Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) ab175773 from Abcam

Product Specs
ItemGoat Anti-Rabbit IgG H&L (Alexa Fluor® 680)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab175773
Quantity500 µg
ApplicationsWB
Conjugate/TagAlexa Fluor® 680
FormatThe antibody was isolated by affinity chromatography using antigen coupled to agarose beads
Concentration2 mg/ml
TargetRabbit IgG
HostGoat
ClonalityPolyclonal
Antibody TypePrimary Antibody
IsotypeIgG

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Geometry-driven immunomodulation in 3D-printed bioceramics: Negative curvature promotes macrophage M2 polarization via Ras-MAPK/HIF-1α signaling for vascularized osteogenesisBioactive MaterialsJune 1, 2026Qiji Lu, Yudi Kuang, Jingjing Diao, Jiaqian Zheng, Naru Zhao, Chang Du, Yingjun WangChina), BSA (B2064, ≥98 %, Sigma-Aldrich, USA.),CD31 antibody (ab28364, Abcam, USA.), secondary anti-IGg (ab175773, Alexa Fluor® 680, Abcam, USA.), Phalloidin-iFluor 488(ab176753, Abcam, USA.), CCR7(AF5293, Bioss, China),

(2) FAM162A Is a Key Regulator of Mitochondrial Structure, Dynamics, and Bioenergetics, Driving Cellular Protection and LongevityAging CellMay 10, 2026Andrea Matamoros, Juan Pablo Soffia, Marcelo Muñoz, Michael Maturana, Ma Andreina Rangel-Ramirez, Alvaro Gonzalez-Ibañez, Gabriela Gómez-Lillo, Cesar Astorga, Lina M. Ruiz, Ramón A. Jorquera, et al.HrP,7076,Cell Signaling Technology,goat,1:2500, Mouse IgG AF680,ab175774,Abcam,goat,1:5000, Rabbit IgG AF680,ab175773,Abcam,goat,1:5000, Rabbit IgG (H + L) HrP,"31,460",Invitrogen,goat,1:10000, Antibodies used in this

(3) Regulatory effects of soy isoflavones on ovarian development in female Chinese mitten crabs (Eriocheir sinensis)Animal NutritionMarch 14, 2026Long He, Jinping Li, Dexiang Cao, Zhijun Liu, Chuanjie Qin, Xiaodan Wang, Jianguang Qin, Erchao Li, Liqiao Chenfor 30 min, they were incubated with the corresponding secondary antibodies (1:10,000; ab175775 and ab175773, Abcam Plc, Cambridge, UK) for 1 h. Then, the images were scanned and photographed using an Odyssey CLx

(4) Structures of nucleotide-bound human telomerase at several steps of its telomeric DNA repeat addition cycleNature CommunicationsJanuary 21, 2026Sebastian Balch, Elsa Franco-Echevarría, George E. Ghanim, Rachael C. Kretsch, Rhiju Das, Thi Hoang Duong Nguyenprobed with secondary antibodies (1:5000 dilution): either goat anti-Rabbit Alexa Fluor 680 (Abcam, Cat# ab175773, lot GR222353-8) or goat anti-Mouse Alexa Fluor 680 (Abcam, Cat# ab175775, lot GR3273649-2). Following

(5) Verbascoside targets endothelial HIF-1α/ Lysyl oxidase signaling to attenuate glomerular injury in diabetic nephropathyRedox RepDecember 8, 2025Tianyu Kang, Bin Hou, Min Shi, Huan Liu, Yanan Li, Kaixin Li, Shuxin Li, Zetong Wu, Zhaopeng Xu, Mengnan Limembranes were incubated for 1 hour at room temperature with secondary antibodies: anti-rabbit IgG (Abcam, ab175773, 1:10,000) or anti-mouse IgG (Abcam, ab175737, 1:10,000). Protein bands were visualized using an Odyssey CLX imaging system

(6) Cadherin 11 regulates presynaptic vesicle trafficking and neuronal activity in autism-related brain circuitMolecular psychiatryAugust 12, 2025Cao X, Jia J, Li Z, Guo S, Duan J, Ma J, Yuan XB, Pan YH.antibodies: goat-anti-mouse antibody (1:1000, #ab216772, Abcam) or goat anti-rabbit antibody (1:1000, #ab175773, Abcam) for 2 h at RT and washed with TBST. Protein bands on the blots were visualized using BeyoECL

