. Chandler, Mohamed Yakoub, Tomohiro Fujiwara, Laura T. Donlin, Paul Edward Purdue, John H. Healeyspecies,Dilution,Company/catalog number ,,,,,,,, CD90,Mouse,1:500,Biolegend #328101,Anti-mouse,Alexa 488,Goat,1:1000,Biolegend #405319 PDPN,Rat,1:500,Biolegend #337001,Anti-rat,Alexa 647,Goat,1:1000,Biolegend #405416 CSF-1,Rabbit,1:125,Abcam

(3) Malin restoration as proof of concept for gene therapy for Lafora diseaseBrain CommunicationsJuly 4, 2022Olga Varea, Joan J Guinovart, Jordi DuranThermoFisher); anti-mouse Alexa Fluor 568 (A11031, Invitrogen); or/and anti-mouse IgG Alexa Fluor 488 (405319, Biolegend); and anti-guinea pig Cy3, in combination with DAPI (Sigma). For brightfield labelling, the

(4) Translational development of a tumor junction opening technologyScientific reportsMay 11, 2022Kim J, Li C, Wang H, Kaviraj S, Singh S, Savergave L, Raghuwanshi A, Gil S, Germond A, Baldessari A, et al.donkey anti-goat IgG (Abcam #6881), and Alexa Fluor 488-conjugated goat anti-mouse IgG (Biolegend #405319). Cytokine cytometric bead array Animal sera were taken and assessed for cytokines (Th1/Th2) using

(5) Expression of the mono-ADP-ribosyltransferase ART1 by tumor cells mediates immune resistance in non-small cell lung cancerScience translational medicineMarch 16, 2022Wennerberg E, Mukherjee S, Spada S, Hung C, Agrusa CJ, Chen C, Valeta-Magara A, Rudqvist NP, Van Nest SJ, Kamel MK, et al.buffer and resuspended in AF488-conjugated Goat anti-mouse IgG secondary antibody (BioLegend, Catalog#405319) for 20 minutes at 4°C. Cells were washed in FACS buffer and incubated with a master mix of fluorophore-conjugated

(6) MBL Binding with AhR Controls Th17 Immunity in Silicosis-Associated Lung Inflammation and FibrosisJournal of inflammation researchJanuary 1, 2022Liu Y, Zhao N, Xu Q, Deng F, Wang P, Dong L, Lu X, Xia L, Wang M, Chen Z, et al.anti-human/mouse AhR (67785-1-Ig, Proteintech, Chicago, USA), Alexa Fluor® 488 goat anti-mouse IgG antibody (405319, Biolegend, San Diego, USA). Cells were analyzed using the FACS LSRFortessa™ flow cytometer (BD Biosciences)

(7) Rearrangement of Actin Cytoskeleton by Zika Virus Infection Facilitates Blood-Testis Barrier HyperpermeabilityVirologica SinicaAugust 1, 2021Nie Y, Hui L, Guo M, Yang W, Huang R, Chen J, Wen X, Zhao M, Wu Y.donkey anti-rabbit IgG antibody (406416, Biolegend), Alexa Fluor 488 goat anti-mouse IgG antibody (405319, Biolegend). 4′, 6-diamidino-2-phenylindole (DAPI, P0131, Beyotime) was used to dye the nuclei. The

(8) Characterisation of Neurospheres-Derived Cells from Human Olfactory EpitheliumCellsJuly 4, 2021Elena A. Zelenova, Nikolay V. Kondratyev, Tatyana V. Lezheiko, Grigoriy Y. Tsarapkin, Andrey I. Kryukov, Alexander E. Kishinevsky, Anna S. Tovmasyan, Ekaterina D. Momotyuk, Erdem B. Dashinimaev, Vera E. Golimbet(Thermo Fisher Scientific, Waltham, MA, USA); Alexa Fluor® 488 Goat anti-mouse IgG (minimal x-reactivity) Antibody, cat. 405319 (Biolegend, San Diego, CA, USA). 2.1.3. RNA-seq RNeasy column kits, cat. 74104 (Qiagen, Hilden, Germany);

(9) In Vitro Implantation Model Using Human Endometrial SUSD2+ Mesenchymal Stem Cells and Myometrial Smooth Muscle CellsCell JMay 26, 2021Marzieh Rahimipour, Mina Jafarabadi, Mojdeh Salehniawere incubated with secondary goat anti-mouse polyclonal antibody conjugated with Alexa Fluor® 488 (405319, 1:100 in PBS, Biolegend, UK) for 2 hours at 37˚C and washed three times with PBS. Nuclei were counterstained

(10) A Novel Autoantibody Induced by Bacterial Biofilm Conserved Components Aggravates Lupus NephritisFrontiers in ImmunologyMarch 25, 2021Wenyan Fu et al.1 h and stained with goat anti-mouse IgG antibody labeled with Alexa Fluor® 488 (1:100; Biolegend, 405319) for 1 h. Images were acquired using PE UltraView VoX Turntable Confocal Microscope according to manufacturer

(11) Control of oviductal fluid flow by the G-protein coupled receptor Adgrd1 is essential for murine embryo transitNature communicationsFebruary 23, 2021Bianchi E, Sun Y, Almansa-Ordonez A, Woods M, Goulding D, Martinez-Martin N, Wright GJ.Alexa Fluor 633 goat anti-mouse IgG (Thermo Fisher A-21052)1:500; Alexa Fluor 488 goat anti-mouse IgG (405319 Biolegend) 1:500; Alexa Fluor 488 goat anti-rabbit IgG (111-545-003 Jackson Immunoresearch) 1:500; pentameric

