Immunoprecipitation: RFWD3 Antibody [NB100-68206] - Detection of Human RFWD3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RFWD3 antibody NB100-68206 used for IP at 3 mcg/mg lysate. RFWD3 was also immunoprecipitated by rabbit anti-RFWD3 antibody NB100-68207, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.