Fig 1: Inverse correlation between RND3 gene expression and the expression level of miR-182-5p or miR-96-5p in liver tissue specimens. (A–D): RT-qPCR and western blotting for evaluating the mRNA/protein expression level of RND3 as well as the expression level of two indicated miRNAs in non-HCC tissue specimens (NC), HCC tissue specimens (HCC) and paired adjacent counterparts (adjacent). (E and F): Pearson correlation analysis evaluating the correlation between the expression level of RND3 mRNA and that of the two indicated miRNAs. ***, p < 0.001; ****, p < 0.0001.
Fig 2: RND3 mRNA is a direct target of miR-182-5p and miR-96-5p in HCC cells. (A–E): RND3 mRNA and protein expression level change in patient-derived HCC cells after treatment with indicated agomir or antagomir. (F): Luciferase reporter assay verifying the targeting potential of the two indicated miRNA agomir to RND3 mRNA 3' UTR. (G): AGO2 RIP assay verifying the binding potential of the two indicated miRNA agomir to RND3 mRNA. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Fig 3: RND3 reduced HCC cell mobility partially by inhibiting ROCK1/2. (A and B): mRNA and protein expression level of indicated genes in HCC cells after RND3 knockdown, ROCK1 knockdown or ROCK2 knockdown. (C–F): Trans-well migration and invasion of HCC cells after different modifications and treatment. (G and H): MYPT1 protein expression level as well as its Thr853 phosphorylation (pT853) level in HCC cells in (C and D). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Fig 4: MiR-182-5p or miR-96-5p increased HCC cell proliferation and cisplatin resistance by antagonizing RND3. (A–C): Cell viability, proliferation and apoptosis of HCC cells after indicated modifications and treatment in vitro. (D): Protein expression level of PCNA, caspase-3 and active caspase-3 in HCC treated in (A). (E–H): Cell apoptosis and caspase-3, -8 or -9 activities in HCC cells after indicated modifications and treatment in addition to cisplatin treatment in vitro. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Fig 5: MiR-182-5p or miR-96-5p increased HCC cell mobility in vitro by antagonizing RND3. (A and B): RND3 mRNA and protein expression level in HCC cells after indicated treatment. (C–F): Trans-well migration and invasion of un-modified (NC) or RND3 overexpressing (RND3 OE) HCC cells with or without indicated miRNA agomir treatment. (G and H): MYPT1 protein expression level as well as its Thr853 Phosphorylation (pT853) level in HCC cells in (C and D). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Supplier Page from Abcam for Anti-RND3 antibody