Fig 1: The effects of AG on the protein levels of MAPK signaling molecules in MC3T3-E1 cells. MC3T3-E1 cells were treated with AG at 5, 10, and 20 µM for 24 h. (A) The protein levels of p-Erk1/2, Erk1/2, p-JNK, JNK, p-p38, and p38 were measured by western blot. (B) Quantitative analysis of the protein levels of p-Erk1/2 and Erk1/2 in AG-treated MC3T3-E1 cells. (C) Quantitative analysis of the protein levels of p-p38 and p38 in AG-treated mouse MC3T3-E1 cells. (D) Quantitative analysis of the protein levels of p-JNK and JNK in AG-treated mouse MC3T3-E1 cells. (E) Quantitative analysis of the ratios of the phosphorylated proteins to the total proteins in AG-treated mouse MC3T3-E1 cells. All of the data are shown as the mean ± S.D. of the independent experiments. N = 3. *P < 0.05, **P < 0.01 compared with the corresponding blank group. #P < 0.05, ##P < 0.01 compared with the corresponding control group.
Fig 2: TRIM9 regulates cell proliferation and intestinal barrier function in IEC-6 cells likely via the P38 pathway.IEC-6 cells were treated with the TRIM9 overexpression vector combined with P38 inhibitor SB203580. (A) Cell proliferation at 0, 12, 24 and 48 h was detected using a Cell Counting Kit-8 assay. (B) TEER was measured in each group. (C) The fluorescence intensity of FITC was tested in each group. (D) Cell apoptosis was detected with a flow cytometer. (E) The levels of IL-1ß and TNF-a expression were detected with an ELISA. (F) The protein expression levels of ZO-1, Claudin-4 and p-P38/P38 were detected via western blotting. *P<0.05, **P<0.01, ***P<0.001 vs. Vector; #P<0.05, ##P<0.01, ###P<0.001 vs. oeTRIM9 + Vehicle. TRIM9, tripartite motif protein 9; oe, overexpression; TEER, transepithelial electrical resistance; TNF-a, tumor necrosis factor-a; IL-1ß, interleukin-1ß; ZO-1, zona occludens protein 1; p-, phosphorylated.
Fig 3: Knockdown of TREM-1 reduces the signal activation of p38 and NF-?B under DAMP-induced conditions. (a). Western blot detects the changes in p-p38 and p-p65 and p-JNK expression in each group after different treatments. (b). Statistical results of p-p38 and p-p65 and p-JNK expression. **P < 0.01.
Fig 4: PEA15 promotes the development of colorectal cancer by regulating the MAPK signaling pathway. (A) Gene microarray analysis revealed that these genes were involved in a variety of signaling pathways. (B) The expression of PEA15, PLD1, ERK, MAP2K6 and PRKX proteins decreased and the expression of FLNB protein increased in RKO cells after downregulation of PEA15, while the expression of p38 and JNK proteins had no significant changes. *P<0.05 and **P<0.01 compared to controls.
Fig 5: Effects of different interventions on the p38MAPK protein level in placenta tissues of pregnant rats. a indicates that in comparison with the control group, P < 0.05; b indicates that in comparison with the model group, P < 0.05, and c indicates that in comparison with the heparin group, P < 0.05.
Supplier Page from Abcam for Anti-p38 antibody [E229]