(12) Prx1-expressing cells contributing to fracture repair require primary cilia for complete healing in miceBoneFebruary 1, 2021Emily R. Moore, O. Amandhi Mathews, Yichen Yao, Yingzi YangFluor 568 goat anti-rabbit (Life Technologies, A11011) and Alexa Fluor 488 goat anti-mouse (BioLegend, 405319). Images were acquired using an inverted fluorescent microscope (ECHO Revolve 4). To quantify primary cilium

(13) Suppression of glycogen synthesis as a treatment for Lafora disease: Establishing the window of opportunity.Neurobiology of DiseaseJanuary 1, 2021Olga Varea, Jordi Duran, Mònica Aguilera, Neus Prats, Joan J. GuinovartThermoFisher); anti-Mouse Alexa Fluor 568 (A11031, Invitrogen); or/and anti-mouse IgG Alexa Fluor 488 (405319, Biolegend), in combination with DAPI (Sigma). The samples were mounted in fluorescence mounting media

(14) Human physiomimetic model integrating microphysiological systems of the gut, liver, and brain for studies of neurodegenerative diseasesScience advancesJanuary 1, 2021Trapecar M, Wogram E, Svoboda D, Communal C, Omer A, Lungjangwa T, Sphabmixay P, Velazquez J, Schneider K, Wright CW, et al.concurrently stained with an Alexa Fluor 488 secondary goat anti-mouse antibody (BioLegend, catalog no. 405319), NucBlue (Invitrogen, catalog no. 12303553), and ActinRed (Invitrogen, catalog no. 15119325). The membranes

(15) Small-molecule-induced polymerization triggers degradation of BCL6NatureDecember 1, 2020Słabicki M, Yoon H, Koeppel J, Nitsch L, Roy Burman SS, Di Genua C, Donovan KA, Sperling AS, Hunkeler M, Tsai JM, et al.anti-mouse Alexa Fluor 633 (ThermoFisher Scientific, A-21052), and Alexa anti-mouse 488 (Biolegend, 405319). Whole proteome quantification using tandem mass tag mass spectrometry 10 × 106 SuDHL4Cas9 cells

(16) Lack of Astrocytic Glycogen Alters Synaptic Plasticity but Not Seizure SusceptibilityMolecular NeurobiologyNovember 1, 2020Jordi Duran, M. Kathryn Brewer, Arnau Hervera, Agnès Gruart, Jose Antonio del Rio, José M. Delgado-García, Joan J. GuinovartMerck Millipore) was diluted 1:1000 and incubated overnight at 4°C. Alexa Fluor 488 Goat anti-mouse (405319, Biolegend) was used as the secondary antibody. Samples were stained with DAPI (D9542, Sigma) and mounted

(17) Osteoprotegerin Prevents Intracranial Aneurysm Progression by Promoting Collagen Biosynthesis and Vascular Smooth Muscle Cell ProliferationJournal of the American Heart AssociationSeptember 1, 2020Miyata T, Minami M, Kataoka H, Hayashi K, Ikedo T, Yang T, Yamamoto Y, Yokode M, Miyamoto S.Signaling Technology Abcam 1:300 GR3214415-1 Iba-1 Wako 1:200 LKF6437 Osteoprotegerin Novus Biologicals ab18197 01919741 NB10056505 1:200 NOV-9975-27 BioLegend 406416 1:200 B215846 BioLegend 405319 1:200 B213505 BioLegend 406418 1:200 B206209 BioLegend 405326 1:200 B221919 Target antigen ProCOL1A1 ProCOL3A1 Phosphorylated Smad2/3 Smooth muscle

(18) Natural genetic variation in Stim1 creates stroke in the spontaneously hypertensive ratGenes and immunityMay 1, 2020Dhande IS, Kneedler SC, Zhu Y, Joshi AS, Hicks MJ, Wenderfer SE, Braun MC, Doris PA.washing and a 1 h incubation at room temperature with Alexa 488-conjugated goat anti-mouse IgG (Biolegend-405319). Nuclear counterstaining was performed with 5 mM Draq5 (Cell Signaling-4084S) for 1 h. Images were

(19) C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1Scientific reportsApril 14, 2020Alberts A, Klingberg A, Wessig AK, Combes C, Witte T, Brand K, Pich A, Neumann K.IgG-AF568 (H + L) (Thermo Fisher Scientific, #A11036, 4 µg/ml), or anti-mouse IgG-AF488 (BioLegend, #405319, 4 µg/ml) were used as secondary antibodies. After the final washing step, the particles were taken

(20) Gut-Liver Physiomimetics Reveal Paradoxical Modulation of IBD-Related Inflammation by Short-Chain Fatty AcidsCell systemsMarch 25, 2020Trapecar M, Communal C, Velazquez J, Maass CA, Huang YJ, Schneider K, Wright CW, Butty V, Eng G, Yilmaz O, et al.concurrently stained with an AlexaFluor 488 secondary goat anti-mouse antibody (Biolegend, cat. no. 405319), NucBlue (Invitrogen, cat. no. 12303553) and ActinRed (Invitrogen, cat. no. 15119325). Membranes were

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5/19/2016Staining of Mouse Intestinal Tissue yougang zhaiTo analyze the intestinal tumor cells, Min+ mice were sacrificed and tumor polyps from intestinal tissue were collected and snap frozen. Cryo sections were prepared and used for staining the ... Read Review

Alexa Fluor(R) 488 Goat anti-mouse IgG (minimal x-reactivity) from BioLegend

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Alexa Fluor(R) 488 Goat anti-mouse IgG (minimal x-reactivity) from BioLegend

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