Abcam
Anti-p38 antibody [E229]
ab170099
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The hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this pathway results in basal cell carcinoma and medulloblastoma. A unique feature of this pathway is that it requires a tiny antenna like organelle called the primary cilium for signal transduction. My goal is to understand the molecular mechanism of hedgehog signal transduction at the primary cilium. This involves detecting protein-protein interactions of the pathway components, analysis of localization patterns during signal transduction, and transcriptional output measurements. In the present experiment, knockdown of hedgehog pathway components was performed and a marker was needed to judge loading of samples for Western Blotting. p38 blotting was done to check if this antibody works in 3T3 cells so that it can be used at later stages.
Western Blot
NIH-3T3 cell lysate
1:2000 dilution in blocking buffer; 16 hr at 4 deg
5% Milk powder in 1xTBS-T(0.1%)
Donkey anti-rabbit IgG-HRP conjugated; 1:10,000 dilution in blocking buffer
None
ECL regular
p38 levels do not change upon hedgehog pathway manipulation
Specific signal
One of the best p38 antibodies in the market for Western Blotting