(7) Cryo-EM structure of human telomerase dimer reveals H/ACA RNP-mediated dimerizationScienceJuly 10, 2025Sebastian Balch, Zala Sekne, Elsa Franco-Echevarría, Patryk Ludzia, Rachael C. Kretsch, Wenqing Sun, Haopeng Yu, George E. Ghanim, Sigurdur Thorkelsson, Yiliang Ding, et al.lot 1041820-24) was used as primary antibody. Goat anti-rabbit Alexa-Fluor 680 (1:5,000, Abcam, Cat# ab175773, lot GR222353-8) was used as a secondary antibody. Total TERT levels for each glycerol gradient fraction

(8) Host cellular protein RAB33B facilitates influenza viral replication and modulates M2 trafficking by enhancing autophagyVet ResJuly 1, 2025Shaotang Ye, Zhen Wang, Gang Lu, Aolei Chen, Liang Xu, Yongbo Liu, Jianwei Mao, Jingyu Wang, Gaoming Lou, Qingmei Xie, et al.1:1000); ATG16L1 (AB187671, 1:1000), LC3 (AB192890, 1:2000), P62 (AB109012, 1:1000), Alexa Fluor® 680 (AB175773, 1:5000), Alexa Fluor® 790 (AB175781, 1:5000), Alexa Fluor® 405 (AB175651, 1:1000), Alexa Fluor® 488

(9) Multi-omics integration reveals Vha68-3 as a testicular aging-specific factor that coordinates spermatid elongation through mitochondrial metabolic homeostasisCell Mol Biol LettMay 9, 2025Jun Yu, Qiuru Huang, Yangbo Fu, Lei He, Cong Shen, Xia Chen, Zhiran Li, Jiaxin Li, Chenyu Wang, Xinda Wang, et al.Beyotime). After washing, membranes were incubated with fluorescent secondary antibodies (Alexa Fluor® 680, ab175773, Abcam; IRDye® 800, ab216772, Abcam) and visualized using an Amersham Typhoon 5 biomolecular imager

(10) GPC3-mediated lysosome-targeting chimeras (GLTACs) for targeted degradation of membrane proteinsActa Pharmaceutica Sinica BMarch 8, 2025Yuxin Fang, Yaojin Zhu, Wei Wang, Zhewei Xia, Shipeng He, Guoqiang Dong, Chunquan Shengto label the PD-L1 monoclonal antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (Abcam, Cat No. ab175773) (1:200, 1 mL) was added to label the Glypican 3 polyclonal antibody respectively. After incubating

(11) Bone‐Targeted Fluoropeptide Nanoparticle Inhibits NF‐κB Signaling to Treat Osteosarcoma and Tumor‐Induced Bone DestructionAdvanced ScienceNovember 6, 2024Lin Li, Guangyu Rong, Xin Gao, Yiyun Cheng, Zhengwang Sun, Xiaopan Cai, Jianru XiaoAcquired from Abcam (UK), the rabbit monoclonal antibody to GAPDH (ab181602), goat anti‐rabbit IgG H&L (ab175773), and goat anti‐mouse IgG H&L (ab175775) were utilized. Bone slices were purchased form IDS PLC (DT‐1BON1000‐96,

(12) Map-1a regulates Sertoli cell BTB dynamics through the cytoskeletal organization of microtubule and F-actinReproductive Biology and EndocrinologyApril 3, 2024Lingling Wang, Ming Yan, Tiao Bu, Xiaolong Wu, Linxi Li, Bruno Silvestrini, Fei Sun, C. Yan Cheng, Hao ChenGoat Anti-Rabbit IgG H&L(IRDye® 800),Rabbit,Abcam,ab216773,1:3000,— Goat Anti-Rabbit IgG H&L(Alexa Fluor® 680),Rabbit,Abcam,ab175773,1:3000,— Goat Anti-Mouse IgG H&L(IRDye® 800),Mouse,Abcam,ab216772,1:3000,— Rhodamine Phalloidin(Molecular

(13) FOXM1 promote the growth and metastasis of uveal melanoma cells by regulating CDK2 expressionInternational OphthalmologyFebruary 11, 2024Xue Bai, Shan Li, Yan LuoProteintech) overnight at 4 °C. Subsequently, Goat Anti-Rabbit IgG H&L secondary antibody (Alexa Fluor® 680) (ab175773, 1:20,000, Abcam, Cambridge, UK) was utilized to interact with the membrane for 2 h in indoor environment.

(14) 2.7 Å cryo-EM structure of human telomerase H/ACA ribonucleoproteinNature CommunicationsJanuary 25, 2024George E. Ghanim, Zala Sekne, Sebastian Balch, Anne-Marie M. van Roon, Thi Hoang Duong Nguyenwith PBST, incubated with a secondary antibody (1:10,000 goat anti-rabbit Alexa-Fluor 680, Abcam Cat# ab175773, lot GR222353-8, or 1:10,000 goat anti-mouse Alexa-Fluor 680, Abcam Cat# ab175775, lot GR3273649-2)

(15) A Comparative Study of Different Protocols for Isolation of Murine Neutrophils from Bone Marrow and SpleenInt J Mol SciDecember 8, 2023Khetam Sounbuli, Ludmila A. Alekseeva, Oleg V. Markov, Nadezhda L. MironovaGoat Anti-Rabbit IgG Alexa Fluor® 680 at 1:500 dilution for 1 h at 37 °C (Abcam, Cambridge, UK, Cat#ab175773). After, the coverslips were washed with PBS twice and treated with a lipophilic dye DIOC6 solution

(16) Nifuroxazide Activates the Parthanatos to Overcome TMPRSS2:ERG Fusion-Positive Prostate CancerMolecular cancer therapeuticsMarch 2, 2023Li C, Zhang J, Wu Q, Kumar A, Pan G, Kelvin DJ.(Beyotime, AF1201). DAPI was used to stain the nucleus. Goat anti-rabbit IgG H&L Alexa Fluor 680 (Abcam, ab175773) and goat anti-mouse IgG H&L Alexa Fluor 488 (Abcam, ab150113) were used for secondary antibodies, and

(17) Colocalization of senescent biomarkers in deep, superficial, and ovarian endometriotic lesions: a pilot studyScientific ReportsOctober 14, 2022Laura Palmieri, Helena Malvezzi, Bruna Cestari, Sergio PodgaecAnti-197 Rabbit IgG H&L [ab150077], Abcam, Cambridge, UK) and Alexa Fluor® 680 (goat anti-rabbit IgG H&L [ab175773], Abcam, Cambridge, UK) for primary antibody 1 and conventional fluorescein (FITC—goat anti-mouse polyclonal

(18) Urinary exosomal long non-coding RNAs as noninvasive biomarkers for diagnosis of bladder cancer by RNA sequencingFrontiers in OncologySeptember 1, 2022Bingxian Bian et al.(EXOAB-KIT-1, SBI, USA) at 4°C overnight and subsequently with goat anti-rabbit IgG H&L secondary antibody (ab175773, Abcam, UK) at room temperature for one hour. The fluorescence detection was performed on the the Li-COR

(19) A Similarity-Based Method for Predicting Enzymatic Functions in Yeast Uncovers a New AMP HydrolaseJournal of Molecular BiologyApril 15, 2022Nir Cohen, Amit Kahana, Maya Schuldiner(ab125096, Abcam) and a polyclonal rabbit α-GFP (ab290, Abcam). Secondary antibodies were alexa680 α-rabbit (ab175773, Abcam) and alexa790 α-mouse (ab186695, Abcam) that enable scanning using an Odyssey imaging system

(20) microRNA-223-3p attenuates angiotensin II-dependent ROS effect on cell viability by targeting NLRP3 in H9c2 cellsArchives of Medical ScienceMarch 28, 2021Weiran Dai, Shuang Zhou, Guoqiang Zhong, Zhiyuan Jiangprimary 162 antibodies were washed. Then, membranes were incubated in secondary antibodies (1:10000, 163 ab175773/ab150115; Abcam, Cambridge, UK) for 1 h at room temperature. Odyssey Imaging 164 System (LI-COR Biosciences,

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) from Abcam

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) from Abcam

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